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Pulse Probiotic Administration Induces Repeated Small Intestinal Muc3 Expression in Rats.

Dykstra, Natalie S; Hyde, Lucie; Adawi, Diya LU ; Kulik, Dina; Ahrné, Siv; Molin, Göran; Jeppsson, Bengt LU ; Mackenzie, Alexander and Mack, David R (2011) In Pediatric Research 69. p.206-211
Abstract
Upon ingestion, probiotics may act to protect the host through a number of protective mechanisms including modulation of genes involved in intestinal innate mucosal defense such as epithelial cell derived mucin glycoproteins and inhibitor of apoptosis proteins. To determine the specificity of effect and sustainability of response in vivo, Lactobacillus plantarum 299v (Lp299v), Lactobacillus rhamnosus R0011 (LrR0011) and Bifidobacterium bifidum R0071 (BbR0071) were added repeatedly or intermittently to the drinking water of Sprague-Dawley rats. Following sacrifice via CO2 suffocation, Muc2, Muc3, NAIP, HIAP1/cIAP2 and HIAP2/cIAP1 mRNA and protein levels were analyzed via RT-PCR and immunohistochemistry. Live Lp299v, BbR0071 and LrR0011... (More)
Upon ingestion, probiotics may act to protect the host through a number of protective mechanisms including modulation of genes involved in intestinal innate mucosal defense such as epithelial cell derived mucin glycoproteins and inhibitor of apoptosis proteins. To determine the specificity of effect and sustainability of response in vivo, Lactobacillus plantarum 299v (Lp299v), Lactobacillus rhamnosus R0011 (LrR0011) and Bifidobacterium bifidum R0071 (BbR0071) were added repeatedly or intermittently to the drinking water of Sprague-Dawley rats. Following sacrifice via CO2 suffocation, Muc2, Muc3, NAIP, HIAP1/cIAP2 and HIAP2/cIAP1 mRNA and protein levels were analyzed via RT-PCR and immunohistochemistry. Live Lp299v, BbR0071 and LrR0011 increased Muc3 protein and mRNA expression in jejunum and ileum. Heat-killed and a non-adherent derivative of Lp299v failed to induce Muc3 expression. Lp299v did induce expression of HIAP2/cIAP1 and NAIP expression. Muc3 mucin expression was elevated for 5 days following oral administration of Lp299v but this effect was not sustained despite ongoing daily ingestion of a probiotic. Intermittent pulse ingestion of probiotics however, was found to repeatedly increase Muc3 expression. We conclude that selected probiotics can induce protective genes of mucosal intestinal epithelial cells, an effect that is reproducible with pulse probiotic administration. ABBREVIATIONS: (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Pediatric Research
volume
69
pages
206 - 211
publisher
International Pediatric Foundation Inc.
external identifiers
  • wos:000287621700005
  • pmid:21135754
  • scopus:79951609447
ISSN
1530-0447
DOI
10.1203/PDR.0b013e3182096ff0
language
English
LU publication?
yes
id
c9bb429a-78f6-453b-a3de-b82ebe019996 (old id 1756674)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21135754?dopt=Abstract
date added to LUP
2011-01-03 14:18:31
date last changed
2017-06-04 04:30:22
@article{c9bb429a-78f6-453b-a3de-b82ebe019996,
  abstract     = {Upon ingestion, probiotics may act to protect the host through a number of protective mechanisms including modulation of genes involved in intestinal innate mucosal defense such as epithelial cell derived mucin glycoproteins and inhibitor of apoptosis proteins. To determine the specificity of effect and sustainability of response in vivo, Lactobacillus plantarum 299v (Lp299v), Lactobacillus rhamnosus R0011 (LrR0011) and Bifidobacterium bifidum R0071 (BbR0071) were added repeatedly or intermittently to the drinking water of Sprague-Dawley rats. Following sacrifice via CO2 suffocation, Muc2, Muc3, NAIP, HIAP1/cIAP2 and HIAP2/cIAP1 mRNA and protein levels were analyzed via RT-PCR and immunohistochemistry. Live Lp299v, BbR0071 and LrR0011 increased Muc3 protein and mRNA expression in jejunum and ileum. Heat-killed and a non-adherent derivative of Lp299v failed to induce Muc3 expression. Lp299v did induce expression of HIAP2/cIAP1 and NAIP expression. Muc3 mucin expression was elevated for 5 days following oral administration of Lp299v but this effect was not sustained despite ongoing daily ingestion of a probiotic. Intermittent pulse ingestion of probiotics however, was found to repeatedly increase Muc3 expression. We conclude that selected probiotics can induce protective genes of mucosal intestinal epithelial cells, an effect that is reproducible with pulse probiotic administration. ABBREVIATIONS:},
  author       = {Dykstra, Natalie S and Hyde, Lucie and Adawi, Diya and Kulik, Dina and Ahrné, Siv and Molin, Göran and Jeppsson, Bengt and Mackenzie, Alexander and Mack, David R},
  issn         = {1530-0447},
  language     = {eng},
  pages        = {206--211},
  publisher    = {International Pediatric Foundation Inc.},
  series       = {Pediatric Research},
  title        = {Pulse Probiotic Administration Induces Repeated Small Intestinal Muc3 Expression in Rats.},
  url          = {http://dx.doi.org/10.1203/PDR.0b013e3182096ff0},
  volume       = {69},
  year         = {2011},
}