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Ten polymorphic microsatellite markers for Hieracium s.s. (Asteraceae)

Jönsson, Jane LU ; Wellenreuther, Maren LU and Tyler, Torbjörn LU (2010) In Conservation Genetics Resources 2(1). p.295-300
Abstract
Eight novel microsatellite markers were developed for Hieracium s.s. (excluding Pilosella) using a modified FIASCO protocol, and two out of nine microsatellites that were originally developed for Pilosella officinarum were optimised for Hieracium s.s.. All ten microsatellites consistently amplified loci from a wide range of representatives from north and central European species of various sections of the genus. When tested on 19 individuals, nine microsatellites were found to be highly polymorphic with 6–29 alleles, while one microsatellite marker was monomorphic. Cross-amplification showed that six of the ten microsatellites also amplified products in one taxon of Pilosella, P. floribunda × officinarum.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
microsatellite marker, FIASCO, Hieracium, Pilosella, genetic variation, cross-amplification
in
Conservation Genetics Resources
volume
2
issue
1
pages
295 - 300
publisher
Springer
external identifiers
  • wos:000290413000070
  • scopus:84860014720
ISSN
1877-7260
DOI
10.1007/s12686-010-9209-2
language
English
LU publication?
yes
id
1f1cb793-6499-4de7-aba7-6619453d2e7a (old id 1790444)
date added to LUP
2011-03-02 13:24:46
date last changed
2018-05-29 11:44:54
@article{1f1cb793-6499-4de7-aba7-6619453d2e7a,
  abstract     = {Eight novel microsatellite markers were developed for Hieracium s.s. (excluding Pilosella) using a modified FIASCO protocol, and two out of nine microsatellites that were originally developed for Pilosella officinarum were optimised for Hieracium s.s.. All ten microsatellites consistently amplified loci from a wide range of representatives from north and central European species of various sections of the genus. When tested on 19 individuals, nine microsatellites were found to be highly polymorphic with 6–29 alleles, while one microsatellite marker was monomorphic. Cross-amplification showed that six of the ten microsatellites also amplified products in one taxon of Pilosella, P. floribunda × officinarum.},
  author       = {Jönsson, Jane and Wellenreuther, Maren and Tyler, Torbjörn},
  issn         = {1877-7260},
  keyword      = {microsatellite marker,FIASCO,Hieracium,Pilosella,genetic variation,cross-amplification},
  language     = {eng},
  number       = {1},
  pages        = {295--300},
  publisher    = {Springer},
  series       = {Conservation Genetics Resources},
  title        = {Ten polymorphic microsatellite markers for Hieracium s.s. (Asteraceae)},
  url          = {http://dx.doi.org/10.1007/s12686-010-9209-2},
  volume       = {2},
  year         = {2010},
}