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Temperature Induced Phase Transition of Encapsidated Phage DNA

Jeembaeva, Meerim LU ; Sae-Ueng, Udom; Nurmemmedov, Elmar LU ; Castelnovo, Martin; Jönsson, Bengt and Evilevitch, Alex LU (2011)
Abstract
Double-stranded DNA bacteriophages are highly stressed under the high internal pressure that is described as stored energy. Most of them eject their genome into the host bacteria via receptor binding. In the previous work, isothermal titration calorimtery (ITC) was used, for first time, to directly measure the heat released during DNA ejection from the phage lambda, triggered in solution by solubilized LamB receptor. In the current

study, using ITC, we investigate the ejection energy from the capsid of phage lambda packaged with 94-110% genome, at a wider temperature range between 18 and 40°C. We experimentally show that DNA undergoes phase transition when exposed to different temperatures. The event of phase transition was also... (More)
Double-stranded DNA bacteriophages are highly stressed under the high internal pressure that is described as stored energy. Most of them eject their genome into the host bacteria via receptor binding. In the previous work, isothermal titration calorimtery (ITC) was used, for first time, to directly measure the heat released during DNA ejection from the phage lambda, triggered in solution by solubilized LamB receptor. In the current

study, using ITC, we investigate the ejection energy from the capsid of phage lambda packaged with 94-110% genome, at a wider temperature range between 18 and 40°C. We experimentally show that DNA undergoes phase transition when exposed to different temperatures. The event of phase transition was also confirmed by differential scanning

calorimetry (DSC). (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Working Paper
publication status
unpublished
subject
keywords
isothermal titration calorimetry, enthalpy, DNA ejection, phage, differential scanning calorimetry
language
English
LU publication?
yes
id
53a48485-5723-4539-b359-9c8aa72af3ab (old id 1786291)
date added to LUP
2011-02-16 12:05:03
date last changed
2016-04-16 11:26:11
@misc{53a48485-5723-4539-b359-9c8aa72af3ab,
  abstract     = {Double-stranded DNA bacteriophages are highly stressed under the high internal pressure that is described as stored energy. Most of them eject their genome into the host bacteria via receptor binding. In the previous work, isothermal titration calorimtery (ITC) was used, for first time, to directly measure the heat released during DNA ejection from the phage lambda, triggered in solution by solubilized LamB receptor. In the current<br/><br>
study, using ITC, we investigate the ejection energy from the capsid of phage lambda packaged with 94-110% genome, at a wider temperature range between 18 and 40°C. We experimentally show that DNA undergoes phase transition when exposed to different temperatures. The event of phase transition was also confirmed by differential scanning<br/><br>
calorimetry (DSC).},
  author       = {Jeembaeva, Meerim and Sae-Ueng, Udom and Nurmemmedov, Elmar and Castelnovo, Martin and Jönsson, Bengt and Evilevitch, Alex},
  keyword      = {isothermal titration calorimetry,enthalpy,DNA ejection,phage,differential scanning calorimetry},
  language     = {eng},
  note         = {Working Paper},
  title        = {Temperature Induced Phase Transition of Encapsidated Phage DNA},
  year         = {2011},
}