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Spinal Cord FAAH in Normal Micturition Control and Bladder Overactivity in Awake Rats

Fuellhase, Claudius; Russo, Andrea; Castiglione, Fabio; Benigni, Fabio; Campeau, Lysanne; Montorsi, Francesco; Gratzke, Christian; Bettiga, Arianna; Stief, Christian and Andersson, Karl-Erik, et al. (2013) In Journal of Urology 189(6). p.2364-2370
Abstract
Purpose: We assessed whether spinal inhibition of the cannabinoid degrading enzyme FAAH would have urodynamic effects in normal rats and rats with bladder overactivity induced by partial urethral obstruction or prostaglandin E2. We also determined the expression of FAAH, and the cannabinoid receptors CB1 and CB2 in the sacral spinal cord. Materials and Methods: We used 44 rats for functional (cystometry) and Western blot experiments. The FAAH inhibitor oleoyl ethyl amide (3 to 300 nmol) was administered intrathecally (subarachnoidally) or intravenously. The expression of FAAH and CB1/CB2 receptors was determined by Western blot. Results: Oleoyl ethyl amide given intrathecally affected micturition in normal rats and rats with bladder... (More)
Purpose: We assessed whether spinal inhibition of the cannabinoid degrading enzyme FAAH would have urodynamic effects in normal rats and rats with bladder overactivity induced by partial urethral obstruction or prostaglandin E2. We also determined the expression of FAAH, and the cannabinoid receptors CB1 and CB2 in the sacral spinal cord. Materials and Methods: We used 44 rats for functional (cystometry) and Western blot experiments. The FAAH inhibitor oleoyl ethyl amide (3 to 300 nmol) was administered intrathecally (subarachnoidally) or intravenously. The expression of FAAH and CB1/CB2 receptors was determined by Western blot. Results: Oleoyl ethyl amide given intrathecally affected micturition in normal rats and rats with bladder overactivity but effects were more pronounced in the latter. In normal rats oleoyl ethyl amide only decreased micturition frequency, while it decreased frequency and bladder pressures in rats with bladder overactivity. Intravenous oleoyl ethyl amide (3 to 300 nmol) had no urodynamic effect. FAAH and CB1/CB2 receptors were expressed in the rat sacral spinal cord. The expression of CB1/CB2 receptors but not FAAH was higher in obstructed than in normal rats. Conclusions: FAAH inhibition in the sacral spinal cord by oleoyl ethyl amide resulted in urodynamic effects in normal rats and rats with bladder overactivity. The spinal endocannabinoid system may be involved in normal micturition control and it appears altered when there is bladder overactivity. (Less)
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published
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keywords
fatty-acid amide hydrolase, endocannabinoids, urodynamics, overactive, urinary bladder, urethral obstruction
in
Journal of Urology
volume
189
issue
6
pages
2364 - 2370
publisher
Lippincott Williams & Wilkins
external identifiers
  • wos:000319985900109
  • scopus:84877601035
ISSN
1527-3792
DOI
10.1016/j.juro.2012.11.165
language
English
LU publication?
yes
id
181ff3d0-140f-4562-b76e-fb365d92a955 (old id 3927198)
date added to LUP
2013-08-01 07:35:28
date last changed
2019-08-07 02:23:49
@article{181ff3d0-140f-4562-b76e-fb365d92a955,
  abstract     = {Purpose: We assessed whether spinal inhibition of the cannabinoid degrading enzyme FAAH would have urodynamic effects in normal rats and rats with bladder overactivity induced by partial urethral obstruction or prostaglandin E2. We also determined the expression of FAAH, and the cannabinoid receptors CB1 and CB2 in the sacral spinal cord. Materials and Methods: We used 44 rats for functional (cystometry) and Western blot experiments. The FAAH inhibitor oleoyl ethyl amide (3 to 300 nmol) was administered intrathecally (subarachnoidally) or intravenously. The expression of FAAH and CB1/CB2 receptors was determined by Western blot. Results: Oleoyl ethyl amide given intrathecally affected micturition in normal rats and rats with bladder overactivity but effects were more pronounced in the latter. In normal rats oleoyl ethyl amide only decreased micturition frequency, while it decreased frequency and bladder pressures in rats with bladder overactivity. Intravenous oleoyl ethyl amide (3 to 300 nmol) had no urodynamic effect. FAAH and CB1/CB2 receptors were expressed in the rat sacral spinal cord. The expression of CB1/CB2 receptors but not FAAH was higher in obstructed than in normal rats. Conclusions: FAAH inhibition in the sacral spinal cord by oleoyl ethyl amide resulted in urodynamic effects in normal rats and rats with bladder overactivity. The spinal endocannabinoid system may be involved in normal micturition control and it appears altered when there is bladder overactivity.},
  author       = {Fuellhase, Claudius and Russo, Andrea and Castiglione, Fabio and Benigni, Fabio and Campeau, Lysanne and Montorsi, Francesco and Gratzke, Christian and Bettiga, Arianna and Stief, Christian and Andersson, Karl-Erik and Hedlund, Petter},
  issn         = {1527-3792},
  keyword      = {fatty-acid amide hydrolase,endocannabinoids,urodynamics,overactive,urinary bladder,urethral obstruction},
  language     = {eng},
  number       = {6},
  pages        = {2364--2370},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Journal of Urology},
  title        = {Spinal Cord FAAH in Normal Micturition Control and Bladder Overactivity in Awake Rats},
  url          = {http://dx.doi.org/10.1016/j.juro.2012.11.165},
  volume       = {189},
  year         = {2013},
}