A Method to Study the Epigenetic Chromatin States of Rare Hematopoietic Stem and Progenitor Cells; MiniChIP-Chip
(2010) In Biological Procedures Online 12(1). p.1-17- Abstract
- Dynamic chromatin structure is a fundamental property of gene transcriptional regulation, and has emerged as a critical modulator of physiological processes during cellular differentiation and development. Analysis of chromatin structure using molecular biology and biochemical assays in rare somatic stem and progenitor cells is key for understanding these processes but poses a great challenge because of their reliance on millions of cells. Through the development of a miniaturized genome-scale chromatin immunoprecipitation method (miniChIP-chip), we have documented the genome-wide chromatin states of low abundant populations that comprise hematopoietic stem cells and immediate progeny residing in murine bone marrow. In this report, we... (More)
- Dynamic chromatin structure is a fundamental property of gene transcriptional regulation, and has emerged as a critical modulator of physiological processes during cellular differentiation and development. Analysis of chromatin structure using molecular biology and biochemical assays in rare somatic stem and progenitor cells is key for understanding these processes but poses a great challenge because of their reliance on millions of cells. Through the development of a miniaturized genome-scale chromatin immunoprecipitation method (miniChIP-chip), we have documented the genome-wide chromatin states of low abundant populations that comprise hematopoietic stem cells and immediate progeny residing in murine bone marrow. In this report, we describe the miniChIP methodology that can be used for increasing an understanding of the epigenetic mechanisms underlying hematopoietic stem and progenitor cell function. Application of this method will reveal the contribution of dynamic chromatin structure in regulating the function of other somatic stem cell populations, and how this process becomes perturbed in pathological conditions. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1753441
- author
- Weishaupt, Holger LU and Attema, Joanne LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Stem and progenitor cells, Histone modifications, technology, Miniaturized chromatin immunoprecipitation assays, Microarray, Epigenetic regulation, Lineage commitment
- in
- Biological Procedures Online
- volume
- 12
- issue
- 1
- pages
- 1 - 17
- publisher
- Springer
- external identifiers
-
- wos:000284155300001
- scopus:78649892923
- pmid:21406121
- ISSN
- 1480-9222
- DOI
- 10.1007/s12575-010-9031-y
- language
- English
- LU publication?
- yes
- id
- 52cd9a5d-fcfa-45d7-a89c-97e84c846671 (old id 1753441)
- date added to LUP
- 2016-04-01 14:51:00
- date last changed
- 2022-04-06 20:51:31
@article{52cd9a5d-fcfa-45d7-a89c-97e84c846671, abstract = {{Dynamic chromatin structure is a fundamental property of gene transcriptional regulation, and has emerged as a critical modulator of physiological processes during cellular differentiation and development. Analysis of chromatin structure using molecular biology and biochemical assays in rare somatic stem and progenitor cells is key for understanding these processes but poses a great challenge because of their reliance on millions of cells. Through the development of a miniaturized genome-scale chromatin immunoprecipitation method (miniChIP-chip), we have documented the genome-wide chromatin states of low abundant populations that comprise hematopoietic stem cells and immediate progeny residing in murine bone marrow. In this report, we describe the miniChIP methodology that can be used for increasing an understanding of the epigenetic mechanisms underlying hematopoietic stem and progenitor cell function. Application of this method will reveal the contribution of dynamic chromatin structure in regulating the function of other somatic stem cell populations, and how this process becomes perturbed in pathological conditions.}}, author = {{Weishaupt, Holger and Attema, Joanne}}, issn = {{1480-9222}}, keywords = {{Stem and progenitor cells; Histone modifications; technology; Miniaturized chromatin immunoprecipitation assays; Microarray; Epigenetic regulation; Lineage commitment}}, language = {{eng}}, number = {{1}}, pages = {{1--17}}, publisher = {{Springer}}, series = {{Biological Procedures Online}}, title = {{A Method to Study the Epigenetic Chromatin States of Rare Hematopoietic Stem and Progenitor Cells; MiniChIP-Chip}}, url = {{https://lup.lub.lu.se/search/files/4198472/1768612.pdf}}, doi = {{10.1007/s12575-010-9031-y}}, volume = {{12}}, year = {{2010}}, }