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Size and Morphology of Assemblies Formed by DNA and Lysozyme in Dilute Aqueous Mixtures

Lundberg, Dan LU ; Carnerup, Anna LU ; Janiak, John LU ; Schillén, Karin LU ; Miguel, Maria da Graça and Lindman, Björn LU (2011) In Physical Chemistry Chemical Physics 13. p.3082-3091
Abstract
Assemblies formed by a well-defined quality of DNA (4331 bp T7 DNA) and the small net-cationic protein lysozyme in dilute aqueous solutions have been characterized using cryo-transmission electron microscopy (cryo-TEM) and dynamic light scattering (DLS) as the main techniques. In a wide range of different DNA to lysozyme ratios in solutions of low ionic strength, dispersions of aggregates with the same general morphology and a practically constant hydrodynamic size are formed. The basic structure formed in the dispersions is that of rather flexible worm-like assemblies with a diameter of 10–20 nm, which are suggested to be made up by bundles of on the order of 10 DNA chains with an intervening matrix of lysozyme. With increased ionic... (More)
Assemblies formed by a well-defined quality of DNA (4331 bp T7 DNA) and the small net-cationic protein lysozyme in dilute aqueous solutions have been characterized using cryo-transmission electron microscopy (cryo-TEM) and dynamic light scattering (DLS) as the main techniques. In a wide range of different DNA to lysozyme ratios in solutions of low ionic strength, dispersions of aggregates with the same general morphology and a practically constant hydrodynamic size are formed. The basic structure formed in the dispersions is that of rather flexible worm-like assemblies with a diameter of 10–20 nm, which are suggested to be made up by bundles of on the order of 10 DNA chains with an intervening matrix of lysozyme. With increased ionic strength, the worm-like appearance of the assemblies is lost and they adopt a less well-defined shape. The results suggest that the formation of the DNA–lysozyme aggregates is strongly influenced by cooperative assembly of the components and that, in addition to the electrostatic attraction between DNA and lysozyme, attractive interactions between the protein units are important in governing the behavior of the system. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Physical Chemistry Chemical Physics
volume
13
pages
3082 - 3091
publisher
Royal Society of Chemistry
external identifiers
  • wos:000287041700011
  • scopus:79951499638
ISSN
1463-9084
DOI
10.1039/C0CP01220C
language
English
LU publication?
yes
id
09ff7844-baeb-41ed-ab27-330ff4e48741 (old id 1737197)
date added to LUP
2010-12-15 12:13:24
date last changed
2017-01-01 05:54:14
@article{09ff7844-baeb-41ed-ab27-330ff4e48741,
  abstract     = {Assemblies formed by a well-defined quality of DNA (4331 bp T7 DNA) and the small net-cationic protein lysozyme in dilute aqueous solutions have been characterized using cryo-transmission electron microscopy (cryo-TEM) and dynamic light scattering (DLS) as the main techniques. In a wide range of different DNA to lysozyme ratios in solutions of low ionic strength, dispersions of aggregates with the same general morphology and a practically constant hydrodynamic size are formed. The basic structure formed in the dispersions is that of rather flexible worm-like assemblies with a diameter of 10–20 nm, which are suggested to be made up by bundles of on the order of 10 DNA chains with an intervening matrix of lysozyme. With increased ionic strength, the worm-like appearance of the assemblies is lost and they adopt a less well-defined shape. The results suggest that the formation of the DNA–lysozyme aggregates is strongly influenced by cooperative assembly of the components and that, in addition to the electrostatic attraction between DNA and lysozyme, attractive interactions between the protein units are important in governing the behavior of the system.},
  author       = {Lundberg, Dan and Carnerup, Anna and Janiak, John and Schillén, Karin and Miguel, Maria da Graça and Lindman, Björn},
  issn         = {1463-9084},
  language     = {eng},
  pages        = {3082--3091},
  publisher    = {Royal Society of Chemistry},
  series       = {Physical Chemistry Chemical Physics},
  title        = {Size and Morphology of Assemblies Formed by DNA and Lysozyme in Dilute Aqueous Mixtures},
  url          = {http://dx.doi.org/10.1039/C0CP01220C},
  volume       = {13},
  year         = {2011},
}