Detection of Alzheimer peptides and chemokines in the aqueous humor
(2011) In European Journal of Ophthalmology 21(1). p.104-111- Abstract
- PURPOSE. Alzheimer disease (AD) and age-related ocular diseases are characterized by inflammation and accumulation of insoluble proteins. We aimed to investigate the detectability and clinical relevance of a panel of AD-related markers, such as Alzheimer peptides and chemokines, in the aqueous humor (AH) samples taken from patients with cataract only, or cataract and 1 other ocular disease. METHODS. The AH samples were obtained during cataract surgery from patients with cataract only (n=162), cataract and glaucoma (n=21), cataract and exfoliation (PEX) (n=31), cataract and macular degeneration (n=36), and cataract and diabetic retinopathy (n=16). The AD peptides (A beta(1-42), A beta(1-40), A beta(1-38)) and chemokines (eotaxin, eotaxin 3,... (More)
- PURPOSE. Alzheimer disease (AD) and age-related ocular diseases are characterized by inflammation and accumulation of insoluble proteins. We aimed to investigate the detectability and clinical relevance of a panel of AD-related markers, such as Alzheimer peptides and chemokines, in the aqueous humor (AH) samples taken from patients with cataract only, or cataract and 1 other ocular disease. METHODS. The AH samples were obtained during cataract surgery from patients with cataract only (n=162), cataract and glaucoma (n=21), cataract and exfoliation (PEX) (n=31), cataract and macular degeneration (n=36), and cataract and diabetic retinopathy (n=16). The AD peptides (A beta(1-42), A beta(1-40), A beta(1-38)) and chemokines (eotaxin, eotaxin 3, interleukin [IL]-8, inducible protein-10, monocyte chemotactic protein [MCP]-1, MCP-4, macrophage-derived chemokine, macrophage inflammatory protein-1 beta, thymus and activation-egulated chemokine) were quantified by using multiplex immunoassays. RESULTS. The levels of the AH peptides (A beta(1-38), A beta(1-40), A beta(1-42)) did not differ between disease groups. Independently of disease group, the A beta(1-38) levels correlated with A beta(1-40) and A beta(1-42) (p<0.001, n=277). Notably, the ratio A beta(1-42) to A beta(1-38) differed between PEX and macular degeneration (mean 95% confidence interval [CI] =8.12 [11.3-3.99] vs 2.23 [2.67-0.52], p=0.003). Among chemokines examined, only MCP-1 and IL-8 were detected in about 90% to 46% of all analyzed (n=266) samples. Higher levels of AH IL-8 were found in the glaucoma group than in cataract only (p=0.011). Independently of disease group, a correlation was observed between AH MCP-1 and IL-8 (rho=0.275, p<0.001, n=266) and between MCP-1 and A beta(1-40) (rho=0.239, p<0.001, n=266). CONCLUSIONS. Our findings highlight pathologic similarities between AD and eye diseases, and show the potential of modern technologies to detect AD biomarkers in age-related eye diseases. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1774237
- author
- Janciauskiene, Sabina ; Westin, Karin ; Grip, Olof LU and Krakau, Torsten LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cataract, Alzheimer disease, A beta peptides, Age-related ocular diseases, Chemokines, Inflammation
- in
- European Journal of Ophthalmology
- volume
- 21
- issue
- 1
- pages
- 104 - 111
- publisher
- Wichtig Editore
- external identifiers
-
- wos:000284782200016
- scopus:78650164046
- ISSN
- 1120-6721
- DOI
- 10.5301/EJO.2010.2108
- language
- English
- LU publication?
- yes
- id
- 23c2f2f5-be46-423b-aee8-094a06393723 (old id 1774237)
- date added to LUP
- 2016-04-01 11:02:52
- date last changed
- 2022-02-02 23:20:07
@article{23c2f2f5-be46-423b-aee8-094a06393723, abstract = {{PURPOSE. Alzheimer disease (AD) and age-related ocular diseases are characterized by inflammation and accumulation of insoluble proteins. We aimed to investigate the detectability and clinical relevance of a panel of AD-related markers, such as Alzheimer peptides and chemokines, in the aqueous humor (AH) samples taken from patients with cataract only, or cataract and 1 other ocular disease. METHODS. The AH samples were obtained during cataract surgery from patients with cataract only (n=162), cataract and glaucoma (n=21), cataract and exfoliation (PEX) (n=31), cataract and macular degeneration (n=36), and cataract and diabetic retinopathy (n=16). The AD peptides (A beta(1-42), A beta(1-40), A beta(1-38)) and chemokines (eotaxin, eotaxin 3, interleukin [IL]-8, inducible protein-10, monocyte chemotactic protein [MCP]-1, MCP-4, macrophage-derived chemokine, macrophage inflammatory protein-1 beta, thymus and activation-egulated chemokine) were quantified by using multiplex immunoassays. RESULTS. The levels of the AH peptides (A beta(1-38), A beta(1-40), A beta(1-42)) did not differ between disease groups. Independently of disease group, the A beta(1-38) levels correlated with A beta(1-40) and A beta(1-42) (p<0.001, n=277). Notably, the ratio A beta(1-42) to A beta(1-38) differed between PEX and macular degeneration (mean 95% confidence interval [CI] =8.12 [11.3-3.99] vs 2.23 [2.67-0.52], p=0.003). Among chemokines examined, only MCP-1 and IL-8 were detected in about 90% to 46% of all analyzed (n=266) samples. Higher levels of AH IL-8 were found in the glaucoma group than in cataract only (p=0.011). Independently of disease group, a correlation was observed between AH MCP-1 and IL-8 (rho=0.275, p<0.001, n=266) and between MCP-1 and A beta(1-40) (rho=0.239, p<0.001, n=266). CONCLUSIONS. Our findings highlight pathologic similarities between AD and eye diseases, and show the potential of modern technologies to detect AD biomarkers in age-related eye diseases.}}, author = {{Janciauskiene, Sabina and Westin, Karin and Grip, Olof and Krakau, Torsten}}, issn = {{1120-6721}}, keywords = {{Cataract; Alzheimer disease; A beta peptides; Age-related ocular diseases; Chemokines; Inflammation}}, language = {{eng}}, number = {{1}}, pages = {{104--111}}, publisher = {{Wichtig Editore}}, series = {{European Journal of Ophthalmology}}, title = {{Detection of Alzheimer peptides and chemokines in the aqueous humor}}, url = {{http://dx.doi.org/10.5301/EJO.2010.2108}}, doi = {{10.5301/EJO.2010.2108}}, volume = {{21}}, year = {{2011}}, }