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Feasibility Study of Gel Filtration as a Separation Method for Blood Serum Proteins in Combination with PIXE Analysis

Pallon, Jan LU ; Pakarinen, Pirjo; Malmqvist, Klas LU and Akselsson, Roland LU (1987) In Biological Trace Element Research 12(1). p.401-409
Abstract
The combination between a protein separation technique and the PIXE method has a great potential for large surveys, including thousands of samples, in which multielemental analysis is required. Gel filtration with a Sephadex G-200 gel and a TRIS-acetate buffer was used for separating proteins in human serum. The fractions were doped with an yttrium/vanadium standard and then concentrated and pipeted onto Kimfol™ backing foils. Using the PIXE technique, the distributions of Fe, Cu, Zn, and Se, with respect to molecular size, were found, indicating binding to specific proteins. Sulfur and phosphorus were found to correlate well with the protein content measured by UV-absorption at 280 nm. Further developments and tests on the protein... (More)
The combination between a protein separation technique and the PIXE method has a great potential for large surveys, including thousands of samples, in which multielemental analysis is required. Gel filtration with a Sephadex G-200 gel and a TRIS-acetate buffer was used for separating proteins in human serum. The fractions were doped with an yttrium/vanadium standard and then concentrated and pipeted onto Kimfol™ backing foils. Using the PIXE technique, the distributions of Fe, Cu, Zn, and Se, with respect to molecular size, were found, indicating binding to specific proteins. Sulfur and phosphorus were found to correlate well with the protein content measured by UV-absorption at 280 nm. Further developments and tests on the protein separation technique is required, taking restrictions imposed by the PIXE method into consideration. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
metal-binding proteins, trace elements, protein separation, PIXE, work environmental health, human serum
in
Biological Trace Element Research
volume
12
issue
1
pages
401 - 409
publisher
Humana Press
external identifiers
  • scopus:51249174426
ISSN
1559-0720
DOI
10.1007/BF02796696
language
English
LU publication?
yes
id
f821f2e5-849c-44ef-8567-6527ed6ef203 (old id 1788662)
date added to LUP
2011-02-28 14:16:51
date last changed
2017-01-01 04:49:44
@article{f821f2e5-849c-44ef-8567-6527ed6ef203,
  abstract     = {The combination between a protein separation technique and the PIXE method has a great potential for large surveys, including thousands of samples, in which multielemental analysis is required. Gel filtration with a Sephadex G-200 gel and a TRIS-acetate buffer was used for separating proteins in human serum. The fractions were doped with an yttrium/vanadium standard and then concentrated and pipeted onto Kimfol™ backing foils. Using the PIXE technique, the distributions of Fe, Cu, Zn, and Se, with respect to molecular size, were found, indicating binding to specific proteins. Sulfur and phosphorus were found to correlate well with the protein content measured by UV-absorption at 280 nm. Further developments and tests on the protein separation technique is required, taking restrictions imposed by the PIXE method into consideration.},
  author       = {Pallon, Jan and Pakarinen, Pirjo and Malmqvist, Klas and Akselsson, Roland},
  issn         = {1559-0720},
  keyword      = {metal-binding proteins,trace elements,protein separation,PIXE,work environmental health,human serum},
  language     = {eng},
  number       = {1},
  pages        = {401--409},
  publisher    = {Humana Press},
  series       = {Biological Trace Element Research},
  title        = {Feasibility Study of Gel Filtration as a Separation Method for Blood Serum Proteins in Combination with PIXE Analysis},
  url          = {http://dx.doi.org/10.1007/BF02796696},
  volume       = {12},
  year         = {1987},
}