p205, A potential tumor suppressor, inhibits cell proliferation via multiple pathways of cell cycle regulation
Asefa, Benyam ; Dermott, Jonathan M. ; Kaldis, Philipp LU
; Stefanisko, Karen
; Garfinkel, David J.
and Keller, Jonathan R.
(2006)
In FEBS Letters
580(5).
p.1205-1214
- Abstract
p205 is a member of the interferon-inducible p200 family of proteins that regulate cell proliferation. Over-expression of p205 inhibits cell growth, although its mechanism of action is currently unknown. Therefore, we evaluated the effect of p205 on the p53 and Rb-dependent pathways of cell cycle regulation. p205 expression results in elevated levels of p21, and activates the p21 promoter in vitro in a p53-dependent manner. In addition, p205 induces increased expression of Rb, and binds directly to Rb and p53. Interestingly, p205 also induces growth inhibition independent of p53 and Rb by delaying G2/M progression in proliferating cells, and is a substrate for Cdk2 kinase activity. Finally, we have identified other binding partners of... (More)
p205 is a member of the interferon-inducible p200 family of proteins that regulate cell proliferation. Over-expression of p205 inhibits cell growth, although its mechanism of action is currently unknown. Therefore, we evaluated the effect of p205 on the p53 and Rb-dependent pathways of cell cycle regulation. p205 expression results in elevated levels of p21, and activates the p21 promoter in vitro in a p53-dependent manner. In addition, p205 induces increased expression of Rb, and binds directly to Rb and p53. Interestingly, p205 also induces growth inhibition independent of p53 and Rb by delaying G2/M progression in proliferating cells, and is a substrate for Cdk2 kinase activity. Finally, we have identified other binding partners of p205 by a yeast two-hybrid screen, including the paired homeodomain protein HoxB2. Taken together, our results indicate that p205 induces growth arrest by interaction with multiple transcription factors that regulate the cell cycle, including but not entirely dependent on the Rb- and p53-mediated pathways of growth inhibition.
(Less)
- author
- Asefa, Benyam
; Dermott, Jonathan M.
; Kaldis, Philipp
LU
; Stefanisko, Karen
; Garfinkel, David J.
and Keller, Jonathan R.
- publishing date
- 2006-02-20
- type
- Contribution to journal
- publication status
- published
- keywords
- Cell cycle regulation, Interferon-inducible, p205, p21, p53, Rb
- in
- FEBS Letters
- volume
- 580
- issue
- 5
- pages
- 1205 - 1214
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:16458891
- scopus:32344439914
- ISSN
- 0014-5793
- DOI
- 10.1016/j.febslet.2006.01.032
- language
- English
- LU publication?
- no
- id
- 17334e7d-4a53-461d-922f-346f1a0d7cc7
- date added to LUP
- 2019-09-18 14:24:54
- date last changed
- 2024-01-01 20:41:03
@article{17334e7d-4a53-461d-922f-346f1a0d7cc7,
abstract = {{<p>p205 is a member of the interferon-inducible p200 family of proteins that regulate cell proliferation. Over-expression of p205 inhibits cell growth, although its mechanism of action is currently unknown. Therefore, we evaluated the effect of p205 on the p53 and Rb-dependent pathways of cell cycle regulation. p205 expression results in elevated levels of p21, and activates the p21 promoter in vitro in a p53-dependent manner. In addition, p205 induces increased expression of Rb, and binds directly to Rb and p53. Interestingly, p205 also induces growth inhibition independent of p53 and Rb by delaying G2/M progression in proliferating cells, and is a substrate for Cdk2 kinase activity. Finally, we have identified other binding partners of p205 by a yeast two-hybrid screen, including the paired homeodomain protein HoxB2. Taken together, our results indicate that p205 induces growth arrest by interaction with multiple transcription factors that regulate the cell cycle, including but not entirely dependent on the Rb- and p53-mediated pathways of growth inhibition.</p>}},
author = {{Asefa, Benyam and Dermott, Jonathan M. and Kaldis, Philipp and Stefanisko, Karen and Garfinkel, David J. and Keller, Jonathan R.}},
issn = {{0014-5793}},
keywords = {{Cell cycle regulation; Interferon-inducible; p205; p21; p53; Rb}},
language = {{eng}},
month = {{02}},
number = {{5}},
pages = {{1205--1214}},
publisher = {{Wiley-Blackwell}},
series = {{FEBS Letters}},
title = {{p205, A potential tumor suppressor, inhibits cell proliferation via multiple pathways of cell cycle regulation}},
url = {{http://dx.doi.org/10.1016/j.febslet.2006.01.032}},
doi = {{10.1016/j.febslet.2006.01.032}},
volume = {{580}},
year = {{2006}},
}