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Pharmacological and molecular comparison of K-ATP channels in rat basilar and middle cerebral arteries

Ploug, Kenneth Beri; Edvinsson, Lars LU ; Olesen, Jes and Jansen-Olesen, Inger (2006) In European Journal of Pharmacology 553(1-3). p.254-262
Abstract
ATP-sensitive potassium (K-ATP) channels play an important role in the regulation of cerebral vascular tone. In vitro studies using synthetic K-ATP channel openers suggest that the pharmacological profiles differ between rat basilar arteries and rat middle cerebral arteries. To address this issue, we studied the possible involvement of endothelial K-ATP channels by pressurized arteriography after luminal administration of synthetic K-ATP channel openers to rat basilar and middle cerebral arteries. Furthermore, we examined the mRNA and protein expression profile of K-ATP channels to rat basilar and middle cerebral arteries using quantitative real-time PCR (Polymerase Chain Reaction) and Western blotting, respectively. In the per-fusion... (More)
ATP-sensitive potassium (K-ATP) channels play an important role in the regulation of cerebral vascular tone. In vitro studies using synthetic K-ATP channel openers suggest that the pharmacological profiles differ between rat basilar arteries and rat middle cerebral arteries. To address this issue, we studied the possible involvement of endothelial K-ATP channels by pressurized arteriography after luminal administration of synthetic K-ATP channel openers to rat basilar and middle cerebral arteries. Furthermore, we examined the mRNA and protein expression profile of K-ATP channels to rat basilar and middle cerebral arteries using quantitative real-time PCR (Polymerase Chain Reaction) and Western blotting, respectively. In the per-fusion system, we found no significant responses after luminal application of three K-ATP channel openers to rat basilar and middle cerebral arteries. In contrast, abluminal application caused a concentration-dependent dilatation of both arteries, that was more potent in basilar than in middle cerebral arteries. Quantitative real-time PCR detected the presence of mRNA transcripts of the K-ATP channel subunits Kir6.1, Kir6.2, SUR1 and SLTR2B, while SUR2A mRNA was barely detected in both rat basilar and middle cerebral arteries. Of the five mRNAs, the expression levels of Kir6.1 and SUR2B transcripts were predominant in both rat basilar and middle cerebral arteries. Western blotting detected the presence of Kir6.1, Kir6.2, SUR1 and SUR2B proteins in both arteries. Densitometric measurements of the Western blot signals further showed higher expression levels of Kir6.1 and SUR2B proteins in rat middle cerebral arteries than was found in rat basilar arteries. In conclusion, our in vitro pharmacological studies showed no evidence for functional endothelial K-ATP channels in either artery. Furthermore, the results indicate that Kir6.1/SUR2B is the major K-ATP channel complex in rat basilar and middle cerebral arteries. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
quantitative real-time PCR, western blotting, pressurized arteriography, cerebral artery, middle, ATP-sensitive K+ channels (K-ATP channels), basilar artery
in
European Journal of Pharmacology
volume
553
issue
1-3
pages
254 - 262
publisher
Elsevier
external identifiers
  • wos:000242772600035
  • scopus:33751186878
ISSN
1879-0712
DOI
10.1016/j.ejphar.2006.09.053
language
English
LU publication?
yes
id
181fde04-c094-4554-bce9-1ff75c251bb4 (old id 682307)
date added to LUP
2007-12-20 13:54:56
date last changed
2019-10-13 03:24:52
@article{181fde04-c094-4554-bce9-1ff75c251bb4,
  abstract     = {ATP-sensitive potassium (K-ATP) channels play an important role in the regulation of cerebral vascular tone. In vitro studies using synthetic K-ATP channel openers suggest that the pharmacological profiles differ between rat basilar arteries and rat middle cerebral arteries. To address this issue, we studied the possible involvement of endothelial K-ATP channels by pressurized arteriography after luminal administration of synthetic K-ATP channel openers to rat basilar and middle cerebral arteries. Furthermore, we examined the mRNA and protein expression profile of K-ATP channels to rat basilar and middle cerebral arteries using quantitative real-time PCR (Polymerase Chain Reaction) and Western blotting, respectively. In the per-fusion system, we found no significant responses after luminal application of three K-ATP channel openers to rat basilar and middle cerebral arteries. In contrast, abluminal application caused a concentration-dependent dilatation of both arteries, that was more potent in basilar than in middle cerebral arteries. Quantitative real-time PCR detected the presence of mRNA transcripts of the K-ATP channel subunits Kir6.1, Kir6.2, SUR1 and SLTR2B, while SUR2A mRNA was barely detected in both rat basilar and middle cerebral arteries. Of the five mRNAs, the expression levels of Kir6.1 and SUR2B transcripts were predominant in both rat basilar and middle cerebral arteries. Western blotting detected the presence of Kir6.1, Kir6.2, SUR1 and SUR2B proteins in both arteries. Densitometric measurements of the Western blot signals further showed higher expression levels of Kir6.1 and SUR2B proteins in rat middle cerebral arteries than was found in rat basilar arteries. In conclusion, our in vitro pharmacological studies showed no evidence for functional endothelial K-ATP channels in either artery. Furthermore, the results indicate that Kir6.1/SUR2B is the major K-ATP channel complex in rat basilar and middle cerebral arteries.},
  author       = {Ploug, Kenneth Beri and Edvinsson, Lars and Olesen, Jes and Jansen-Olesen, Inger},
  issn         = {1879-0712},
  keyword      = {quantitative real-time PCR,western blotting,pressurized arteriography,cerebral artery,middle,ATP-sensitive K+ channels (K-ATP channels),basilar artery},
  language     = {eng},
  number       = {1-3},
  pages        = {254--262},
  publisher    = {Elsevier},
  series       = {European Journal of Pharmacology},
  title        = {Pharmacological and molecular comparison of K-ATP channels in rat basilar and middle cerebral arteries},
  url          = {http://dx.doi.org/10.1016/j.ejphar.2006.09.053},
  volume       = {553},
  year         = {2006},
}