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Streamlined Preparation of Immobilized Candida antarctica Lipase B

Cassimjee, Karim Engelmark ; Hendil-Forssell, Peter ; Volkov, Alexey ; Krog, Anne ; Malmo, Jostein ; Aune, Trond Erik V ; Knecht, Wolfgang LU ; Miskelly, Iain R ; Moody, Thomas S and Svedendahl Humble, Maria (2017) In ACS Omega 2(12). p.8674-8677
Abstract

Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L-1) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catalyst with superior activity to Novozym 435.

Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
ACS Omega
volume
2
issue
12
pages
8674 - 8677
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:30023589
  • scopus:85059801499
ISSN
2470-1343
DOI
10.1021/acsomega.7b01510
language
English
LU publication?
yes
id
1781d522-9acd-41c5-bdb8-a93944c8bbb7
date added to LUP
2019-05-16 21:46:59
date last changed
2024-01-30 17:04:58
@article{1781d522-9acd-41c5-bdb8-a93944c8bbb7,
  abstract     = {{<p>Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L-1) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catalyst with superior activity to Novozym 435.</p>}},
  author       = {{Cassimjee, Karim Engelmark and Hendil-Forssell, Peter and Volkov, Alexey and Krog, Anne and Malmo, Jostein and Aune, Trond Erik V and Knecht, Wolfgang and Miskelly, Iain R and Moody, Thomas S and Svedendahl Humble, Maria}},
  issn         = {{2470-1343}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{12}},
  pages        = {{8674--8677}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{ACS Omega}},
  title        = {{Streamlined Preparation of Immobilized Candida antarctica Lipase B}},
  url          = {{http://dx.doi.org/10.1021/acsomega.7b01510}},
  doi          = {{10.1021/acsomega.7b01510}},
  volume       = {{2}},
  year         = {{2017}},
}