Localized Deposition of Au Nanoparticles by Direct Electron Transfer through Cellobiose Dehydrogenase
(2010) In Chemistry: A European Journal 16(38). p.11697-11706- Abstract
- Cellobiose dehydrogenase (CDH) is a fascinating extracellular fungal enzyme that consists of two domains, one carrying a flavin adenine dinucleotide (FAD) and the other a cytochrome-type heme b group as cofactors. The two domains are interconnected by a linker and electrons can shuttle from the FAD to the heme group by intramolecular electron transfer. Electron transfer between CDH and an electrode can occur by direct electron transfer (DET) and by mediated electron transfer (MET). This characteristic makes CDH an interesting candidate for integration in systems such as biosensors and biofuel cells. Moreover, it makes CDH an alternative for the reduction of metal ions through DET and MET. In this work we have explored the localized... (More)
- Cellobiose dehydrogenase (CDH) is a fascinating extracellular fungal enzyme that consists of two domains, one carrying a flavin adenine dinucleotide (FAD) and the other a cytochrome-type heme b group as cofactors. The two domains are interconnected by a linker and electrons can shuttle from the FAD to the heme group by intramolecular electron transfer. Electron transfer between CDH and an electrode can occur by direct electron transfer (DET) and by mediated electron transfer (MET). This characteristic makes CDH an interesting candidate for integration in systems such as biosensors and biofuel cells. Moreover, it makes CDH an alternative for the reduction of metal ions through DET and MET. In this work we have explored the localized deposition of gold on Pd substrates by CDH through DET and MET. For this purpose we exploited the advantage of scanning electrochemical microscopy (SECM) as a patterning tool. We first demonstrated that gold nanoparticles can be formed in homogenous solution. Then we showed that Au nanoparticles can also be locally formed and deposited on surfaces through DET at low pH and by MET at neutral pH using benzoquinone/hydroquinone as mediator. (Less)
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https://lup.lub.lu.se/record/1753410
- author
- Malel, Esteban ; Ludwig, Roland ; Gorton, Lo LU and Mandler, Daniel
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- electrochemistry, biocatalysis, gold, enzymes, nanoparticies
- in
- Chemistry: A European Journal
- volume
- 16
- issue
- 38
- pages
- 11697 - 11706
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000283634900026
- scopus:77957588675
- pmid:20821760
- ISSN
- 1521-3765
- DOI
- 10.1002/chem.201000453
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- b6244939-74b2-4e8e-9b5b-f5c7f4eee4f1 (old id 1753410)
- date added to LUP
- 2016-04-01 15:05:54
- date last changed
- 2023-09-03 23:34:43
@article{b6244939-74b2-4e8e-9b5b-f5c7f4eee4f1, abstract = {{Cellobiose dehydrogenase (CDH) is a fascinating extracellular fungal enzyme that consists of two domains, one carrying a flavin adenine dinucleotide (FAD) and the other a cytochrome-type heme b group as cofactors. The two domains are interconnected by a linker and electrons can shuttle from the FAD to the heme group by intramolecular electron transfer. Electron transfer between CDH and an electrode can occur by direct electron transfer (DET) and by mediated electron transfer (MET). This characteristic makes CDH an interesting candidate for integration in systems such as biosensors and biofuel cells. Moreover, it makes CDH an alternative for the reduction of metal ions through DET and MET. In this work we have explored the localized deposition of gold on Pd substrates by CDH through DET and MET. For this purpose we exploited the advantage of scanning electrochemical microscopy (SECM) as a patterning tool. We first demonstrated that gold nanoparticles can be formed in homogenous solution. Then we showed that Au nanoparticles can also be locally formed and deposited on surfaces through DET at low pH and by MET at neutral pH using benzoquinone/hydroquinone as mediator.}}, author = {{Malel, Esteban and Ludwig, Roland and Gorton, Lo and Mandler, Daniel}}, issn = {{1521-3765}}, keywords = {{electrochemistry; biocatalysis; gold; enzymes; nanoparticies}}, language = {{eng}}, number = {{38}}, pages = {{11697--11706}}, publisher = {{Wiley-Blackwell}}, series = {{Chemistry: A European Journal}}, title = {{Localized Deposition of Au Nanoparticles by Direct Electron Transfer through Cellobiose Dehydrogenase}}, url = {{http://dx.doi.org/10.1002/chem.201000453}}, doi = {{10.1002/chem.201000453}}, volume = {{16}}, year = {{2010}}, }