Advanced

Distinct gene expression profiles in subsets of chronic lymphocytic leukemia expressing stereotyped IGHV4-34 B-cell receptors

Marincevic, Millaray; Mansouri, Mahmoud; Kanduri, Meena; Isaksson, Anders; Goransson, Hanna; Smedby, Karin Ekstrom; Jurlander, Jesper; Juliusson, Gunnar LU ; Davi, Fred and Stamatopoulos, Kostas, et al. (2010) In Haematologica 95(12). p.2072-2079
Abstract
Background Numerous subsets of patients with chronic lymphocytic leukemia display similar immunoglobulin gene usage with almost identical complementarity determining region 3 sequences. Among IGHV4-34 cases, two such subsets with "stereotyped" B-cell receptors were recently identified, i.e. subset #4 (IGHV4-34/IGKV2-30) and subset #16 (IGHV4-34/IGKV3-20). Subset #4 patients appear to share biological and clinical features, e.g. young age at diagnosis and indolent disease, whereas little is known about subset #16 at a clinical level. Design and Methods We investigated the global gene expression pattern in sorted chronic lymphocytic leukemia cells from 25 subset/non-subset IGHV4-34 patients using Affymetrix gene expression arrays. Results... (More)
Background Numerous subsets of patients with chronic lymphocytic leukemia display similar immunoglobulin gene usage with almost identical complementarity determining region 3 sequences. Among IGHV4-34 cases, two such subsets with "stereotyped" B-cell receptors were recently identified, i.e. subset #4 (IGHV4-34/IGKV2-30) and subset #16 (IGHV4-34/IGKV3-20). Subset #4 patients appear to share biological and clinical features, e.g. young age at diagnosis and indolent disease, whereas little is known about subset #16 at a clinical level. Design and Methods We investigated the global gene expression pattern in sorted chronic lymphocytic leukemia cells from 25 subset/non-subset IGHV4-34 patients using Affymetrix gene expression arrays. Results Although generally few differences were found when comparing subset to non-subset 4/16 IGHV4-34 cases, distinct gene expression profiles were revealed for subset #4 versus subset #16. The differentially expressed genes, predominantly with lower expression in subset #4 patients, are involved in important cell regulatory pathways including cell-cycle control, proliferation and immune response, which may partly explain the low-proliferative disease observed in subset #4 patients. Conclusions Our novel data demonstrate distinct gene expression profiles among patients with stereotyped IGHV4-34 B-cell receptors, providing further evidence for biological differences in the pathogenesis of these subsets and underscoring the functional relevance of subset assignment based on B-cell receptor sequence features. (Less)
Please use this url to cite or link to this publication:
author
, et al. (More)
(Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
expression, gene, chronic lymphocytic leukemia, IGHV(4)-34, stereotyped BCR
in
Haematologica
volume
95
issue
12
pages
2072 - 2079
publisher
Ferrata Storti Foundation
external identifiers
  • wos:000285571400013
  • scopus:78649833413
ISSN
1592-8721
DOI
10.3324/haematol.2010.028639
language
English
LU publication?
yes
id
c7e29331-829e-40ed-8882-91bfe3f5ddc9 (old id 1815251)
date added to LUP
2011-03-02 12:43:06
date last changed
2018-05-29 12:27:25
@article{c7e29331-829e-40ed-8882-91bfe3f5ddc9,
  abstract     = {Background Numerous subsets of patients with chronic lymphocytic leukemia display similar immunoglobulin gene usage with almost identical complementarity determining region 3 sequences. Among IGHV4-34 cases, two such subsets with "stereotyped" B-cell receptors were recently identified, i.e. subset #4 (IGHV4-34/IGKV2-30) and subset #16 (IGHV4-34/IGKV3-20). Subset #4 patients appear to share biological and clinical features, e.g. young age at diagnosis and indolent disease, whereas little is known about subset #16 at a clinical level. Design and Methods We investigated the global gene expression pattern in sorted chronic lymphocytic leukemia cells from 25 subset/non-subset IGHV4-34 patients using Affymetrix gene expression arrays. Results Although generally few differences were found when comparing subset to non-subset 4/16 IGHV4-34 cases, distinct gene expression profiles were revealed for subset #4 versus subset #16. The differentially expressed genes, predominantly with lower expression in subset #4 patients, are involved in important cell regulatory pathways including cell-cycle control, proliferation and immune response, which may partly explain the low-proliferative disease observed in subset #4 patients. Conclusions Our novel data demonstrate distinct gene expression profiles among patients with stereotyped IGHV4-34 B-cell receptors, providing further evidence for biological differences in the pathogenesis of these subsets and underscoring the functional relevance of subset assignment based on B-cell receptor sequence features.},
  author       = {Marincevic, Millaray and Mansouri, Mahmoud and Kanduri, Meena and Isaksson, Anders and Goransson, Hanna and Smedby, Karin Ekstrom and Jurlander, Jesper and Juliusson, Gunnar and Davi, Fred and Stamatopoulos, Kostas and Rosenquist, Richard},
  issn         = {1592-8721},
  keyword      = {expression,gene,chronic lymphocytic leukemia,IGHV(4)-34,stereotyped BCR},
  language     = {eng},
  number       = {12},
  pages        = {2072--2079},
  publisher    = {Ferrata Storti Foundation},
  series       = {Haematologica},
  title        = {Distinct gene expression profiles in subsets of chronic lymphocytic leukemia expressing stereotyped IGHV4-34 B-cell receptors},
  url          = {http://dx.doi.org/10.3324/haematol.2010.028639},
  volume       = {95},
  year         = {2010},
}