Advanced

Detection of Alzheimer peptides and chemokines in the aqueous humor

Janciauskiene, Sabina; Westin, Karin; Grip, Olof LU and Krakau, Torsten LU (2011) In European Journal of Ophthalmology 21(1). p.104-111
Abstract
PURPOSE. Alzheimer disease (AD) and age-related ocular diseases are characterized by inflammation and accumulation of insoluble proteins. We aimed to investigate the detectability and clinical relevance of a panel of AD-related markers, such as Alzheimer peptides and chemokines, in the aqueous humor (AH) samples taken from patients with cataract only, or cataract and 1 other ocular disease. METHODS. The AH samples were obtained during cataract surgery from patients with cataract only (n=162), cataract and glaucoma (n=21), cataract and exfoliation (PEX) (n=31), cataract and macular degeneration (n=36), and cataract and diabetic retinopathy (n=16). The AD peptides (A beta(1-42), A beta(1-40), A beta(1-38)) and chemokines (eotaxin, eotaxin 3,... (More)
PURPOSE. Alzheimer disease (AD) and age-related ocular diseases are characterized by inflammation and accumulation of insoluble proteins. We aimed to investigate the detectability and clinical relevance of a panel of AD-related markers, such as Alzheimer peptides and chemokines, in the aqueous humor (AH) samples taken from patients with cataract only, or cataract and 1 other ocular disease. METHODS. The AH samples were obtained during cataract surgery from patients with cataract only (n=162), cataract and glaucoma (n=21), cataract and exfoliation (PEX) (n=31), cataract and macular degeneration (n=36), and cataract and diabetic retinopathy (n=16). The AD peptides (A beta(1-42), A beta(1-40), A beta(1-38)) and chemokines (eotaxin, eotaxin 3, interleukin [IL]-8, inducible protein-10, monocyte chemotactic protein [MCP]-1, MCP-4, macrophage-derived chemokine, macrophage inflammatory protein-1 beta, thymus and activation-egulated chemokine) were quantified by using multiplex immunoassays. RESULTS. The levels of the AH peptides (A beta(1-38), A beta(1-40), A beta(1-42)) did not differ between disease groups. Independently of disease group, the A beta(1-38) levels correlated with A beta(1-40) and A beta(1-42) (p<0.001, n=277). Notably, the ratio A beta(1-42) to A beta(1-38) differed between PEX and macular degeneration (mean 95% confidence interval [CI] =8.12 [11.3-3.99] vs 2.23 [2.67-0.52], p=0.003). Among chemokines examined, only MCP-1 and IL-8 were detected in about 90% to 46% of all analyzed (n=266) samples. Higher levels of AH IL-8 were found in the glaucoma group than in cataract only (p=0.011). Independently of disease group, a correlation was observed between AH MCP-1 and IL-8 (rho=0.275, p<0.001, n=266) and between MCP-1 and A beta(1-40) (rho=0.239, p<0.001, n=266). CONCLUSIONS. Our findings highlight pathologic similarities between AD and eye diseases, and show the potential of modern technologies to detect AD biomarkers in age-related eye diseases. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cataract, Alzheimer disease, A beta peptides, Age-related ocular diseases, Chemokines, Inflammation
in
European Journal of Ophthalmology
volume
21
issue
1
pages
104 - 111
publisher
Wichtig Editore
external identifiers
  • wos:000284782200016
  • scopus:78650164046
ISSN
1120-6721
DOI
10.5301/EJO.2010.2108
language
English
LU publication?
yes
id
23c2f2f5-be46-423b-aee8-094a06393723 (old id 1774237)
date added to LUP
2011-02-01 09:57:16
date last changed
2017-06-18 03:25:23
@article{23c2f2f5-be46-423b-aee8-094a06393723,
  abstract     = {PURPOSE. Alzheimer disease (AD) and age-related ocular diseases are characterized by inflammation and accumulation of insoluble proteins. We aimed to investigate the detectability and clinical relevance of a panel of AD-related markers, such as Alzheimer peptides and chemokines, in the aqueous humor (AH) samples taken from patients with cataract only, or cataract and 1 other ocular disease. METHODS. The AH samples were obtained during cataract surgery from patients with cataract only (n=162), cataract and glaucoma (n=21), cataract and exfoliation (PEX) (n=31), cataract and macular degeneration (n=36), and cataract and diabetic retinopathy (n=16). The AD peptides (A beta(1-42), A beta(1-40), A beta(1-38)) and chemokines (eotaxin, eotaxin 3, interleukin [IL]-8, inducible protein-10, monocyte chemotactic protein [MCP]-1, MCP-4, macrophage-derived chemokine, macrophage inflammatory protein-1 beta, thymus and activation-egulated chemokine) were quantified by using multiplex immunoassays. RESULTS. The levels of the AH peptides (A beta(1-38), A beta(1-40), A beta(1-42)) did not differ between disease groups. Independently of disease group, the A beta(1-38) levels correlated with A beta(1-40) and A beta(1-42) (p&lt;0.001, n=277). Notably, the ratio A beta(1-42) to A beta(1-38) differed between PEX and macular degeneration (mean 95% confidence interval [CI] =8.12 [11.3-3.99] vs 2.23 [2.67-0.52], p=0.003). Among chemokines examined, only MCP-1 and IL-8 were detected in about 90% to 46% of all analyzed (n=266) samples. Higher levels of AH IL-8 were found in the glaucoma group than in cataract only (p=0.011). Independently of disease group, a correlation was observed between AH MCP-1 and IL-8 (rho=0.275, p&lt;0.001, n=266) and between MCP-1 and A beta(1-40) (rho=0.239, p&lt;0.001, n=266). CONCLUSIONS. Our findings highlight pathologic similarities between AD and eye diseases, and show the potential of modern technologies to detect AD biomarkers in age-related eye diseases.},
  author       = {Janciauskiene, Sabina and Westin, Karin and Grip, Olof and Krakau, Torsten},
  issn         = {1120-6721},
  keyword      = {Cataract,Alzheimer disease,A beta peptides,Age-related ocular diseases,Chemokines,Inflammation},
  language     = {eng},
  number       = {1},
  pages        = {104--111},
  publisher    = {Wichtig Editore},
  series       = {European Journal of Ophthalmology},
  title        = {Detection of Alzheimer peptides and chemokines in the aqueous humor},
  url          = {http://dx.doi.org/10.5301/EJO.2010.2108},
  volume       = {21},
  year         = {2011},
}