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A Method to Study the Epigenetic Chromatin States of Rare Hematopoietic Stem and Progenitor Cells; MiniChIP-Chip

Weishaupt, Holger LU and Attema, Joanne LU (2010) In Biological Procedures Online 12(1). p.1-17
Abstract
Dynamic chromatin structure is a fundamental property of gene transcriptional regulation, and has emerged as a critical modulator of physiological processes during cellular differentiation and development. Analysis of chromatin structure using molecular biology and biochemical assays in rare somatic stem and progenitor cells is key for understanding these processes but poses a great challenge because of their reliance on millions of cells. Through the development of a miniaturized genome-scale chromatin immunoprecipitation method (miniChIP-chip), we have documented the genome-wide chromatin states of low abundant populations that comprise hematopoietic stem cells and immediate progeny residing in murine bone marrow. In this report, we... (More)
Dynamic chromatin structure is a fundamental property of gene transcriptional regulation, and has emerged as a critical modulator of physiological processes during cellular differentiation and development. Analysis of chromatin structure using molecular biology and biochemical assays in rare somatic stem and progenitor cells is key for understanding these processes but poses a great challenge because of their reliance on millions of cells. Through the development of a miniaturized genome-scale chromatin immunoprecipitation method (miniChIP-chip), we have documented the genome-wide chromatin states of low abundant populations that comprise hematopoietic stem cells and immediate progeny residing in murine bone marrow. In this report, we describe the miniChIP methodology that can be used for increasing an understanding of the epigenetic mechanisms underlying hematopoietic stem and progenitor cell function. Application of this method will reveal the contribution of dynamic chromatin structure in regulating the function of other somatic stem cell populations, and how this process becomes perturbed in pathological conditions. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Stem and progenitor cells, Histone modifications, technology, Miniaturized chromatin immunoprecipitation assays, Microarray, Epigenetic regulation, Lineage commitment
in
Biological Procedures Online
volume
12
issue
1
pages
1 - 17
publisher
Springer
external identifiers
  • wos:000284155300001
  • scopus:78649892923
ISSN
1480-9222
DOI
10.1007/s12575-010-9031-y
language
English
LU publication?
yes
id
52cd9a5d-fcfa-45d7-a89c-97e84c846671 (old id 1753441)
date added to LUP
2011-01-04 08:14:52
date last changed
2018-06-24 04:26:24
@article{52cd9a5d-fcfa-45d7-a89c-97e84c846671,
  abstract     = {Dynamic chromatin structure is a fundamental property of gene transcriptional regulation, and has emerged as a critical modulator of physiological processes during cellular differentiation and development. Analysis of chromatin structure using molecular biology and biochemical assays in rare somatic stem and progenitor cells is key for understanding these processes but poses a great challenge because of their reliance on millions of cells. Through the development of a miniaturized genome-scale chromatin immunoprecipitation method (miniChIP-chip), we have documented the genome-wide chromatin states of low abundant populations that comprise hematopoietic stem cells and immediate progeny residing in murine bone marrow. In this report, we describe the miniChIP methodology that can be used for increasing an understanding of the epigenetic mechanisms underlying hematopoietic stem and progenitor cell function. Application of this method will reveal the contribution of dynamic chromatin structure in regulating the function of other somatic stem cell populations, and how this process becomes perturbed in pathological conditions.},
  author       = {Weishaupt, Holger and Attema, Joanne},
  issn         = {1480-9222},
  keyword      = {Stem and progenitor cells,Histone modifications,technology,Miniaturized chromatin immunoprecipitation assays,Microarray,Epigenetic regulation,Lineage commitment},
  language     = {eng},
  number       = {1},
  pages        = {1--17},
  publisher    = {Springer},
  series       = {Biological Procedures Online},
  title        = {A Method to Study the Epigenetic Chromatin States of Rare Hematopoietic Stem and Progenitor Cells; MiniChIP-Chip},
  url          = {http://dx.doi.org/10.1007/s12575-010-9031-y},
  volume       = {12},
  year         = {2010},
}