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Local delivery of molecules from a nanopipette for quantitative receptor mapping on live cells

Babakinejad, Babak ; Jönsson, Peter LU ; López Córdoba, Ainara ; Actis, Paolo ; Novak, Pavel ; Takahashi, Yasufumi ; Shevchuk, Andrew ; Anand, Uma ; Anand, Praveen and Drews, Anna , et al. (2013) In Analytical Chemistry 85(19). p.42-9333
Abstract

Using nanopipettes to locally deliver molecules to the surface of living cells could potentially open up studies of biological processes down to the level of single molecules. However, in order to achieve precise and quantitative local delivery it is essential to be able to determine the amount and distribution of the molecules being delivered. In this work, we investigate how the size of the nanopipette, the magnitude of the applied pressure or voltage, which drives the delivery, and the distance to the underlying surface influences the number and spatial distribution of the delivered molecules. Analytical expressions describing the delivery are derived and compared with the results from finite element simulations and experiments on... (More)

Using nanopipettes to locally deliver molecules to the surface of living cells could potentially open up studies of biological processes down to the level of single molecules. However, in order to achieve precise and quantitative local delivery it is essential to be able to determine the amount and distribution of the molecules being delivered. In this work, we investigate how the size of the nanopipette, the magnitude of the applied pressure or voltage, which drives the delivery, and the distance to the underlying surface influences the number and spatial distribution of the delivered molecules. Analytical expressions describing the delivery are derived and compared with the results from finite element simulations and experiments on delivery from a 100 nm nanopipette in bulk solution and to the surface of sensory neurons. We then developed a setup for rapid and quantitative delivery to multiple subcellular areas, delivering the molecule capsaicin to stimulate opening of Transient Receptor Potential Vanilloid subfamily member 1 (TRPV1) channels, membrane receptors involved in pain sensation. Overall, precise and quantitative delivery of molecules from nanopipettes has been demonstrated, opening up many applications in biology such as locally stimulating and mapping receptors on the surface of live cells.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animals, Capsaicin, Cells, Cultured, Ganglia, Spinal, Nanotechnology, Particle Size, Rats, Rats, Sprague-Dawley, Surface Properties, TRPV Cation Channels, Journal Article, Research Support, Non-U.S. Gov't
in
Analytical Chemistry
volume
85
issue
19
pages
10 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:24004146
  • scopus:84884993254
ISSN
1520-6882
DOI
10.1021/ac4021769
language
English
LU publication?
yes
id
173d876b-d4f2-4dfc-a6db-b85fb1a26bba
date added to LUP
2018-01-26 10:27:19
date last changed
2024-05-27 06:18:14
@article{173d876b-d4f2-4dfc-a6db-b85fb1a26bba,
  abstract     = {{<p>Using nanopipettes to locally deliver molecules to the surface of living cells could potentially open up studies of biological processes down to the level of single molecules. However, in order to achieve precise and quantitative local delivery it is essential to be able to determine the amount and distribution of the molecules being delivered. In this work, we investigate how the size of the nanopipette, the magnitude of the applied pressure or voltage, which drives the delivery, and the distance to the underlying surface influences the number and spatial distribution of the delivered molecules. Analytical expressions describing the delivery are derived and compared with the results from finite element simulations and experiments on delivery from a 100 nm nanopipette in bulk solution and to the surface of sensory neurons. We then developed a setup for rapid and quantitative delivery to multiple subcellular areas, delivering the molecule capsaicin to stimulate opening of Transient Receptor Potential Vanilloid subfamily member 1 (TRPV1) channels, membrane receptors involved in pain sensation. Overall, precise and quantitative delivery of molecules from nanopipettes has been demonstrated, opening up many applications in biology such as locally stimulating and mapping receptors on the surface of live cells.</p>}},
  author       = {{Babakinejad, Babak and Jönsson, Peter and López Córdoba, Ainara and Actis, Paolo and Novak, Pavel and Takahashi, Yasufumi and Shevchuk, Andrew and Anand, Uma and Anand, Praveen and Drews, Anna and Ferrer-Montiel, Antonio and Klenerman, David and Korchev, Yuri E}},
  issn         = {{1520-6882}},
  keywords     = {{Animals; Capsaicin; Cells, Cultured; Ganglia, Spinal; Nanotechnology; Particle Size; Rats; Rats, Sprague-Dawley; Surface Properties; TRPV Cation Channels; Journal Article; Research Support, Non-U.S. Gov't}},
  language     = {{eng}},
  month        = {{10}},
  number       = {{19}},
  pages        = {{42--9333}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Analytical Chemistry}},
  title        = {{Local delivery of molecules from a nanopipette for quantitative receptor mapping on live cells}},
  url          = {{http://dx.doi.org/10.1021/ac4021769}},
  doi          = {{10.1021/ac4021769}},
  volume       = {{85}},
  year         = {{2013}},
}