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TNF-alpha suppresses the PDGF beta-receptor kinase

Molander, Catrin; Kallin, Anders; Izumi, H; Rönnstrand, Lars LU and Funa, Keiko (2000) In Experimental Cell Research 258(1). p.65-71
Abstract
PDGF and TNF-alpha are both known to play important roles in inflammation, albeit frequently by opposing actions. Typically, TNF-alpha can attenuate PDGF beta-receptor signaling. Pretreatment of mouse 3T3 L1 fibroblasts with TNF-alpha greatly diminished their proliferative response to PDGF. However, TNF-alpha affected neither the binding of PDGF-BB to cell surface receptors nor the total amount of PDGF beta-receptor in the cells, but decreased the PDGF-induced in vitro kinase activity of the receptor. The phosphatase inhibitor ortho-vanadate did not prevent this effect. Ortho-phosphate labeling of cells prior to TNF-alpha treatment and PDGF-BB stimulation confirmed a decrease of in vivo phosphorylation of the PDGF beta-receptor.... (More)
PDGF and TNF-alpha are both known to play important roles in inflammation, albeit frequently by opposing actions. Typically, TNF-alpha can attenuate PDGF beta-receptor signaling. Pretreatment of mouse 3T3 L1 fibroblasts with TNF-alpha greatly diminished their proliferative response to PDGF. However, TNF-alpha affected neither the binding of PDGF-BB to cell surface receptors nor the total amount of PDGF beta-receptor in the cells, but decreased the PDGF-induced in vitro kinase activity of the receptor. The phosphatase inhibitor ortho-vanadate did not prevent this effect. Ortho-phosphate labeling of cells prior to TNF-alpha treatment and PDGF-BB stimulation confirmed a decrease of in vivo phosphorylation of the PDGF beta-receptor. Two-dimensional mapping after tryptic cleavage as well as phosphoamino acid analysis demonstrated a general decrease in phosphorylation of all known tyrosine residues in the PDGF beta-receptor. The exact mechanism for this suppression remains to be clarified (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
3T3 Cells Animals Cell Division/drug effects Kinetics Mice Platelet-Derived Growth Factor/pharmacokinetics/*pharmacology Receptor Protein-Tyrosine Kinases/*antagonists & inhibitors Receptor, Platelet-Derived Growth Factor beta/drug effects/*physiology Signal Transduction/drug effects Transforming Growth Factor beta/*pharmacology Vanadates/pharmacology
in
Experimental Cell Research
volume
258
issue
1
pages
65 - 71
publisher
Academic Press
external identifiers
  • scopus:0034631829
ISSN
1090-2422
DOI
10.1006/excr.2000.4917
language
English
LU publication?
no
id
e08300d1-ec60-4d49-9b7e-91c4c00b11cf (old id 1782600)
date added to LUP
2011-02-07 17:44:29
date last changed
2017-01-01 07:38:28
@article{e08300d1-ec60-4d49-9b7e-91c4c00b11cf,
  abstract     = {PDGF and TNF-alpha are both known to play important roles in inflammation, albeit frequently by opposing actions. Typically, TNF-alpha can attenuate PDGF beta-receptor signaling. Pretreatment of mouse 3T3 L1 fibroblasts with TNF-alpha greatly diminished their proliferative response to PDGF. However, TNF-alpha affected neither the binding of PDGF-BB to cell surface receptors nor the total amount of PDGF beta-receptor in the cells, but decreased the PDGF-induced in vitro kinase activity of the receptor. The phosphatase inhibitor ortho-vanadate did not prevent this effect. Ortho-phosphate labeling of cells prior to TNF-alpha treatment and PDGF-BB stimulation confirmed a decrease of in vivo phosphorylation of the PDGF beta-receptor. Two-dimensional mapping after tryptic cleavage as well as phosphoamino acid analysis demonstrated a general decrease in phosphorylation of all known tyrosine residues in the PDGF beta-receptor. The exact mechanism for this suppression remains to be clarified},
  author       = {Molander, Catrin and Kallin, Anders and Izumi, H and Rönnstrand, Lars and Funa, Keiko},
  issn         = {1090-2422},
  keyword      = {3T3 Cells
Animals
Cell Division/drug effects
Kinetics
Mice
Platelet-Derived Growth Factor/pharmacokinetics/*pharmacology
Receptor Protein-Tyrosine Kinases/*antagonists & inhibitors
Receptor,Platelet-Derived Growth Factor beta/drug effects/*physiology
Signal Transduction/drug effects
Transforming Growth Factor beta/*pharmacology
Vanadates/pharmacology},
  language     = {eng},
  number       = {1},
  pages        = {65--71},
  publisher    = {Academic Press},
  series       = {Experimental Cell Research},
  title        = {TNF-alpha suppresses the PDGF beta-receptor kinase},
  url          = {http://dx.doi.org/10.1006/excr.2000.4917},
  volume       = {258},
  year         = {2000},
}