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Soil bacterial growth after a freezing/thawing event

Koponen, Hannu T. and Bååth, Erland LU (2016) In Soil Biology and Biochemistry 100. p.229-232
Abstract

Bacterial growth after freezing/thawing was studied in two soils with a history of annual freezing/thawing events. Soil samples were frozen for 1 week at −3 °C or −18 °C, thawed at +4 °C, and respiration and bacterial growth (estimated using leucine incorporation) were compared with reference soils kept at +4 °C. There were no major differences between soils. A respiration pulse, peaking within 9 h, was found, but after 30–100 h respiration had decreased to that in the reference. Freezing at −18 °C resulted in 2.2–2.5 times higher cumulative respiration than the reference, while at −3 °C 1.6–1.8 times higher respiration was found. Bacterial growth rates immediately after thawing were 43–44% of the reference in the −3 °C and 23–26% in... (More)

Bacterial growth after freezing/thawing was studied in two soils with a history of annual freezing/thawing events. Soil samples were frozen for 1 week at −3 °C or −18 °C, thawed at +4 °C, and respiration and bacterial growth (estimated using leucine incorporation) were compared with reference soils kept at +4 °C. There were no major differences between soils. A respiration pulse, peaking within 9 h, was found, but after 30–100 h respiration had decreased to that in the reference. Freezing at −18 °C resulted in 2.2–2.5 times higher cumulative respiration than the reference, while at −3 °C 1.6–1.8 times higher respiration was found. Bacterial growth rates immediately after thawing were 43–44% of the reference in the −3 °C and 23–26% in the −18 °C treatment. Growth rates then increased linearly, recovering after 36 h and around 50 h in the −3 °C and −18 °C freezing, respectively. Growth rates then increased further in the −18 °C, but remained lower or similar to the reference in the −3 °C treatment. The microbial response to freezing/thawing thus appeared similar to mild drying/rewetting (type 1 response sensu Meisner et al. (2015)).

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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Bacterial growth, Drying/rewetting, Freezing/thawing, Leucine incorporation, Type 1 response
in
Soil Biology and Biochemistry
volume
100
pages
4 pages
publisher
Elsevier
external identifiers
  • scopus:85008600517
  • wos:000380600100026
ISSN
0038-0717
DOI
10.1016/j.soilbio.2016.06.029
language
English
LU publication?
yes
id
17e4a6dc-6521-405b-92ac-da63f48a31f0
date added to LUP
2017-01-23 13:41:42
date last changed
2017-09-18 11:33:16
@article{17e4a6dc-6521-405b-92ac-da63f48a31f0,
  abstract     = {<p>Bacterial growth after freezing/thawing was studied in two soils with a history of annual freezing/thawing events. Soil samples were frozen for 1 week at −3 °C or −18 °C, thawed at +4 °C, and respiration and bacterial growth (estimated using leucine incorporation) were compared with reference soils kept at +4 °C. There were no major differences between soils. A respiration pulse, peaking within 9 h, was found, but after 30–100 h respiration had decreased to that in the reference. Freezing at −18 °C resulted in 2.2–2.5 times higher cumulative respiration than the reference, while at −3 °C 1.6–1.8 times higher respiration was found. Bacterial growth rates immediately after thawing were 43–44% of the reference in the −3 °C and 23–26% in the −18 °C treatment. Growth rates then increased linearly, recovering after 36 h and around 50 h in the −3 °C and −18 °C freezing, respectively. Growth rates then increased further in the −18 °C, but remained lower or similar to the reference in the −3 °C treatment. The microbial response to freezing/thawing thus appeared similar to mild drying/rewetting (type 1 response sensu Meisner et al. (2015)).</p>},
  author       = {Koponen, Hannu T. and Bååth, Erland},
  issn         = {0038-0717},
  keyword      = {Bacterial growth,Drying/rewetting,Freezing/thawing,Leucine incorporation,Type 1 response},
  language     = {eng},
  month        = {09},
  pages        = {229--232},
  publisher    = {Elsevier},
  series       = {Soil Biology and Biochemistry},
  title        = {Soil bacterial growth after a freezing/thawing event},
  url          = {http://dx.doi.org/10.1016/j.soilbio.2016.06.029},
  volume       = {100},
  year         = {2016},
}