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DNA gel particles from single and double-tail surfactants: supramolecular assemblies and release characteristics

Carmen Moran, M.; Graca Miguel, M. and Lindman, Björn LU (2011) In Soft Matter 7(5). p.2001-2010
Abstract
Cationic surfactants with one alkyl chain have offered a particularly efficient control of the properties of DNA-based particles. However, most transfection lipids do not form micelles as single-chain surfactants. In this work, the formation of DNA gel particles mixing DNA (either single- (ssDNA) or double-stranded (dsDNA)) with the vesicle-forming double chain surfactant didodecyldimethylammonium bromide (DDAB) is described. For comparative purposes, the formation of DNA gel particles using the single chain surfactant dodecyltrimethylammonium bromide (DTAB) has been investigated. Results on the encapsulation of DNA and its release are strongly dependent on the surfactant structure. DDAB-DNA gel particles have been shown to form ordered... (More)
Cationic surfactants with one alkyl chain have offered a particularly efficient control of the properties of DNA-based particles. However, most transfection lipids do not form micelles as single-chain surfactants. In this work, the formation of DNA gel particles mixing DNA (either single- (ssDNA) or double-stranded (dsDNA)) with the vesicle-forming double chain surfactant didodecyldimethylammonium bromide (DDAB) is described. For comparative purposes, the formation of DNA gel particles using the single chain surfactant dodecyltrimethylammonium bromide (DTAB) has been investigated. Results on the encapsulation of DNA and its release are strongly dependent on the surfactant structure. DDAB-DNA gel particles have been shown to form ordered multi-vesicular assemblies which resemble the architecture of biological tissues. The characteristic sizes and shapes of the resulting structures strongly depend on the secondary structure of the DNA. Some features about the DNA-vesicle interaction involved in these particles have been deduced from direct optical, fluorescence and electron microscopy observations. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Soft Matter
volume
7
issue
5
pages
2001 - 2010
publisher
Royal Society of Chemistry
external identifiers
  • wos:000287588800058
  • scopus:79951889124
ISSN
1744-6848
DOI
10.1039/c0sm00988a
language
English
LU publication?
yes
id
9e6686df-9f18-4963-9e2a-bd88f81622fa (old id 1869992)
date added to LUP
2011-04-06 11:01:23
date last changed
2017-08-27 04:30:54
@article{9e6686df-9f18-4963-9e2a-bd88f81622fa,
  abstract     = {Cationic surfactants with one alkyl chain have offered a particularly efficient control of the properties of DNA-based particles. However, most transfection lipids do not form micelles as single-chain surfactants. In this work, the formation of DNA gel particles mixing DNA (either single- (ssDNA) or double-stranded (dsDNA)) with the vesicle-forming double chain surfactant didodecyldimethylammonium bromide (DDAB) is described. For comparative purposes, the formation of DNA gel particles using the single chain surfactant dodecyltrimethylammonium bromide (DTAB) has been investigated. Results on the encapsulation of DNA and its release are strongly dependent on the surfactant structure. DDAB-DNA gel particles have been shown to form ordered multi-vesicular assemblies which resemble the architecture of biological tissues. The characteristic sizes and shapes of the resulting structures strongly depend on the secondary structure of the DNA. Some features about the DNA-vesicle interaction involved in these particles have been deduced from direct optical, fluorescence and electron microscopy observations.},
  author       = {Carmen Moran, M. and Graca Miguel, M. and Lindman, Björn},
  issn         = {1744-6848},
  language     = {eng},
  number       = {5},
  pages        = {2001--2010},
  publisher    = {Royal Society of Chemistry},
  series       = {Soft Matter},
  title        = {DNA gel particles from single and double-tail surfactants: supramolecular assemblies and release characteristics},
  url          = {http://dx.doi.org/10.1039/c0sm00988a},
  volume       = {7},
  year         = {2011},
}