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In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum

Toudahl Nielsen, Jakob ; Kulminskaya, Natalia V. ; Bjerring, Morten ; Linnanto, Juha M. ; Rätsep, Margus ; Östergaard Pedersen, Marie ; Lambrev, Petar H. ; Dorogi, Márta ; Garab, Gyõzõ and Thomsen, Karen , et al. (2016) In Nature Communications 7.
Abstract
Photosynthetic antenna systems enable organisms harvesting light and transfer the energy to the photosynthetic reaction centre, where the conversion to chemical energy takes place. One of the most complex antenna systems, the chlorosome, found in the photosynthetic green sulfur bacterium Chlorobaculum (Cba.) tepidum contains a baseplate, which is a scaffolding super-structure, formed by the protein CsmA and bacteriochlorophyll a. Here we present the first high-resolution structure of the CsmA baseplate using intact fully functional, light-harvesting organelles from Cba. tepidum, following a hybrid approach combining five complementary methods: solid-state NMR spectroscopy, cryo-electron microscopy, isotropic and anisotropic circular... (More)
Photosynthetic antenna systems enable organisms harvesting light and transfer the energy to the photosynthetic reaction centre, where the conversion to chemical energy takes place. One of the most complex antenna systems, the chlorosome, found in the photosynthetic green sulfur bacterium Chlorobaculum (Cba.) tepidum contains a baseplate, which is a scaffolding super-structure, formed by the protein CsmA and bacteriochlorophyll a. Here we present the first high-resolution structure of the CsmA baseplate using intact fully functional, light-harvesting organelles from Cba. tepidum, following a hybrid approach combining five complementary methods: solid-state NMR spectroscopy, cryo-electron microscopy, isotropic and anisotropic circular dichroism and linear dichroism. The structure calculation was facilitated through development of new software, GASyCS for efficient geometry optimization of highly symmetric oligomeric structures. We show that the baseplate is composed of rods of repeated dimers of the strongly amphipathic CsmA with pigments sandwiched within the dimer at the hydrophobic side of the helix. (Less)
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publishing date
type
Contribution to journal
publication status
published
in
Nature Communications
volume
7
article number
12454
pages
12 pages
publisher
Nature Publishing Group
external identifiers
  • scopus:84983283811
ISSN
2041-1723
DOI
10.1038/ncomms12454
language
English
LU publication?
no
id
17adb2e1-13d2-4bb8-b58a-f34bb6627b30
date added to LUP
2020-01-20 15:07:37
date last changed
2024-01-31 16:04:56
@article{17adb2e1-13d2-4bb8-b58a-f34bb6627b30,
  abstract     = {{Photosynthetic antenna systems enable organisms harvesting light and transfer the energy to the photosynthetic reaction centre, where the conversion to chemical energy takes place. One of the most complex antenna systems, the chlorosome, found in the photosynthetic green sulfur bacterium Chlorobaculum (Cba.) tepidum contains a baseplate, which is a scaffolding super-structure, formed by the protein CsmA and bacteriochlorophyll a. Here we present the first high-resolution structure of the CsmA baseplate using intact fully functional, light-harvesting organelles from Cba. tepidum, following a hybrid approach combining five complementary methods: solid-state NMR spectroscopy, cryo-electron microscopy, isotropic and anisotropic circular dichroism and linear dichroism. The structure calculation was facilitated through development of new software, GASyCS for efficient geometry optimization of highly symmetric oligomeric structures. We show that the baseplate is composed of rods of repeated dimers of the strongly amphipathic CsmA with pigments sandwiched within the dimer at the hydrophobic side of the helix.}},
  author       = {{Toudahl Nielsen, Jakob and Kulminskaya, Natalia V. and Bjerring, Morten and Linnanto, Juha M. and Rätsep, Margus and Östergaard Pedersen, Marie and Lambrev, Petar H. and Dorogi, Márta and Garab, Gyõzõ and Thomsen, Karen and Jegerschold, Caroline and Frigaard, Niels-Ulrik and Lindahl, Martin and Nielsen, Niels Chr.}},
  issn         = {{2041-1723}},
  language     = {{eng}},
  month        = {{08}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Nature Communications}},
  title        = {{In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum}},
  url          = {{http://dx.doi.org/10.1038/ncomms12454}},
  doi          = {{10.1038/ncomms12454}},
  volume       = {{7}},
  year         = {{2016}},
}