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Cannabinor, a Selective Cannabinoid-2 Receptor Agonist, Improves Bladder Emptying in Rats With Partial Urethral Obstruction.

Gratzke, Christian; Streng, Tomi; Stief, Christian G; Alroy, Iris; Limberg, Brian J; Downs, Thomas R; Rosenbaum, Jan S; Hedlund, Petter LU and Andersson, Karl-Erik LU (2011) In The Journal of urology 185. p.731-736
Abstract
PURPOSE:: We studied the effects of chronic treatment with the novel selective cannabinoid 2 receptor agonist cannabinor (Procter&Gamble Pharmaceuticals, Cincinnatti, Ohio) on bladder function in conscious rats with partial urethral obstruction and on the functional properties of isolated detrusor muscle. MATERIALS AND METHODS:: A total of 24 female Sprague-Dawley® rats with surgically created partial urethral obstruction received daily intraperitoneal injections of 3 mg/kg cannabinor (12) or saline as controls (12) for 2 weeks. Cystometry was done, the rats were sacrificed and the bladders were prepared for in vitro studies. RESULTS:: Mean ± SEM bladder weight was 0.97 ± 0.15 gm in controls and 0.53 ± 0.08 gm in cannabinor treated... (More)
PURPOSE:: We studied the effects of chronic treatment with the novel selective cannabinoid 2 receptor agonist cannabinor (Procter&Gamble Pharmaceuticals, Cincinnatti, Ohio) on bladder function in conscious rats with partial urethral obstruction and on the functional properties of isolated detrusor muscle. MATERIALS AND METHODS:: A total of 24 female Sprague-Dawley® rats with surgically created partial urethral obstruction received daily intraperitoneal injections of 3 mg/kg cannabinor (12) or saline as controls (12) for 2 weeks. Cystometry was done, the rats were sacrificed and the bladders were prepared for in vitro studies. RESULTS:: Mean ± SEM bladder weight was 0.97 ± 0.15 gm in controls and 0.53 ± 0.08 gm in cannabinor treated rats (p <0.05). There was no difference between the groups in the mean micturition interval, or mean baseline, threshold, flow or maximum pressure. In controls and cannabinor treated rats mean post-void residual volume was 0.28 ± 0.07 and 0.06 ± 0.02 ml, mean micturition compliance was 0.032 ± 0.006 and 0.069 ± 0.016 ml/cm H(2)O, and mean bladder wall force at the start of flow was 950 ± 280 and 1,647 ± 325 mN/gm, respectively (each p <0.05). Nonvoiding contractions were significantly less frequent in cannabinor treated rats than in controls. We noted no difference in carbachol (Sigma®) half maximum concentration between the groups but the carbachol maximum response in detrusor strips from cannabinor treated rats was significantly higher than that in control strips. CONCLUSIONS:: In rats with partial urethral obstruction treated daily for 14 days with cannabinor bladder weight was lower, the ability to empty the bladder was preserved and nonvoiding contraction frequency was low compared to those in controls. Detrusor preparations from cannabinor treated rats showed a higher response to nerve stimulation than those from controls. Selective cannabinoid 2 receptor activation may be a novel principle to enable improved bladder function after partial urethral obstruction. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The Journal of urology
volume
185
pages
731 - 736
publisher
Lippincott Williams & Wilkins
external identifiers
  • WOS:000286047500125
  • PMID:21168864
  • Scopus:78651304302
ISSN
1527-3792
DOI
10.1016/j.juro.2010.09.080
language
English
LU publication?
yes
id
4974c87e-66ac-4fa2-9e0e-0904dc25d7bc (old id 1756158)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21168864?dopt=Abstract
date added to LUP
2011-01-03 16:05:00
date last changed
2017-01-01 07:48:22
@article{4974c87e-66ac-4fa2-9e0e-0904dc25d7bc,
  abstract     = {PURPOSE:: We studied the effects of chronic treatment with the novel selective cannabinoid 2 receptor agonist cannabinor (Procter&amp;Gamble Pharmaceuticals, Cincinnatti, Ohio) on bladder function in conscious rats with partial urethral obstruction and on the functional properties of isolated detrusor muscle. MATERIALS AND METHODS:: A total of 24 female Sprague-Dawley® rats with surgically created partial urethral obstruction received daily intraperitoneal injections of 3 mg/kg cannabinor (12) or saline as controls (12) for 2 weeks. Cystometry was done, the rats were sacrificed and the bladders were prepared for in vitro studies. RESULTS:: Mean ± SEM bladder weight was 0.97 ± 0.15 gm in controls and 0.53 ± 0.08 gm in cannabinor treated rats (p &lt;0.05). There was no difference between the groups in the mean micturition interval, or mean baseline, threshold, flow or maximum pressure. In controls and cannabinor treated rats mean post-void residual volume was 0.28 ± 0.07 and 0.06 ± 0.02 ml, mean micturition compliance was 0.032 ± 0.006 and 0.069 ± 0.016 ml/cm H(2)O, and mean bladder wall force at the start of flow was 950 ± 280 and 1,647 ± 325 mN/gm, respectively (each p &lt;0.05). Nonvoiding contractions were significantly less frequent in cannabinor treated rats than in controls. We noted no difference in carbachol (Sigma®) half maximum concentration between the groups but the carbachol maximum response in detrusor strips from cannabinor treated rats was significantly higher than that in control strips. CONCLUSIONS:: In rats with partial urethral obstruction treated daily for 14 days with cannabinor bladder weight was lower, the ability to empty the bladder was preserved and nonvoiding contraction frequency was low compared to those in controls. Detrusor preparations from cannabinor treated rats showed a higher response to nerve stimulation than those from controls. Selective cannabinoid 2 receptor activation may be a novel principle to enable improved bladder function after partial urethral obstruction.},
  author       = {Gratzke, Christian and Streng, Tomi and Stief, Christian G and Alroy, Iris and Limberg, Brian J and Downs, Thomas R and Rosenbaum, Jan S and Hedlund, Petter and Andersson, Karl-Erik},
  issn         = {1527-3792},
  language     = {eng},
  pages        = {731--736},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {The Journal of urology},
  title        = {Cannabinor, a Selective Cannabinoid-2 Receptor Agonist, Improves Bladder Emptying in Rats With Partial Urethral Obstruction.},
  url          = {http://dx.doi.org/10.1016/j.juro.2010.09.080},
  volume       = {185},
  year         = {2011},
}