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Effects of insulin secretagogues on phospholipid metabolism in pancreatic β-cells

Fex, G. and Lernmark, A. LU orcid (1975) In Biochimica et Biophysica Acta - Lipids and Lipid Metabolism 388(1). p.1-4
Abstract

The effect of insulin secretagogues on the incorporation of [32P]ortho-phosphate into phospholipids was studied in microdissected islets from obese-hyperglycemic mice. Increased 32P-labelling was observed after incubation for 60 min with 10 mM l-leucine, 10 mM l-arginine or 20 mM d-glucose. Most of the label occurred in the phosphatidyl inositol fraction. The effect of l-leucine was additive to that induced by d-glucose while the effect of l-arginine was not. Glibenclamide (0.05 mM) was ineffective whether or not d-glucose was present. The results suggest that there is no direct correlation between the releasing actions of insulin secretagogues and changes in the metabolism of certain phospholipids and that... (More)

The effect of insulin secretagogues on the incorporation of [32P]ortho-phosphate into phospholipids was studied in microdissected islets from obese-hyperglycemic mice. Increased 32P-labelling was observed after incubation for 60 min with 10 mM l-leucine, 10 mM l-arginine or 20 mM d-glucose. Most of the label occurred in the phosphatidyl inositol fraction. The effect of l-leucine was additive to that induced by d-glucose while the effect of l-arginine was not. Glibenclamide (0.05 mM) was ineffective whether or not d-glucose was present. The results suggest that there is no direct correlation between the releasing actions of insulin secretagogues and changes in the metabolism of certain phospholipids and that phospholipid metabolism may be stimulated through more than one mechanism.

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author
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publishing date
type
Contribution to journal
publication status
published
subject
in
Biochimica et Biophysica Acta - Lipids and Lipid Metabolism
volume
388
issue
1
pages
4 pages
publisher
Elsevier
external identifiers
  • scopus:0016767311
  • pmid:804934
ISSN
0005-2760
DOI
10.1016/0005-2760(75)90055-7
language
English
LU publication?
no
id
17366129-fe23-43ea-93a4-d5f54000b2ea
date added to LUP
2019-12-12 23:35:33
date last changed
2024-03-13 08:22:11
@article{17366129-fe23-43ea-93a4-d5f54000b2ea,
  abstract     = {{<p>The effect of insulin secretagogues on the incorporation of [<sup>32</sup>P]ortho-phosphate into phospholipids was studied in microdissected islets from obese-hyperglycemic mice. Increased <sup>32</sup>P-labelling was observed after incubation for 60 min with 10 mM l-leucine, 10 mM l-arginine or 20 mM d-glucose. Most of the label occurred in the phosphatidyl inositol fraction. The effect of l-leucine was additive to that induced by d-glucose while the effect of l-arginine was not. Glibenclamide (0.05 mM) was ineffective whether or not d-glucose was present. The results suggest that there is no direct correlation between the releasing actions of insulin secretagogues and changes in the metabolism of certain phospholipids and that phospholipid metabolism may be stimulated through more than one mechanism.</p>}},
  author       = {{Fex, G. and Lernmark, A.}},
  issn         = {{0005-2760}},
  language     = {{eng}},
  month        = {{04}},
  number       = {{1}},
  pages        = {{1--4}},
  publisher    = {{Elsevier}},
  series       = {{Biochimica et Biophysica Acta - Lipids and Lipid Metabolism}},
  title        = {{Effects of insulin secretagogues on phospholipid metabolism in pancreatic β-cells}},
  url          = {{http://dx.doi.org/10.1016/0005-2760(75)90055-7}},
  doi          = {{10.1016/0005-2760(75)90055-7}},
  volume       = {{388}},
  year         = {{1975}},
}