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100% complete assignment of non-labile H-1, C-13, and N-15 signals for calcium-loaded calbindin D-9k P43G

Oktaviani, Nur Alia; Otten, Renee; Dijkstra, Klaas; Scheek, Ruud M.; Thulin, Eva LU ; Akke, Mikael LU and Mulder, Frans LU (2011) In Biomolecular NMR Assignments 5(1). p.79-84
Abstract
Here we present the 100% complete assignment chemical shift of non-labile H-1, N-15 and C-13 nuclei of Calbindin D-9k P43G. The assignment includes all non-exchangeable side chain nuclei, including ones that are rarely reported, such as LysN zeta as well as the termini. NMR experiments required to achieve truly complete assignments are discussed. To the best of our knowledge our assignments for Calbindin D-9k extend beyond previous studies reaching near-completeness (Vis et al. in Biochem 33:14858-14870, 1994; Yamazaki et al. in J Am Chem Soc 116:6464-6465, 1994; Yamazaki et al. in Biochem 32:5656-5669, 1993b).
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Calbindin D-9k, 100% Complete assignment, Assignment strategy, NMR, spectroscopy
in
Biomolecular NMR Assignments
volume
5
issue
1
pages
79 - 84
publisher
Springer
external identifiers
  • wos:000288076300020
  • scopus:79952441586
ISSN
1874-2718
DOI
10.1007/s12104-010-9272-3
language
English
LU publication?
yes
id
a9c9f1b6-26a6-438e-ad9f-95de80677069 (old id 1868663)
date added to LUP
2011-04-19 12:02:38
date last changed
2017-01-01 03:42:42
@article{a9c9f1b6-26a6-438e-ad9f-95de80677069,
  abstract     = {Here we present the 100% complete assignment chemical shift of non-labile H-1, N-15 and C-13 nuclei of Calbindin D-9k P43G. The assignment includes all non-exchangeable side chain nuclei, including ones that are rarely reported, such as LysN zeta as well as the termini. NMR experiments required to achieve truly complete assignments are discussed. To the best of our knowledge our assignments for Calbindin D-9k extend beyond previous studies reaching near-completeness (Vis et al. in Biochem 33:14858-14870, 1994; Yamazaki et al. in J Am Chem Soc 116:6464-6465, 1994; Yamazaki et al. in Biochem 32:5656-5669, 1993b).},
  author       = {Oktaviani, Nur Alia and Otten, Renee and Dijkstra, Klaas and Scheek, Ruud M. and Thulin, Eva and Akke, Mikael and Mulder, Frans},
  issn         = {1874-2718},
  keyword      = {Calbindin D-9k,100% Complete assignment,Assignment strategy,NMR,spectroscopy},
  language     = {eng},
  number       = {1},
  pages        = {79--84},
  publisher    = {Springer},
  series       = {Biomolecular NMR Assignments},
  title        = {100% complete assignment of non-labile H-1, C-13, and N-15 signals for calcium-loaded calbindin D-9k P43G},
  url          = {http://dx.doi.org/10.1007/s12104-010-9272-3},
  volume       = {5},
  year         = {2011},
}