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Characterization of pre-analytical sample handling effects on a panel of Alzheimer's disease–related blood-based biomarkers : Results from the Standardization of Alzheimer's Blood Biomarkers (SABB) working group

Verberk, Inge M.W. ; Misdorp, Els O. ; Koelewijn, Jannet ; Ball, Andrew J. ; Blennow, Kaj LU ; Dage, Jeffrey L. ; Fandos, Noelia ; Hansson, Oskar LU orcid ; Hirtz, Christophe and Janelidze, Shorena LU , et al. (2022) In Alzheimer's and Dementia 18(8). p.1484-1497
Abstract

Introduction: Pre-analytical sample handling might affect the results of Alzheimer's disease blood-based biomarkers. We empirically tested variations of common blood collection and handling procedures. Methods: We created sample sets that address the effect of blood collection tube type, and of ethylene diamine tetraacetic acid plasma delayed centrifugation, centrifugation temperature, aliquot volume, delayed storage, and freeze–thawing. We measured amyloid beta (Aβ)42 and 40 peptides with six assays, and Aβ oligomerization-tendency (OAβ), amyloid precursor protein (APP)699-711, glial fibrillary acidic protein (GFAP), neurofilament light (NfL), total tau (t-tau), and phosphorylated tau181. Results: Collection tube type... (More)

Introduction: Pre-analytical sample handling might affect the results of Alzheimer's disease blood-based biomarkers. We empirically tested variations of common blood collection and handling procedures. Methods: We created sample sets that address the effect of blood collection tube type, and of ethylene diamine tetraacetic acid plasma delayed centrifugation, centrifugation temperature, aliquot volume, delayed storage, and freeze–thawing. We measured amyloid beta (Aβ)42 and 40 peptides with six assays, and Aβ oligomerization-tendency (OAβ), amyloid precursor protein (APP)699-711, glial fibrillary acidic protein (GFAP), neurofilament light (NfL), total tau (t-tau), and phosphorylated tau181. Results: Collection tube type resulted in different values of all assessed markers. Delayed plasma centrifugation and storage affected Aβ and t-tau; t-tau was additionally affected by centrifugation temperature. The other markers were resistant to handling variations. Discussion: We constructed a standardized operating procedure for plasma handling, to facilitate introduction of blood-based biomarkers into the research and clinical settings.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Alzheimer's disease, amyloid beta, plasma biomarkers, pre-analytics, stability, tau
in
Alzheimer's and Dementia
volume
18
issue
8
pages
1484 - 1497
publisher
Wiley
external identifiers
  • scopus:85120090019
  • pmid:34845818
ISSN
1552-5260
DOI
10.1002/alz.12510
language
English
LU publication?
yes
id
1752dd1e-8c86-401e-b912-d86f2d65e4f4
date added to LUP
2021-12-14 13:14:43
date last changed
2024-04-20 18:42:48
@article{1752dd1e-8c86-401e-b912-d86f2d65e4f4,
  abstract     = {{<p>Introduction: Pre-analytical sample handling might affect the results of Alzheimer's disease blood-based biomarkers. We empirically tested variations of common blood collection and handling procedures. Methods: We created sample sets that address the effect of blood collection tube type, and of ethylene diamine tetraacetic acid plasma delayed centrifugation, centrifugation temperature, aliquot volume, delayed storage, and freeze–thawing. We measured amyloid beta (Aβ)42 and 40 peptides with six assays, and Aβ oligomerization-tendency (OAβ), amyloid precursor protein (APP)<sub>699-711</sub>, glial fibrillary acidic protein (GFAP), neurofilament light (NfL), total tau (t-tau), and phosphorylated tau181. Results: Collection tube type resulted in different values of all assessed markers. Delayed plasma centrifugation and storage affected Aβ and t-tau; t-tau was additionally affected by centrifugation temperature. The other markers were resistant to handling variations. Discussion: We constructed a standardized operating procedure for plasma handling, to facilitate introduction of blood-based biomarkers into the research and clinical settings.</p>}},
  author       = {{Verberk, Inge M.W. and Misdorp, Els O. and Koelewijn, Jannet and Ball, Andrew J. and Blennow, Kaj and Dage, Jeffrey L. and Fandos, Noelia and Hansson, Oskar and Hirtz, Christophe and Janelidze, Shorena and Kang, Sungmin and Kirmess, Kristopher and Kindermans, Jana and Lee, Ryan and Meyer, Matthew R. and Shan, Dandan and Shaw, Leslie M. and Waligorska, Teresa and West, Tim and Zetterberg, Henrik and Edelmayer, Rebecca M. and Teunissen, Charlotte E.}},
  issn         = {{1552-5260}},
  keywords     = {{Alzheimer's disease; amyloid beta; plasma biomarkers; pre-analytics; stability; tau}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{1484--1497}},
  publisher    = {{Wiley}},
  series       = {{Alzheimer's and Dementia}},
  title        = {{Characterization of pre-analytical sample handling effects on a panel of Alzheimer's disease–related blood-based biomarkers : Results from the Standardization of Alzheimer's Blood Biomarkers (SABB) working group}},
  url          = {{http://dx.doi.org/10.1002/alz.12510}},
  doi          = {{10.1002/alz.12510}},
  volume       = {{18}},
  year         = {{2022}},
}