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Development of a flow injection capillary chemiluminescent ELISA using an imprinted polymer instead of the antibody

Surugiu, Ioana ; Svitel, J. LU ; Ye, L. LU ; Haupt, K. LU and Danielsson, B. LU (2001) In Analytical Chemistry 73(17). p.4388-4392
Abstract

A flow injection competitive assay analogous to enzyme immunoassays has been developed using a molecularly imprinted polymer instead of the antibody. A glass capillary was modified by covalently attaching an imprinted polymer to the inner capillary wall. The herbicide 2,4-dichlorophenoxyacetic acid was used as a model analyte. The analyte was labeled with tobacco peroxidase, and chemiluminescence was used for detection in combination with a photomultiplier tube or a CCD camera. In a competitive mode, the analyte-peroxidase conjugate was passed together with the free analyte through the polymer-coated capillary mounted in a flow system. After a washing step, the chemiluminescent substrate was injected and the bound fraction of the... (More)

A flow injection competitive assay analogous to enzyme immunoassays has been developed using a molecularly imprinted polymer instead of the antibody. A glass capillary was modified by covalently attaching an imprinted polymer to the inner capillary wall. The herbicide 2,4-dichlorophenoxyacetic acid was used as a model analyte. The analyte was labeled with tobacco peroxidase, and chemiluminescence was used for detection in combination with a photomultiplier tube or a CCD camera. In a competitive mode, the analyte-peroxidase conjugate was passed together with the free analyte through the polymer-coated capillary mounted in a flow system. After a washing step, the chemiluminescent substrate was injected and the bound fraction of the conjugate was quantified by measuring the intensity of the emitted light. Calibration curves corresponding to analyte concentrations ranging from 0.5 ng mL-1 to 50 μg mL-1 (2.25 nM-225 μM) were obtained. A lowered detection limit by 2 orders of magnitude was obtained when detection was done in discontinuous mode and the chemiluminescence light was conducted inside the photomultiplier tube by an optical fiber bundle, thus yielding a dynamic range of 5 pg mL-1-100 ng mL-1 (22.5 pM-450 nM).

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
73
issue
17
pages
5 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:0035449475
  • pmid:11569836
ISSN
0003-2700
DOI
10.1021/ac0101757
language
English
LU publication?
yes
id
181e45fb-25f8-41f9-ab4e-9e06e1a1942a
date added to LUP
2019-06-18 09:01:41
date last changed
2021-03-24 05:42:56
@article{181e45fb-25f8-41f9-ab4e-9e06e1a1942a,
  abstract     = {<p>A flow injection competitive assay analogous to enzyme immunoassays has been developed using a molecularly imprinted polymer instead of the antibody. A glass capillary was modified by covalently attaching an imprinted polymer to the inner capillary wall. The herbicide 2,4-dichlorophenoxyacetic acid was used as a model analyte. The analyte was labeled with tobacco peroxidase, and chemiluminescence was used for detection in combination with a photomultiplier tube or a CCD camera. In a competitive mode, the analyte-peroxidase conjugate was passed together with the free analyte through the polymer-coated capillary mounted in a flow system. After a washing step, the chemiluminescent substrate was injected and the bound fraction of the conjugate was quantified by measuring the intensity of the emitted light. Calibration curves corresponding to analyte concentrations ranging from 0.5 ng mL<sup>-1</sup> to 50 μg mL<sup>-1</sup> (2.25 nM-225 μM) were obtained. A lowered detection limit by 2 orders of magnitude was obtained when detection was done in discontinuous mode and the chemiluminescence light was conducted inside the photomultiplier tube by an optical fiber bundle, thus yielding a dynamic range of 5 pg mL<sup>-1</sup>-100 ng mL<sup>-1</sup> (22.5 pM-450 nM).</p>},
  author       = {Surugiu, Ioana and Svitel, J. and Ye, L. and Haupt, K. and Danielsson, B.},
  issn         = {0003-2700},
  language     = {eng},
  month        = {09},
  number       = {17},
  pages        = {4388--4392},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Analytical Chemistry},
  title        = {Development of a flow injection capillary chemiluminescent ELISA using an imprinted polymer instead of the antibody},
  url          = {http://dx.doi.org/10.1021/ac0101757},
  doi          = {10.1021/ac0101757},
  volume       = {73},
  year         = {2001},
}