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Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: II. Performance of a laboratory network running the HPLC candidate reference measurement procedure and evaluation of a candidate reference material

Helander, Anders; Wielders, Jos P. M.; Jeppsson, Jan-Olof LU ; Weykamp, Cas; Siebelder, Carla; Anton, Raymond F.; Schellenberg, Francois and Whitfield, John B. (2010) In Clinical Chemistry and Laboratory Medicine 48(11). p.1585-1592
Abstract
Carbohydrate-deficient transferrin (CDT) is a descriptive term used for a temporary change in the transferrin glycosylation profile caused by alcohol, and used as a biomarker of chronic high alcohol consumption. The use of an array of methods for measurement of CDT in various absolute or relative amounts, and sometimes covering different transferrin glycoforms, has complicated the comparability of results and caused confusion among medical staff. This situation prompted initiation of an IFCC Working Group on CDT standardization. This second publication of the WG-CDT covers the establishment of a network of reference laboratories running a high-performance liquid chromatography (HPLC) candidate reference measurement procedure, and... (More)
Carbohydrate-deficient transferrin (CDT) is a descriptive term used for a temporary change in the transferrin glycosylation profile caused by alcohol, and used as a biomarker of chronic high alcohol consumption. The use of an array of methods for measurement of CDT in various absolute or relative amounts, and sometimes covering different transferrin glycoforms, has complicated the comparability of results and caused confusion among medical staff. This situation prompted initiation of an IFCC Working Group on CDT standardization. This second publication of the WG-CDT covers the establishment of a network of reference laboratories running a high-performance liquid chromatography (HPLC) candidate reference measurement procedure, and evaluation of candidate secondary reference materials. The network laboratories demonstrated good and reproducible performance and thus can be used to assign target values for calibrators and controls. A candidate secondary reference material based on native human serum lyophilized with a cryo-/lyoprotectant to prevent protein denaturation was found to be commutable and stable during storage. A proposed strategy for calibration of different CDT methods is also presented. In an external quality assurance study involving 66 laboratories and covering the current routine CDT assays (HPLC, capillary electrophoresis and immunoassay), recalculation of observed results based on the nominal values for the candidate calibrator reduced the overall coefficient of variation from 18.9% to 5.5%. The logistics for distribution of reference materials and review of results were found to be functional, indicating that a full reference system for CDT may soon be available. Clin Chem Lab Med 2010;48:1585-92. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
HPLC, disialotransferrin, alcohol biomarker, carbohydrate-deficient transferrin, standardization
in
Clinical Chemistry and Laboratory Medicine
volume
48
issue
11
pages
1585 - 1592
publisher
De Gruyter
external identifiers
  • wos:000284101000007
  • scopus:78751630075
ISSN
1434-6621
DOI
10.1515/CCLM.2010.322
language
English
LU publication?
yes
id
e19dd74c-a710-4472-8018-b96af7b43a4e (old id 1753388)
date added to LUP
2010-12-29 12:49:52
date last changed
2018-05-29 09:20:01
@article{e19dd74c-a710-4472-8018-b96af7b43a4e,
  abstract     = {Carbohydrate-deficient transferrin (CDT) is a descriptive term used for a temporary change in the transferrin glycosylation profile caused by alcohol, and used as a biomarker of chronic high alcohol consumption. The use of an array of methods for measurement of CDT in various absolute or relative amounts, and sometimes covering different transferrin glycoforms, has complicated the comparability of results and caused confusion among medical staff. This situation prompted initiation of an IFCC Working Group on CDT standardization. This second publication of the WG-CDT covers the establishment of a network of reference laboratories running a high-performance liquid chromatography (HPLC) candidate reference measurement procedure, and evaluation of candidate secondary reference materials. The network laboratories demonstrated good and reproducible performance and thus can be used to assign target values for calibrators and controls. A candidate secondary reference material based on native human serum lyophilized with a cryo-/lyoprotectant to prevent protein denaturation was found to be commutable and stable during storage. A proposed strategy for calibration of different CDT methods is also presented. In an external quality assurance study involving 66 laboratories and covering the current routine CDT assays (HPLC, capillary electrophoresis and immunoassay), recalculation of observed results based on the nominal values for the candidate calibrator reduced the overall coefficient of variation from 18.9% to 5.5%. The logistics for distribution of reference materials and review of results were found to be functional, indicating that a full reference system for CDT may soon be available. Clin Chem Lab Med 2010;48:1585-92.},
  author       = {Helander, Anders and Wielders, Jos P. M. and Jeppsson, Jan-Olof and Weykamp, Cas and Siebelder, Carla and Anton, Raymond F. and Schellenberg, Francois and Whitfield, John B.},
  issn         = {1434-6621},
  keyword      = {HPLC,disialotransferrin,alcohol biomarker,carbohydrate-deficient transferrin,standardization},
  language     = {eng},
  number       = {11},
  pages        = {1585--1592},
  publisher    = {De Gruyter},
  series       = {Clinical Chemistry and Laboratory Medicine},
  title        = {Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: II. Performance of a laboratory network running the HPLC candidate reference measurement procedure and evaluation of a candidate reference material},
  url          = {http://dx.doi.org/10.1515/CCLM.2010.322},
  volume       = {48},
  year         = {2010},
}