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Minimal residual disease assessment in childhood acute lymphoblastic leukaemia: a Swedish multi-centre study comparing real-time polymerase chain reaction and multicolour flow cytometry.

Thörnerup, Ingrid LU ; Forestier, Erik; Botling, Johan; Thuresson, Britt LU ; Wasslavik, Carina; Björklund, Elisabet; Li, Aihong; Lindström-Eriksson, Eleonor; Malec, Maria and Grönlund, Elisabeth, et al. (2011) In British Journal of Haematology 152(6). p.743-753
Abstract
Minimal residual disease (MRD) assessment is a powerful prognostic factor for determining the risk of relapse in childhood acute lymphoblastic leukaemia (ALL). In this Swedish multi-centre study of childhood ALL diagnosed between 2002 and 2006, the MRD levels were analysed in 726 follow-up samples in 228 children using real-time quantitative polymerase chain reaction (RQ-PCR) of rearranged immunoglobulin/T-cell receptor genes and multicolour flow cytometry (FCM). Using an MRD threshold of 0·1%, which was the sensitivity level reached in all analyses, the concordance between RQ-PCR and FCM MRD values at day 29 was 84%. In B-cell precursor ALL, an MRD level of ≥0·1% at day 29 predicted a higher risk of bone marrow relapse (BMR) with both... (More)
Minimal residual disease (MRD) assessment is a powerful prognostic factor for determining the risk of relapse in childhood acute lymphoblastic leukaemia (ALL). In this Swedish multi-centre study of childhood ALL diagnosed between 2002 and 2006, the MRD levels were analysed in 726 follow-up samples in 228 children using real-time quantitative polymerase chain reaction (RQ-PCR) of rearranged immunoglobulin/T-cell receptor genes and multicolour flow cytometry (FCM). Using an MRD threshold of 0·1%, which was the sensitivity level reached in all analyses, the concordance between RQ-PCR and FCM MRD values at day 29 was 84%. In B-cell precursor ALL, an MRD level of ≥0·1% at day 29 predicted a higher risk of bone marrow relapse (BMR) with both methods, although FCM was a better discriminator. However, considering the higher median MRD values achieved with RQ-PCR, a higher MRD cut-off (≥0·2%) improved the predictive capacity of RQ-PCR. In T-ALL, RQ-PCR was notably superior to FCM in predicting risk of BMR. That notwithstanding, MRD levels of ≥0·1%, detected by either method at day 29, could not predict isolated extramedullary relapse. In conclusion, the concordance between RQ-PCR and FCM was high and hence both methods are valuable clinical tools for identifying childhood ALL cases with increased risk of BMR. (Less)
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keywords
childhood acute lymphoblastic leukaemia, disease, minimal residual, TCR genes, rearranged IG, flow cytometry, RQ-PCR
in
British Journal of Haematology
volume
152
issue
6
pages
743 - 753
publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
external identifiers
  • wos:000287739500008
  • pmid:21250970
  • scopus:79952008832
ISSN
0007-1048
DOI
10.1111/j.1365-2141.2010.08456.x
language
English
LU publication?
yes
id
4ace96e1-6de7-45b8-87b3-1cbc2caf7293 (old id 1777237)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21250970?dopt=Abstract
date added to LUP
2011-02-01 15:21:33
date last changed
2017-10-29 03:23:20
@article{4ace96e1-6de7-45b8-87b3-1cbc2caf7293,
  abstract     = {Minimal residual disease (MRD) assessment is a powerful prognostic factor for determining the risk of relapse in childhood acute lymphoblastic leukaemia (ALL). In this Swedish multi-centre study of childhood ALL diagnosed between 2002 and 2006, the MRD levels were analysed in 726 follow-up samples in 228 children using real-time quantitative polymerase chain reaction (RQ-PCR) of rearranged immunoglobulin/T-cell receptor genes and multicolour flow cytometry (FCM). Using an MRD threshold of 0·1%, which was the sensitivity level reached in all analyses, the concordance between RQ-PCR and FCM MRD values at day 29 was 84%. In B-cell precursor ALL, an MRD level of ≥0·1% at day 29 predicted a higher risk of bone marrow relapse (BMR) with both methods, although FCM was a better discriminator. However, considering the higher median MRD values achieved with RQ-PCR, a higher MRD cut-off (≥0·2%) improved the predictive capacity of RQ-PCR. In T-ALL, RQ-PCR was notably superior to FCM in predicting risk of BMR. That notwithstanding, MRD levels of ≥0·1%, detected by either method at day 29, could not predict isolated extramedullary relapse. In conclusion, the concordance between RQ-PCR and FCM was high and hence both methods are valuable clinical tools for identifying childhood ALL cases with increased risk of BMR.},
  author       = {Thörnerup, Ingrid and Forestier, Erik and Botling, Johan and Thuresson, Britt and Wasslavik, Carina and Björklund, Elisabet and Li, Aihong and Lindström-Eriksson, Eleonor and Malec, Maria and Grönlund, Elisabeth and Torikka, Kerstin and Heldrup, Jesper and Abrahamsson, Jonas and Behrendtz, Mikael and Söderhäll, Stefan and Jacobsson, Stefan and Olofsson, Tor and Porwit, Anna and Lönnerholm, Gudmar and Rosenquist, Richard and Sundström, Christer},
  issn         = {0007-1048},
  keyword      = {childhood acute lymphoblastic leukaemia,disease,minimal residual,TCR genes,rearranged IG,flow cytometry,RQ-PCR},
  language     = {eng},
  number       = {6},
  pages        = {743--753},
  publisher    = {Federation of European Neuroscience Societies and Blackwell Publishing Ltd},
  series       = {British Journal of Haematology},
  title        = {Minimal residual disease assessment in childhood acute lymphoblastic leukaemia: a Swedish multi-centre study comparing real-time polymerase chain reaction and multicolour flow cytometry.},
  url          = {http://dx.doi.org/10.1111/j.1365-2141.2010.08456.x},
  volume       = {152},
  year         = {2011},
}