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Localized Deposition of Au Nanoparticles by Direct Electron Transfer through Cellobiose Dehydrogenase

Malel, Esteban; Ludwig, Roland; Gorton, Lo LU and Mandler, Daniel (2010) In Chemistry: A European Journal 16(38). p.11697-11706
Abstract
Cellobiose dehydrogenase (CDH) is a fascinating extracellular fungal enzyme that consists of two domains, one carrying a flavin adenine dinucleotide (FAD) and the other a cytochrome-type heme b group as cofactors. The two domains are interconnected by a linker and electrons can shuttle from the FAD to the heme group by intramolecular electron transfer. Electron transfer between CDH and an electrode can occur by direct electron transfer (DET) and by mediated electron transfer (MET). This characteristic makes CDH an interesting candidate for integration in systems such as biosensors and biofuel cells. Moreover, it makes CDH an alternative for the reduction of metal ions through DET and MET. In this work we have explored the localized... (More)
Cellobiose dehydrogenase (CDH) is a fascinating extracellular fungal enzyme that consists of two domains, one carrying a flavin adenine dinucleotide (FAD) and the other a cytochrome-type heme b group as cofactors. The two domains are interconnected by a linker and electrons can shuttle from the FAD to the heme group by intramolecular electron transfer. Electron transfer between CDH and an electrode can occur by direct electron transfer (DET) and by mediated electron transfer (MET). This characteristic makes CDH an interesting candidate for integration in systems such as biosensors and biofuel cells. Moreover, it makes CDH an alternative for the reduction of metal ions through DET and MET. In this work we have explored the localized deposition of gold on Pd substrates by CDH through DET and MET. For this purpose we exploited the advantage of scanning electrochemical microscopy (SECM) as a patterning tool. We first demonstrated that gold nanoparticles can be formed in homogenous solution. Then we showed that Au nanoparticles can also be locally formed and deposited on surfaces through DET at low pH and by MET at neutral pH using benzoquinone/hydroquinone as mediator. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
electrochemistry, biocatalysis, gold, enzymes, nanoparticies
in
Chemistry: A European Journal
volume
16
issue
38
pages
11697 - 11706
publisher
John Wiley & Sons
external identifiers
  • wos:000283634900026
  • scopus:77957588675
ISSN
1521-3765
DOI
10.1002/chem.201000453
language
English
LU publication?
yes
id
b6244939-74b2-4e8e-9b5b-f5c7f4eee4f1 (old id 1753410)
date added to LUP
2010-12-29 12:57:38
date last changed
2018-05-29 09:54:59
@article{b6244939-74b2-4e8e-9b5b-f5c7f4eee4f1,
  abstract     = {Cellobiose dehydrogenase (CDH) is a fascinating extracellular fungal enzyme that consists of two domains, one carrying a flavin adenine dinucleotide (FAD) and the other a cytochrome-type heme b group as cofactors. The two domains are interconnected by a linker and electrons can shuttle from the FAD to the heme group by intramolecular electron transfer. Electron transfer between CDH and an electrode can occur by direct electron transfer (DET) and by mediated electron transfer (MET). This characteristic makes CDH an interesting candidate for integration in systems such as biosensors and biofuel cells. Moreover, it makes CDH an alternative for the reduction of metal ions through DET and MET. In this work we have explored the localized deposition of gold on Pd substrates by CDH through DET and MET. For this purpose we exploited the advantage of scanning electrochemical microscopy (SECM) as a patterning tool. We first demonstrated that gold nanoparticles can be formed in homogenous solution. Then we showed that Au nanoparticles can also be locally formed and deposited on surfaces through DET at low pH and by MET at neutral pH using benzoquinone/hydroquinone as mediator.},
  author       = {Malel, Esteban and Ludwig, Roland and Gorton, Lo and Mandler, Daniel},
  issn         = {1521-3765},
  keyword      = {electrochemistry,biocatalysis,gold,enzymes,nanoparticies},
  language     = {eng},
  number       = {38},
  pages        = {11697--11706},
  publisher    = {John Wiley & Sons},
  series       = {Chemistry: A European Journal},
  title        = {Localized Deposition of Au Nanoparticles by Direct Electron Transfer through Cellobiose Dehydrogenase},
  url          = {http://dx.doi.org/10.1002/chem.201000453},
  volume       = {16},
  year         = {2010},
}