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Identification of Tyr-703 and Tyr-936 as the primary association sites for Grb2 and Grb7 in the c-Kit/stem cell factor receptor

Thömmes, Kerstin; Lennartsson, Johan; Carlberg, Monica and Rönnstrand, Lars LU (1999) In Biochemical Journal 341(1). p.211-216
Abstract
In this paper we demonstrate the presence of two novel in vivo autophosphorylation sites in the c-Kit/stem cell factor receptor (c-Kit/SCFR): Tyr-703 in the kinase insert and Tyr-936 in the C-terminal tail. We furthermore demonstrate that the adapter protein Grb2 is a specific binding partner for both phosphorylated Tyr-703 and phosphorylated Tyr-936, whereas the adapter protein Grb7 binds selectively to phosphorylated Tyr-936. It is shown that the association occurs through the Src homology 2 (SH2) domains of Grb2 and Grb7. Binding of Grb2 to Tyr-703 in the c-Kit/SCFR provides a link to the Ras/mitogen-activated protein kinase pathway.
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Signal Transducing Cells, *Adaptor Proteins, Cultured Endothelium, Vascular/cytology GRB2 Adaptor Protein GRB7 Adaptor Protein Humans Phosphorylation Protein Binding Proteins/*metabolism Proto-Oncogene Proteins c-kit/genetics/*metabolism Receptor Protein-Tyrosine Kinases/genetics/metabolism Recombinant Fusion Proteins/metabolism Stem Cell Factor *Tyrosine src Homology Domains
in
Biochemical Journal
volume
341
issue
1
pages
211 - 216
publisher
Portland Press
external identifiers
  • Scopus:0037847347
ISSN
0264-6021
language
English
LU publication?
no
id
fb278544-a1b3-4c75-a1b6-2cbd89e80e5b (old id 1782630)
alternative location
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1220349/
date added to LUP
2011-02-07 17:38:11
date last changed
2017-01-01 07:42:39
@article{fb278544-a1b3-4c75-a1b6-2cbd89e80e5b,
  abstract     = {In this paper we demonstrate the presence of two novel in vivo autophosphorylation sites in the c-Kit/stem cell factor receptor (c-Kit/SCFR): Tyr-703 in the kinase insert and Tyr-936 in the C-terminal tail. We furthermore demonstrate that the adapter protein Grb2 is a specific binding partner for both phosphorylated Tyr-703 and phosphorylated Tyr-936, whereas the adapter protein Grb7 binds selectively to phosphorylated Tyr-936. It is shown that the association occurs through the Src homology 2 (SH2) domains of Grb2 and Grb7. Binding of Grb2 to Tyr-703 in the c-Kit/SCFR provides a link to the Ras/mitogen-activated protein kinase pathway.},
  author       = {Thömmes, Kerstin and Lennartsson, Johan and Carlberg, Monica and Rönnstrand, Lars},
  issn         = {0264-6021},
  keyword      = {Signal Transducing
Cells,*Adaptor Proteins,Cultured
Endothelium,Vascular/cytology
GRB2 Adaptor Protein
GRB7 Adaptor Protein
Humans
Phosphorylation
Protein Binding
Proteins/*metabolism
Proto-Oncogene Proteins c-kit/genetics/*metabolism
Receptor Protein-Tyrosine Kinases/genetics/metabolism
Recombinant Fusion Proteins/metabolism
Stem Cell Factor
*Tyrosine
src Homology Domains},
  language     = {eng},
  number       = {1},
  pages        = {211--216},
  publisher    = {Portland Press},
  series       = {Biochemical Journal},
  title        = {Identification of Tyr-703 and Tyr-936 as the primary association sites for Grb2 and Grb7 in the c-Kit/stem cell factor receptor},
  volume       = {341},
  year         = {1999},
}