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Dosage Compensation by Gene-Copy Silencing in a Triploid Hybrid Fish

Pala, Irene LU ; Coelho, Maria Manuela and Schartl, Manfred (2008) In Current Biology 18(17). p.1344-1348
Abstract
In mammals, the increase in gene dosage, in the form of polyploidy or involving chromosomal fragments, has deleterious effects [1]. Regulation of appropriate gene product amounts has to be warranted by complex dosage-compensation mechanisms. Lower vertebrates, on the other hand, cope very well with ploidy increase [2 4], implying either effective compensation or a lack of necessity for such mechanisms. Unfortunately, nothing is known about the genetic and molecular mechanisms underlying this phenomenon. For an experimental approach, we have studied gene expression in the allotriploid form of Squalius alburnoides. In these organisms, different genomes are joined through hybridization; thus, sequence differences can be used to follow... (More)
In mammals, the increase in gene dosage, in the form of polyploidy or involving chromosomal fragments, has deleterious effects [1]. Regulation of appropriate gene product amounts has to be warranted by complex dosage-compensation mechanisms. Lower vertebrates, on the other hand, cope very well with ploidy increase [2 4], implying either effective compensation or a lack of necessity for such mechanisms. Unfortunately, nothing is known about the genetic and molecular mechanisms underlying this phenomenon. For an experimental approach, we have studied gene expression in the allotriploid form of Squalius alburnoides. In these organisms, different genomes are joined through hybridization; thus, sequence differences can be used to follow expression of different alleles [5, 6]. We found that a compensation mechanism exists, reducing transcript levels to the diploid state. Our data suggest a silencing of one of the three alleles. Unexpectedly, it is not a whole haplome that is inactivated. The allelic expression patterns differ between genes and between different tissues for one and the same gene. Our data provide the first evidence of a regulation mechanism involving gene-copy silencing in a triploid vertebrate. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
DEVBIO
in
Current Biology
volume
18
issue
17
pages
1344 - 1348
publisher
Elsevier
external identifiers
  • scopus:50849097543
ISSN
1879-0445
DOI
10.1016/j.cub.2008.07.096
language
English
LU publication?
no
id
ed4c9ff9-4f38-40a3-8d6e-08a2e8bf1661 (old id 1787916)
date added to LUP
2011-02-22 09:43:09
date last changed
2017-11-05 03:38:29
@article{ed4c9ff9-4f38-40a3-8d6e-08a2e8bf1661,
  abstract     = {In mammals, the increase in gene dosage, in the form of polyploidy or involving chromosomal fragments, has deleterious effects [1]. Regulation of appropriate gene product amounts has to be warranted by complex dosage-compensation mechanisms. Lower vertebrates, on the other hand, cope very well with ploidy increase [2 4], implying either effective compensation or a lack of necessity for such mechanisms. Unfortunately, nothing is known about the genetic and molecular mechanisms underlying this phenomenon. For an experimental approach, we have studied gene expression in the allotriploid form of Squalius alburnoides. In these organisms, different genomes are joined through hybridization; thus, sequence differences can be used to follow expression of different alleles [5, 6]. We found that a compensation mechanism exists, reducing transcript levels to the diploid state. Our data suggest a silencing of one of the three alleles. Unexpectedly, it is not a whole haplome that is inactivated. The allelic expression patterns differ between genes and between different tissues for one and the same gene. Our data provide the first evidence of a regulation mechanism involving gene-copy silencing in a triploid vertebrate.},
  author       = {Pala, Irene and Coelho, Maria Manuela and Schartl, Manfred},
  issn         = {1879-0445},
  keyword      = {DEVBIO},
  language     = {eng},
  number       = {17},
  pages        = {1344--1348},
  publisher    = {Elsevier},
  series       = {Current Biology},
  title        = {Dosage Compensation by Gene-Copy Silencing in a Triploid Hybrid Fish},
  url          = {http://dx.doi.org/10.1016/j.cub.2008.07.096},
  volume       = {18},
  year         = {2008},
}