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Expression of lactate dehydrogenases A and B during chicken spermatogenesis: characterization of testis specific transcripts.

Martinez Arias, Wilma LU ; Mezquita, C and Mezquita, J (2000) In Journal of Cellular Biochemistry 79(1). p.15-27
Abstract
The substrates required for glycolysis change markedly at successive stages of spermatogenesis suggesting a considerable plasticity in the expression of glycolytic enzymes. Lactate dehydrogenase (LDH) isoenzymes, LDH-A and LDH-B, are expressed in premeiotic, meiotic cells, and early spermatids, both in avian and mammalian spermatogenesis. Highly polyadenylated forms, particularly of LDH-A, were detected in chicken testis. While mammals and columbid birds express the testis specific LDH-C gene in meiotic and postmeiotic cells, several LDH-B testis specific transcripts were detected in the corresponding cells during chicken spermatogenesis. These testis specific transcripts and the mRNA of mammalian LDH-C show several properties in common,... (More)
The substrates required for glycolysis change markedly at successive stages of spermatogenesis suggesting a considerable plasticity in the expression of glycolytic enzymes. Lactate dehydrogenase (LDH) isoenzymes, LDH-A and LDH-B, are expressed in premeiotic, meiotic cells, and early spermatids, both in avian and mammalian spermatogenesis. Highly polyadenylated forms, particularly of LDH-A, were detected in chicken testis. While mammals and columbid birds express the testis specific LDH-C gene in meiotic and postmeiotic cells, several LDH-B testis specific transcripts were detected in the corresponding cells during chicken spermatogenesis. These testis specific transcripts and the mRNA of mammalian LDH-C show several properties in common, such as temporal correlation of expression, mRNA stability, and repression of premature translation. These observations suggest that the testis specific transcripts could perform during chicken spermatogenesis the functions of the LDH-C mRNA in mammalian testis. (Less)
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organization
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Contribution to journal
publication status
published
subject
keywords
* 5' Untranslated Regions * Amino Acid Sequence * Animals * Base Sequence * Chickens * DNA Primers * Isoenzymes * L-Lactate Dehydrogenase/genetics* * Male * Molecular Sequence Data * RNA, Messenger/genetics* * Spermatogenesis* * Testis/enzymology*
in
Journal of Cellular Biochemistry
volume
79
issue
1
pages
15 - 27
publisher
Wiley-Blackwell
external identifiers
  • scopus:0033836360
ISSN
0730-2312
DOI
10.1095/​biolreprod64.2.507
language
English
LU publication?
yes
id
fc3d013c-cf1a-4248-983d-1c8d2c2c777f (old id 1748885)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/10906752
date added to LUP
2010-12-22 12:02:07
date last changed
2017-01-01 05:10:58
@article{fc3d013c-cf1a-4248-983d-1c8d2c2c777f,
  abstract     = {The substrates required for glycolysis change markedly at successive stages of spermatogenesis suggesting a considerable plasticity in the expression of glycolytic enzymes. Lactate dehydrogenase (LDH) isoenzymes, LDH-A and LDH-B, are expressed in premeiotic, meiotic cells, and early spermatids, both in avian and mammalian spermatogenesis. Highly polyadenylated forms, particularly of LDH-A, were detected in chicken testis. While mammals and columbid birds express the testis specific LDH-C gene in meiotic and postmeiotic cells, several LDH-B testis specific transcripts were detected in the corresponding cells during chicken spermatogenesis. These testis specific transcripts and the mRNA of mammalian LDH-C show several properties in common, such as temporal correlation of expression, mRNA stability, and repression of premature translation. These observations suggest that the testis specific transcripts could perform during chicken spermatogenesis the functions of the LDH-C mRNA in mammalian testis.},
  author       = {Martinez Arias, Wilma and Mezquita, C and Mezquita, J},
  issn         = {0730-2312},
  keyword      = {* 5' Untranslated Regions * Amino Acid Sequence * Animals * Base Sequence * Chickens * DNA Primers * Isoenzymes * L-Lactate Dehydrogenase/genetics* * Male * Molecular Sequence Data * RNA,Messenger/genetics* * Spermatogenesis* * Testis/enzymology*},
  language     = {eng},
  number       = {1},
  pages        = {15--27},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Cellular Biochemistry},
  title        = {Expression of lactate dehydrogenases A and B during chicken spermatogenesis: characterization of testis specific transcripts.},
  url          = {http://dx.doi.org/10.1095/​biolreprod64.2.507},
  volume       = {79},
  year         = {2000},
}