Identification of Tyr-703 and Tyr-936 as the primary association sites for Grb2 and Grb7 in the c-Kit/stem cell factor receptor
(1999) In Biochemical Journal 341(1). p.211-216- Abstract
- In this paper we demonstrate the presence of two novel in vivo autophosphorylation sites in the c-Kit/stem cell factor receptor (c-Kit/SCFR): Tyr-703 in the kinase insert and Tyr-936 in the C-terminal tail. We furthermore demonstrate that the adapter protein Grb2 is a specific binding partner for both phosphorylated Tyr-703 and phosphorylated Tyr-936, whereas the adapter protein Grb7 binds selectively to phosphorylated Tyr-936. It is shown that the association occurs through the Src homology 2 (SH2) domains of Grb2 and Grb7. Binding of Grb2 to Tyr-703 in the c-Kit/SCFR provides a link to the Ras/mitogen-activated protein kinase pathway.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1782630
- author
- Thömmes, Kerstin ; Lennartsson, Johan ; Carlberg, Monica and Rönnstrand, Lars LU
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Signal Transducing Cells, *Adaptor Proteins, Cultured Endothelium, Vascular/cytology GRB2 Adaptor Protein GRB7 Adaptor Protein Humans Phosphorylation Protein Binding Proteins/*metabolism Proto-Oncogene Proteins c-kit/genetics/*metabolism Receptor Protein-Tyrosine Kinases/genetics/metabolism Recombinant Fusion Proteins/metabolism Stem Cell Factor *Tyrosine src Homology Domains
- in
- Biochemical Journal
- volume
- 341
- issue
- 1
- pages
- 211 - 216
- publisher
- Portland Press
- external identifiers
-
- scopus:0037847347
- ISSN
- 0264-6021
- language
- English
- LU publication?
- no
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Experimental Clinical Chemistry (013016010)
- id
- fb278544-a1b3-4c75-a1b6-2cbd89e80e5b (old id 1782630)
- alternative location
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1220349/
- date added to LUP
- 2016-04-04 09:06:27
- date last changed
- 2022-01-29 08:17:27
@article{fb278544-a1b3-4c75-a1b6-2cbd89e80e5b, abstract = {{In this paper we demonstrate the presence of two novel in vivo autophosphorylation sites in the c-Kit/stem cell factor receptor (c-Kit/SCFR): Tyr-703 in the kinase insert and Tyr-936 in the C-terminal tail. We furthermore demonstrate that the adapter protein Grb2 is a specific binding partner for both phosphorylated Tyr-703 and phosphorylated Tyr-936, whereas the adapter protein Grb7 binds selectively to phosphorylated Tyr-936. It is shown that the association occurs through the Src homology 2 (SH2) domains of Grb2 and Grb7. Binding of Grb2 to Tyr-703 in the c-Kit/SCFR provides a link to the Ras/mitogen-activated protein kinase pathway.}}, author = {{Thömmes, Kerstin and Lennartsson, Johan and Carlberg, Monica and Rönnstrand, Lars}}, issn = {{0264-6021}}, keywords = {{Signal Transducing Cells; *Adaptor Proteins; Cultured Endothelium; Vascular/cytology GRB2 Adaptor Protein GRB7 Adaptor Protein Humans Phosphorylation Protein Binding Proteins/*metabolism Proto-Oncogene Proteins c-kit/genetics/*metabolism Receptor Protein-Tyrosine Kinases/genetics/metabolism Recombinant Fusion Proteins/metabolism Stem Cell Factor *Tyrosine src Homology Domains}}, language = {{eng}}, number = {{1}}, pages = {{211--216}}, publisher = {{Portland Press}}, series = {{Biochemical Journal}}, title = {{Identification of Tyr-703 and Tyr-936 as the primary association sites for Grb2 and Grb7 in the c-Kit/stem cell factor receptor}}, url = {{http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1220349/}}, volume = {{341}}, year = {{1999}}, }