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Corticotropin releasing factor-Distribution in rat intestine and role in neuroprotection

Sand, Elin LU ; Themner-Persson, Anna LU and Ekblad, Eva LU (2011) In Regulatory Peptides 166(1-3). p.68-75
Abstract
Aims of the present stud were to describe the distribution of corticotropin releasing factor (CRF) immunoreactivity in rat small and large intestines, to quantify the percentage of CRF-immunoreactive (CRF-IR) enteric neurons, to reveal possible CRF immunoreactivity in cultured myenteric neurons from rat ileum and to examine if additions of CRF, urocortin 1 (Ucn1), CRF antagonist or vasoactive intestinal peptide (VIP) affect neuronal survival in vitro. Co-localization of CRF- and VIP-immunoreactivity was examined, as well as a possible interplay between CRF and VIP in neuroprotection. Further we wanted to elucidate if mast cells affect neuronal survival via CRF signaling. Networks of CRF-containing nerve cell bodies and fibers were detected... (More)
Aims of the present stud were to describe the distribution of corticotropin releasing factor (CRF) immunoreactivity in rat small and large intestines, to quantify the percentage of CRF-immunoreactive (CRF-IR) enteric neurons, to reveal possible CRF immunoreactivity in cultured myenteric neurons from rat ileum and to examine if additions of CRF, urocortin 1 (Ucn1), CRF antagonist or vasoactive intestinal peptide (VIP) affect neuronal survival in vitro. Co-localization of CRF- and VIP-immunoreactivity was examined, as well as a possible interplay between CRF and VIP in neuroprotection. Further we wanted to elucidate if mast cells affect neuronal survival via CRF signaling. Networks of CRF-containing nerve cell bodies and fibers were detected in rat intestine. CRF-IR neurons contained to a high degree also VIP. A low number of cultured myenteric neurons was CRF-IR. CRF, Ucn1 or CRF-antagonist did not promote neuronal survival of cultured myenteric neurons, while VIP significantly enhanced neuronal survival. Simultaneous presence of CRF attenuated the VIP mediated increase in neuronal survival. Co-culturing neurons and mast cells resulted in a marked reduction in neuronal survival, not executed via CRF signaling pathways. Conclusion: CRF is present in enteric neurons and counteracts the neuroprotective effect of VIP in vitro. (c) 2010 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CRF, Mast cells, Myenteric neurons, Primary culture, VIP
in
Regulatory Peptides
volume
166
issue
1-3
pages
68 - 75
publisher
Elsevier
external identifiers
  • wos:000286685200012
  • scopus:78650177287
ISSN
1873-1686
DOI
10.1016/j.regpep.2010.08.011
language
English
LU publication?
yes
id
4e77adb5-2837-4e1e-a5e4-967911d7687b (old id 1876925)
date added to LUP
2011-04-01 10:53:46
date last changed
2017-01-01 03:29:16
@article{4e77adb5-2837-4e1e-a5e4-967911d7687b,
  abstract     = {Aims of the present stud were to describe the distribution of corticotropin releasing factor (CRF) immunoreactivity in rat small and large intestines, to quantify the percentage of CRF-immunoreactive (CRF-IR) enteric neurons, to reveal possible CRF immunoreactivity in cultured myenteric neurons from rat ileum and to examine if additions of CRF, urocortin 1 (Ucn1), CRF antagonist or vasoactive intestinal peptide (VIP) affect neuronal survival in vitro. Co-localization of CRF- and VIP-immunoreactivity was examined, as well as a possible interplay between CRF and VIP in neuroprotection. Further we wanted to elucidate if mast cells affect neuronal survival via CRF signaling. Networks of CRF-containing nerve cell bodies and fibers were detected in rat intestine. CRF-IR neurons contained to a high degree also VIP. A low number of cultured myenteric neurons was CRF-IR. CRF, Ucn1 or CRF-antagonist did not promote neuronal survival of cultured myenteric neurons, while VIP significantly enhanced neuronal survival. Simultaneous presence of CRF attenuated the VIP mediated increase in neuronal survival. Co-culturing neurons and mast cells resulted in a marked reduction in neuronal survival, not executed via CRF signaling pathways. Conclusion: CRF is present in enteric neurons and counteracts the neuroprotective effect of VIP in vitro. (c) 2010 Elsevier B.V. All rights reserved.},
  author       = {Sand, Elin and Themner-Persson, Anna and Ekblad, Eva},
  issn         = {1873-1686},
  keyword      = {CRF,Mast cells,Myenteric neurons,Primary culture,VIP},
  language     = {eng},
  number       = {1-3},
  pages        = {68--75},
  publisher    = {Elsevier},
  series       = {Regulatory Peptides},
  title        = {Corticotropin releasing factor-Distribution in rat intestine and role in neuroprotection},
  url          = {http://dx.doi.org/10.1016/j.regpep.2010.08.011},
  volume       = {166},
  year         = {2011},
}