Advanced

Loss of low molecular weight dissolved organic carbon (DOC) and nitrogen (DON) in H2O and 0.5 M K2SO4 soil extracts

Rousk, Johannes LU and Jones, Davey L. (2010) In Soil Biology & Biochemistry 42(12). p.2331-2335
Abstract
Soil extracts are routinely used to quantify dissolved organic nutrient concentrations in soil. Here we studied the loss and transformation of low molecular weight (LMW) components of DOC (C-14-glucose, 1 and 100 mu M) and DON (C-14-amino acid mixture, 1 and 100 mu M) during extraction of soil (0-6 h) with either distilled water or 0.5 M K2SO4. The extractions were performed at 20 degrees C, at 4 degrees C, or in the presence of an inhibitor of microbial activity (HgCl2 and Na-azide). We showed that both glucose and amino acids became progressively lost from solution with increasing shaking time. The greatest loss was observed in H2O extracts at 1 mu M for both substances (>90% loss after 15 min). Lower temperature (4 degrees C) and... (More)
Soil extracts are routinely used to quantify dissolved organic nutrient concentrations in soil. Here we studied the loss and transformation of low molecular weight (LMW) components of DOC (C-14-glucose, 1 and 100 mu M) and DON (C-14-amino acid mixture, 1 and 100 mu M) during extraction of soil (0-6 h) with either distilled water or 0.5 M K2SO4. The extractions were performed at 20 degrees C, at 4 degrees C, or in the presence of an inhibitor of microbial activity (HgCl2 and Na-azide). We showed that both glucose and amino acids became progressively lost from solution with increasing shaking time. The greatest loss was observed in H2O extracts at 1 mu M for both substances (>90% loss after 15 min). Lower temperature (4 degrees C) and presence of K2SO4 both resulted in reduced loss rates. The presence of microbial inhibitors effectively eliminated the loss of glucose and amino acids. We conclude that microbial transformation of LMW-DOC and DON during H2O or K2SO4 extraction of soil may affect the estimation of their concentrations in soil. This finding has significant implications for methods that rely on chemical extractions to estimate LMW-C components of DOC and DON. (c) 2010 Elsevier Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Soil organic matter turnover, Mineralization, Microbial biomass, Method, Biodegradation, Decomposition, Glucose, Amino acids
in
Soil Biology & Biochemistry
volume
42
issue
12
pages
2331 - 2335
publisher
Elsevier
external identifiers
  • wos:000284294600034
  • scopus:78650192971
ISSN
0038-0717
DOI
10.1016/j.soilbio.2010.08.017
project
Carbon drivers and microbial agents of soil respiration
Microbial carbon-use efficiency
BECC
language
English
LU publication?
yes
id
3511f197-487b-4f50-8682-ad0744090b5a (old id 1752021)
date added to LUP
2010-12-29 15:00:52
date last changed
2018-06-17 04:24:51
@article{3511f197-487b-4f50-8682-ad0744090b5a,
  abstract     = {Soil extracts are routinely used to quantify dissolved organic nutrient concentrations in soil. Here we studied the loss and transformation of low molecular weight (LMW) components of DOC (C-14-glucose, 1 and 100 mu M) and DON (C-14-amino acid mixture, 1 and 100 mu M) during extraction of soil (0-6 h) with either distilled water or 0.5 M K2SO4. The extractions were performed at 20 degrees C, at 4 degrees C, or in the presence of an inhibitor of microbial activity (HgCl2 and Na-azide). We showed that both glucose and amino acids became progressively lost from solution with increasing shaking time. The greatest loss was observed in H2O extracts at 1 mu M for both substances (>90% loss after 15 min). Lower temperature (4 degrees C) and presence of K2SO4 both resulted in reduced loss rates. The presence of microbial inhibitors effectively eliminated the loss of glucose and amino acids. We conclude that microbial transformation of LMW-DOC and DON during H2O or K2SO4 extraction of soil may affect the estimation of their concentrations in soil. This finding has significant implications for methods that rely on chemical extractions to estimate LMW-C components of DOC and DON. (c) 2010 Elsevier Ltd. All rights reserved.},
  author       = {Rousk, Johannes and Jones, Davey L.},
  issn         = {0038-0717},
  keyword      = {Soil organic matter turnover,Mineralization,Microbial biomass,Method,Biodegradation,Decomposition,Glucose,Amino acids},
  language     = {eng},
  number       = {12},
  pages        = {2331--2335},
  publisher    = {Elsevier},
  series       = {Soil Biology & Biochemistry},
  title        = {Loss of low molecular weight dissolved organic carbon (DOC) and nitrogen (DON) in H2O and 0.5 M K2SO4 soil extracts},
  url          = {http://dx.doi.org/10.1016/j.soilbio.2010.08.017},
  volume       = {42},
  year         = {2010},
}