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Variation in allergen content over time of acrylates/methacrylates in patch test preparations

Goon, Anthony LU ; Bruze, Magnus LU ; Zimerson, Erik LU ; Sörensen, Östen LU ; Goh, C. L.; Koh, D. S. Q. and Isaksson, Marléne LU (2011) In British Journal of Dermatology 164(1). p.116-124
Abstract
P>Background Acrylates/methacrylates are volatile substances. There might be a gradual decrease in acrylate/methacrylate allergen content over time in patch test preparations but this has not yet been documented. Objectives To determine the allergen content of acrylates/methacrylates in patch test preparations over time under different storage conditions. Methods Five acrylate/methacrylate allergens [2-hydroxyethyl methacrylate (2-HEMA), methyl methacrylate (MMA), ethylene glycol dimethacrylate (EGDMA), triethylene glycol diacrylate (TREGDA) and 2-hydroxypropyl acrylate (2-HPA)] in syringes and IQ (TM) chambers (Chemotechnique Diagnostics, Vellinge, Sweden) were analysed using gel permeation chromatography and high-performance liquid... (More)
P>Background Acrylates/methacrylates are volatile substances. There might be a gradual decrease in acrylate/methacrylate allergen content over time in patch test preparations but this has not yet been documented. Objectives To determine the allergen content of acrylates/methacrylates in patch test preparations over time under different storage conditions. Methods Five acrylate/methacrylate allergens [2-hydroxyethyl methacrylate (2-HEMA), methyl methacrylate (MMA), ethylene glycol dimethacrylate (EGDMA), triethylene glycol diacrylate (TREGDA) and 2-hydroxypropyl acrylate (2-HPA)] in syringes and IQ (TM) chambers (Chemotechnique Diagnostics, Vellinge, Sweden) were analysed using gel permeation chromatography and high-performance liquid chromatography to measure the allergen content over time in samples stored in the freezer, refrigerator and under room temperature. Results The concentration of allergens in syringes decreased with time. Those stored at room temperature had the fastest rate of decrease, followed by those in the refrigerator and freezer. In most cases, in syringes or IQ (TM) chambers under all storage conditions, the MMA decreased most rapidly, followed by 2-HPA, 2-HEMA, EGDMA and TREGDA. The allergens in the IQ (TM) chambers rapidly disappeared, with almost all samples reaching nondetectable levels by day 8. MMA was the first to reach a nondetectable level - at day 2. Conclusions Acrylate/methacrylate allergens are lost rapidly from IQ (TM) chambers especially if stored at room temperature. Allergens in syringes remain above 80% of their initial concentrations for longer periods compared with IQ (TM) chambers. In syringes and IQ (TM) chambers there is a slower rate of decrease in concentration when the storage temperature is lower. Allergens should be stored refrigerated, replaced regularly, and freshly applied on to test patches on the day of use. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
British Journal of Dermatology
volume
164
issue
1
pages
116 - 124
publisher
Wiley-Blackwell
external identifiers
  • wos:000285752000018
  • pmid:21039407
  • scopus:78650483877
ISSN
1365-2133
DOI
10.1111/j.1365-2133.2010.10087.x
language
English
LU publication?
yes
id
575c4a3c-75fd-45b4-8d7e-a4d55636e25c (old id 1790963)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21039407?dopt=Abstract
date added to LUP
2011-03-02 14:31:48
date last changed
2017-10-22 03:29:59
@article{575c4a3c-75fd-45b4-8d7e-a4d55636e25c,
  abstract     = {P>Background Acrylates/methacrylates are volatile substances. There might be a gradual decrease in acrylate/methacrylate allergen content over time in patch test preparations but this has not yet been documented. Objectives To determine the allergen content of acrylates/methacrylates in patch test preparations over time under different storage conditions. Methods Five acrylate/methacrylate allergens [2-hydroxyethyl methacrylate (2-HEMA), methyl methacrylate (MMA), ethylene glycol dimethacrylate (EGDMA), triethylene glycol diacrylate (TREGDA) and 2-hydroxypropyl acrylate (2-HPA)] in syringes and IQ (TM) chambers (Chemotechnique Diagnostics, Vellinge, Sweden) were analysed using gel permeation chromatography and high-performance liquid chromatography to measure the allergen content over time in samples stored in the freezer, refrigerator and under room temperature. Results The concentration of allergens in syringes decreased with time. Those stored at room temperature had the fastest rate of decrease, followed by those in the refrigerator and freezer. In most cases, in syringes or IQ (TM) chambers under all storage conditions, the MMA decreased most rapidly, followed by 2-HPA, 2-HEMA, EGDMA and TREGDA. The allergens in the IQ (TM) chambers rapidly disappeared, with almost all samples reaching nondetectable levels by day 8. MMA was the first to reach a nondetectable level - at day 2. Conclusions Acrylate/methacrylate allergens are lost rapidly from IQ (TM) chambers especially if stored at room temperature. Allergens in syringes remain above 80% of their initial concentrations for longer periods compared with IQ (TM) chambers. In syringes and IQ (TM) chambers there is a slower rate of decrease in concentration when the storage temperature is lower. Allergens should be stored refrigerated, replaced regularly, and freshly applied on to test patches on the day of use.},
  author       = {Goon, Anthony and Bruze, Magnus and Zimerson, Erik and Sörensen, Östen and Goh, C. L. and Koh, D. S. Q. and Isaksson, Marléne},
  issn         = {1365-2133},
  language     = {eng},
  number       = {1},
  pages        = {116--124},
  publisher    = {Wiley-Blackwell},
  series       = {British Journal of Dermatology},
  title        = {Variation in allergen content over time of acrylates/methacrylates in patch test preparations},
  url          = {http://dx.doi.org/10.1111/j.1365-2133.2010.10087.x},
  volume       = {164},
  year         = {2011},
}