Streamlined Preparation of Immobilized Candida antarctica Lipase B
(2017) In ACS Omega 2(12). p.8674-8677- Abstract
Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L-1) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catalyst with superior activity to Novozym 435.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1781d522-9acd-41c5-bdb8-a93944c8bbb7
- author
- Cassimjee, Karim Engelmark ; Hendil-Forssell, Peter ; Volkov, Alexey ; Krog, Anne ; Malmo, Jostein ; Aune, Trond Erik V ; Knecht, Wolfgang LU ; Miskelly, Iain R ; Moody, Thomas S and Svedendahl Humble, Maria
- organization
- publishing date
- 2017-12-06
- type
- Contribution to journal
- publication status
- published
- subject
- in
- ACS Omega
- volume
- 2
- issue
- 12
- pages
- 8674 - 8677
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- pmid:30023589
- scopus:85059801499
- ISSN
- 2470-1343
- DOI
- 10.1021/acsomega.7b01510
- language
- English
- LU publication?
- yes
- id
- 1781d522-9acd-41c5-bdb8-a93944c8bbb7
- date added to LUP
- 2019-05-16 21:46:59
- date last changed
- 2024-09-18 20:23:46
@article{1781d522-9acd-41c5-bdb8-a93944c8bbb7, abstract = {{<p>Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L-1) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catalyst with superior activity to Novozym 435.</p>}}, author = {{Cassimjee, Karim Engelmark and Hendil-Forssell, Peter and Volkov, Alexey and Krog, Anne and Malmo, Jostein and Aune, Trond Erik V and Knecht, Wolfgang and Miskelly, Iain R and Moody, Thomas S and Svedendahl Humble, Maria}}, issn = {{2470-1343}}, language = {{eng}}, month = {{12}}, number = {{12}}, pages = {{8674--8677}}, publisher = {{The American Chemical Society (ACS)}}, series = {{ACS Omega}}, title = {{Streamlined Preparation of Immobilized Candida antarctica Lipase B}}, url = {{http://dx.doi.org/10.1021/acsomega.7b01510}}, doi = {{10.1021/acsomega.7b01510}}, volume = {{2}}, year = {{2017}}, }