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Sperm chromatin structure assay (SCSA): a tool in diagnosis and treatment of infertility

Bungum, Mona LU ; Bungum, Leif LU and Giwercman, Aleksander LU (2011) In Asian Journal of Andrology 13(1). p.69-75
Abstract
Diagnosis of male infertility has mainly been based on the World Health Organization (WHO) manual-based semen parameter's concentration, motility and morphology. It has, however, become apparent that none of these parameters are reliable markers for evaluation of the fertility potential of a couple. A search for better markers has led to an increased focus on sperm chromatin integrity testing in fertility work-up and assisted reproductive techniques. During the last couple of decades, numerous sperm DNA integrity tests have been developed. These are claimed to be characterized by a lower intraindividual variation, less intralaboratory and interlaboratory variation and thus less subjective than the conventional sperm analysis. However, not... (More)
Diagnosis of male infertility has mainly been based on the World Health Organization (WHO) manual-based semen parameter's concentration, motility and morphology. It has, however, become apparent that none of these parameters are reliable markers for evaluation of the fertility potential of a couple. A search for better markers has led to an increased focus on sperm chromatin integrity testing in fertility work-up and assisted reproductive techniques. During the last couple of decades, numerous sperm DNA integrity tests have been developed. These are claimed to be characterized by a lower intraindividual variation, less intralaboratory and interlaboratory variation and thus less subjective than the conventional sperm analysis. However, not all the sperm chromatin integrity tests have yet been shown to be of clinical value. So far, the test that has been found to have the most stable clinical threshold values in relation to fertility is the sperm chromatin structure assay (SCSA), a flow cytometric test that measures the susceptibility of sperm DNA to acid-induced DNA denaturation in situ. Sperm DNA fragmentation as measured by SCSA has shown to be an independent predictor of successful pregnancy in first pregnancy planners as well as in couples undergoing intrauterine insemination, and can be used as a tool in investigation, counseling and treatment of involuntary childlessness. More conflicting data exist regarding the role of sperm DNA fragmentation in relation to fertilization, pre-embryo development and pregnancy outcome in in vitro fertilization and intracytoplasmic sperm injection (ICSI). Asian Journal of Andrology (2011) 13, 69-75; doi: 10.1038/aja.2010.73; published online 8 November 2010 (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
assisted reproductive techniques, infertility, sperm chromatin, structure assay, sperm DNA
in
Asian Journal of Andrology
volume
13
issue
1
pages
69 - 75
publisher
Nature Publishing Group
external identifiers
  • wos:000285869600011
  • scopus:78650996550
ISSN
1008-682X
DOI
10.1038/aja.2010.73
language
English
LU publication?
yes
id
f6c19092-e631-40b6-8384-b1b61d227893 (old id 1791044)
date added to LUP
2011-03-02 14:38:08
date last changed
2017-10-22 03:30:25
@article{f6c19092-e631-40b6-8384-b1b61d227893,
  abstract     = {Diagnosis of male infertility has mainly been based on the World Health Organization (WHO) manual-based semen parameter's concentration, motility and morphology. It has, however, become apparent that none of these parameters are reliable markers for evaluation of the fertility potential of a couple. A search for better markers has led to an increased focus on sperm chromatin integrity testing in fertility work-up and assisted reproductive techniques. During the last couple of decades, numerous sperm DNA integrity tests have been developed. These are claimed to be characterized by a lower intraindividual variation, less intralaboratory and interlaboratory variation and thus less subjective than the conventional sperm analysis. However, not all the sperm chromatin integrity tests have yet been shown to be of clinical value. So far, the test that has been found to have the most stable clinical threshold values in relation to fertility is the sperm chromatin structure assay (SCSA), a flow cytometric test that measures the susceptibility of sperm DNA to acid-induced DNA denaturation in situ. Sperm DNA fragmentation as measured by SCSA has shown to be an independent predictor of successful pregnancy in first pregnancy planners as well as in couples undergoing intrauterine insemination, and can be used as a tool in investigation, counseling and treatment of involuntary childlessness. More conflicting data exist regarding the role of sperm DNA fragmentation in relation to fertilization, pre-embryo development and pregnancy outcome in in vitro fertilization and intracytoplasmic sperm injection (ICSI). Asian Journal of Andrology (2011) 13, 69-75; doi: 10.1038/aja.2010.73; published online 8 November 2010},
  author       = {Bungum, Mona and Bungum, Leif and Giwercman, Aleksander},
  issn         = {1008-682X},
  keyword      = {assisted reproductive techniques,infertility,sperm chromatin,structure assay,sperm DNA},
  language     = {eng},
  number       = {1},
  pages        = {69--75},
  publisher    = {Nature Publishing Group},
  series       = {Asian Journal of Andrology},
  title        = {Sperm chromatin structure assay (SCSA): a tool in diagnosis and treatment of infertility},
  url          = {http://dx.doi.org/10.1038/aja.2010.73},
  volume       = {13},
  year         = {2011},
}