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Effects of Salts on Internal DNA Pressure and Mechanical Properties of Phage Capsids.

Evilevitch, Alex LU ; Roos, Wouter H; Ivanovska, Irena L; Jeembaeva, Meerim LU ; Jönsson, Bengt LU and Wuite, Gijs J L (2011) In Journal of Molecular Biology 405. p.18-23
Abstract
Based on atomic force microscopy nanoindentation measurements of phage λ, we previously proposed a minimal model describing the effect of water hydrating DNA that strengthens viral capsids against external deformation at wild-type DNA packing density. Here, we report proof of this model by testing the prediction that DNA hydration forces can be dramatically decreased by addition of multivalent ions (Mg(2+) and Sp(4+)). These results are explained using a DNA hydration model without adjustable parameters. The model also predicts the stiffness of other DNA-filled capsids, which we confirm using bacteriophage ϕ29 and herpes simplex virus type 1 particles.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Molecular Biology
volume
405
pages
18 - 23
publisher
Elsevier
external identifiers
  • wos:000286700800003
  • pmid:21035458
  • scopus:78650420324
ISSN
1089-8638
DOI
10.1016/j.jmb.2010.10.039
language
English
LU publication?
yes
id
4e6017dc-d9c8-4320-85fc-b435806e0268 (old id 1732547)
date added to LUP
2010-12-16 14:27:12
date last changed
2017-06-11 04:13:26
@article{4e6017dc-d9c8-4320-85fc-b435806e0268,
  abstract     = {Based on atomic force microscopy nanoindentation measurements of phage λ, we previously proposed a minimal model describing the effect of water hydrating DNA that strengthens viral capsids against external deformation at wild-type DNA packing density. Here, we report proof of this model by testing the prediction that DNA hydration forces can be dramatically decreased by addition of multivalent ions (Mg(2+) and Sp(4+)). These results are explained using a DNA hydration model without adjustable parameters. The model also predicts the stiffness of other DNA-filled capsids, which we confirm using bacteriophage ϕ29 and herpes simplex virus type 1 particles.},
  author       = {Evilevitch, Alex and Roos, Wouter H and Ivanovska, Irena L and Jeembaeva, Meerim and Jönsson, Bengt and Wuite, Gijs J L},
  issn         = {1089-8638},
  language     = {eng},
  pages        = {18--23},
  publisher    = {Elsevier},
  series       = {Journal of Molecular Biology},
  title        = {Effects of Salts on Internal DNA Pressure and Mechanical Properties of Phage Capsids.},
  url          = {http://dx.doi.org/10.1016/j.jmb.2010.10.039},
  volume       = {405},
  year         = {2011},
}