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Detection of Helicobacter rodentium-like DNA in the liver tissue of patients with chronic liver diseases by polymerase chain reaction-denaturing gradient gel electrophoresis and DNA sequence analysis

Nakonieczna, Joanna; Stalke, Piotr; Abu Al-Soud, Waleed LU ; Wadström, Torkel LU and Bielawski, Krzysztof Piotr (2010) In Diagnostic Microbiology and Infectious Disease 68(3). p.201-207
Abstract
Many Helicobacter spp. were isolated from the stomach, intestinal tract, and liver of different animals and humans. The association between Helicobacter spp. and hepatobiliary diseases, including hepatocellular carcinoma, was thoroughly examined, indicating a potential role of the bacteria in the progression toward cancer. In our work, we screened 97 liver biopsies from patients with chronic liver diseases for the presence of Helicobacter spp. DNA. With the use of genus-specific polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) and subsequent sequencing, we found that the majority of Helicobacter spp. DNA detected was similar to Helicobacter rodentium DNA (71%). The DNA of other detected Helicobacter spp. was... (More)
Many Helicobacter spp. were isolated from the stomach, intestinal tract, and liver of different animals and humans. The association between Helicobacter spp. and hepatobiliary diseases, including hepatocellular carcinoma, was thoroughly examined, indicating a potential role of the bacteria in the progression toward cancer. In our work, we screened 97 liver biopsies from patients with chronic liver diseases for the presence of Helicobacter spp. DNA. With the use of genus-specific polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) and subsequent sequencing, we found that the majority of Helicobacter spp. DNA detected was similar to Helicobacter rodentium DNA (71%). The DNA of other detected Helicobacter spp. was similar to Helicobacter pylori DNA. This is the first indication of H. rodentium-like DNA presence in human liver tissue. We also conclude that PCR DGGE is a useful screening method for assigning species designation and heterogeneity. (C) 2010 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
PCR-DGGE, Helicobacter, Chronic liver diseases
in
Diagnostic Microbiology and Infectious Disease
volume
68
issue
3
pages
201 - 207
publisher
Elsevier
external identifiers
  • wos:000283833100002
  • scopus:77957921061
ISSN
1879-0070
DOI
10.1016/j.diagmicrobio.2010.07.001
language
English
LU publication?
yes
id
59be65e0-e8b9-4ba8-af4a-b5d1926c96f1 (old id 1753626)
date added to LUP
2011-01-04 08:16:01
date last changed
2018-05-29 10:46:51
@article{59be65e0-e8b9-4ba8-af4a-b5d1926c96f1,
  abstract     = {Many Helicobacter spp. were isolated from the stomach, intestinal tract, and liver of different animals and humans. The association between Helicobacter spp. and hepatobiliary diseases, including hepatocellular carcinoma, was thoroughly examined, indicating a potential role of the bacteria in the progression toward cancer. In our work, we screened 97 liver biopsies from patients with chronic liver diseases for the presence of Helicobacter spp. DNA. With the use of genus-specific polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) and subsequent sequencing, we found that the majority of Helicobacter spp. DNA detected was similar to Helicobacter rodentium DNA (71%). The DNA of other detected Helicobacter spp. was similar to Helicobacter pylori DNA. This is the first indication of H. rodentium-like DNA presence in human liver tissue. We also conclude that PCR DGGE is a useful screening method for assigning species designation and heterogeneity. (C) 2010 Elsevier Inc. All rights reserved.},
  author       = {Nakonieczna, Joanna and Stalke, Piotr and Abu Al-Soud, Waleed and Wadström, Torkel and Bielawski, Krzysztof Piotr},
  issn         = {1879-0070},
  keyword      = {PCR-DGGE,Helicobacter,Chronic liver diseases},
  language     = {eng},
  number       = {3},
  pages        = {201--207},
  publisher    = {Elsevier},
  series       = {Diagnostic Microbiology and Infectious Disease},
  title        = {Detection of Helicobacter rodentium-like DNA in the liver tissue of patients with chronic liver diseases by polymerase chain reaction-denaturing gradient gel electrophoresis and DNA sequence analysis},
  url          = {http://dx.doi.org/10.1016/j.diagmicrobio.2010.07.001},
  volume       = {68},
  year         = {2010},
}