Soil bacterial growth after a freezing/thawing event
(2016) In Soil Biology and Biochemistry 100. p.229-232- Abstract
Bacterial growth after freezing/thawing was studied in two soils with a history of annual freezing/thawing events. Soil samples were frozen for 1 week at −3 °C or −18 °C, thawed at +4 °C, and respiration and bacterial growth (estimated using leucine incorporation) were compared with reference soils kept at +4 °C. There were no major differences between soils. A respiration pulse, peaking within 9 h, was found, but after 30–100 h respiration had decreased to that in the reference. Freezing at −18 °C resulted in 2.2–2.5 times higher cumulative respiration than the reference, while at −3 °C 1.6–1.8 times higher respiration was found. Bacterial growth rates immediately after thawing were 43–44% of the reference in the −3 °C and 23–26% in... (More)
Bacterial growth after freezing/thawing was studied in two soils with a history of annual freezing/thawing events. Soil samples were frozen for 1 week at −3 °C or −18 °C, thawed at +4 °C, and respiration and bacterial growth (estimated using leucine incorporation) were compared with reference soils kept at +4 °C. There were no major differences between soils. A respiration pulse, peaking within 9 h, was found, but after 30–100 h respiration had decreased to that in the reference. Freezing at −18 °C resulted in 2.2–2.5 times higher cumulative respiration than the reference, while at −3 °C 1.6–1.8 times higher respiration was found. Bacterial growth rates immediately after thawing were 43–44% of the reference in the −3 °C and 23–26% in the −18 °C treatment. Growth rates then increased linearly, recovering after 36 h and around 50 h in the −3 °C and −18 °C freezing, respectively. Growth rates then increased further in the −18 °C, but remained lower or similar to the reference in the −3 °C treatment. The microbial response to freezing/thawing thus appeared similar to mild drying/rewetting (type 1 response sensu Meisner et al. (2015)).
(Less)
- author
- Koponen, Hannu T. and Bååth, Erland LU
- organization
- publishing date
- 2016-09-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bacterial growth, Drying/rewetting, Freezing/thawing, Leucine incorporation, Type 1 response
- in
- Soil Biology and Biochemistry
- volume
- 100
- pages
- 4 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:85008600517
- wos:000380600100026
- ISSN
- 0038-0717
- DOI
- 10.1016/j.soilbio.2016.06.029
- language
- English
- LU publication?
- yes
- id
- 17e4a6dc-6521-405b-92ac-da63f48a31f0
- date added to LUP
- 2017-01-23 13:41:42
- date last changed
- 2024-02-03 09:06:08
@article{17e4a6dc-6521-405b-92ac-da63f48a31f0,
abstract = {{<p>Bacterial growth after freezing/thawing was studied in two soils with a history of annual freezing/thawing events. Soil samples were frozen for 1 week at −3 °C or −18 °C, thawed at +4 °C, and respiration and bacterial growth (estimated using leucine incorporation) were compared with reference soils kept at +4 °C. There were no major differences between soils. A respiration pulse, peaking within 9 h, was found, but after 30–100 h respiration had decreased to that in the reference. Freezing at −18 °C resulted in 2.2–2.5 times higher cumulative respiration than the reference, while at −3 °C 1.6–1.8 times higher respiration was found. Bacterial growth rates immediately after thawing were 43–44% of the reference in the −3 °C and 23–26% in the −18 °C treatment. Growth rates then increased linearly, recovering after 36 h and around 50 h in the −3 °C and −18 °C freezing, respectively. Growth rates then increased further in the −18 °C, but remained lower or similar to the reference in the −3 °C treatment. The microbial response to freezing/thawing thus appeared similar to mild drying/rewetting (type 1 response sensu Meisner et al. (2015)).</p>}},
author = {{Koponen, Hannu T. and Bååth, Erland}},
issn = {{0038-0717}},
keywords = {{Bacterial growth; Drying/rewetting; Freezing/thawing; Leucine incorporation; Type 1 response}},
language = {{eng}},
month = {{09}},
pages = {{229--232}},
publisher = {{Elsevier}},
series = {{Soil Biology and Biochemistry}},
title = {{Soil bacterial growth after a freezing/thawing event}},
url = {{http://dx.doi.org/10.1016/j.soilbio.2016.06.029}},
doi = {{10.1016/j.soilbio.2016.06.029}},
volume = {{100}},
year = {{2016}},
}