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In vitro and in vivo evaluation of chemically modified degradable starch microspheres for topical haemostasis.

Björses, Katarina LU ; Faxälv, Lars; Montan, Carl LU ; Persson, Katarina; Fyhr, Peter; Holst, Jan LU and Lindahl, Tomas L (2011) In Acta Biomaterialia 7. p.2558-2565
Abstract
Degradable starch microspheres (DSMs) are starch chains cross-linked with epichlorhydrin, forming glycerol-ether links. DSMs have been used for many years for temporary vascular occlusion and drug delivery in treatment of malignancies. They are also approved and used for topical haemostasis by absorbing excess fluid from the blood and concentrating endogenous coagulation factors, thereby facilitating haemostasis. This mechanism of action is not sufficient for larger bleedings in current chemical formulations of DSMs, and modification of DSMs to trigger activation of platelets or coagulation would be required for use in such applications. Chemical modifications of DSMs with N-octenyl succinic anhydride, chloroacetic acid, acetic anhydride,... (More)
Degradable starch microspheres (DSMs) are starch chains cross-linked with epichlorhydrin, forming glycerol-ether links. DSMs have been used for many years for temporary vascular occlusion and drug delivery in treatment of malignancies. They are also approved and used for topical haemostasis by absorbing excess fluid from the blood and concentrating endogenous coagulation factors, thereby facilitating haemostasis. This mechanism of action is not sufficient for larger bleedings in current chemical formulations of DSMs, and modification of DSMs to trigger activation of platelets or coagulation would be required for use in such applications. Chemical modifications of DSMs with N-octenyl succinic anhydride, chloroacetic acid, acetic anhydride, diethylaminoethyl chloride and ellagic acid were performed and evaluated in vitro with thrombin generation and platelet adhesion tests, and in vivo using an experimental renal bleeding model in rat. DSMs modified to activate platelets in vitro were superior in haemostatic capacity in vivo. Further studies with non-toxic substances are warranted to confirm these results and develop the DSM as a more effective topical haemostatic agent. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Biomaterialia
volume
7
pages
2558 - 2565
publisher
Elsevier
external identifiers
  • wos:000291181800022
  • pmid:21382526
  • scopus:79955636561
ISSN
1878-7568
DOI
10.1016/j.actbio.2011.03.003
language
English
LU publication?
yes
id
36b4f0ea-a744-4dfe-aafd-8eaff92e7c92 (old id 1884266)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21382526?dopt=Abstract
date added to LUP
2011-04-01 13:54:22
date last changed
2017-01-01 07:34:39
@article{36b4f0ea-a744-4dfe-aafd-8eaff92e7c92,
  abstract     = {Degradable starch microspheres (DSMs) are starch chains cross-linked with epichlorhydrin, forming glycerol-ether links. DSMs have been used for many years for temporary vascular occlusion and drug delivery in treatment of malignancies. They are also approved and used for topical haemostasis by absorbing excess fluid from the blood and concentrating endogenous coagulation factors, thereby facilitating haemostasis. This mechanism of action is not sufficient for larger bleedings in current chemical formulations of DSMs, and modification of DSMs to trigger activation of platelets or coagulation would be required for use in such applications. Chemical modifications of DSMs with N-octenyl succinic anhydride, chloroacetic acid, acetic anhydride, diethylaminoethyl chloride and ellagic acid were performed and evaluated in vitro with thrombin generation and platelet adhesion tests, and in vivo using an experimental renal bleeding model in rat. DSMs modified to activate platelets in vitro were superior in haemostatic capacity in vivo. Further studies with non-toxic substances are warranted to confirm these results and develop the DSM as a more effective topical haemostatic agent.},
  author       = {Björses, Katarina and Faxälv, Lars and Montan, Carl and Persson, Katarina and Fyhr, Peter and Holst, Jan and Lindahl, Tomas L},
  issn         = {1878-7568},
  language     = {eng},
  pages        = {2558--2565},
  publisher    = {Elsevier},
  series       = {Acta Biomaterialia},
  title        = {In vitro and in vivo evaluation of chemically modified degradable starch microspheres for topical haemostasis.},
  url          = {http://dx.doi.org/10.1016/j.actbio.2011.03.003},
  volume       = {7},
  year         = {2011},
}