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Differential regulation of alpha and beta chains of C4b-binding protein during acute-phase response resulting in stable plasma levels of free anticoagulant protein S

Garcia de Frutos, Pablo LU ; Alim, R; Härdig, Ylva; Zöller, Bengt LU and Dahlbäck, Björn LU (1994) In Blood 84(3). p.815-822
Abstract

Regulation of C4b-binding protein (C4BP) isoforms during acute phase and its relationship to the plasma concentration of free protein S was elucidated. An assay for beta chain containing C4BP (C4BP beta+) was developed and the concentrations of total C4BP, C4BP beta+, total, free, and bound protein S were measured in patients with acute-phase response. Even though total C4BP was increased to 162% (mean value) of controls, the corresponding value of C4BP beta+ was only 122%. In the acute-phase group, total protein S was increased to the same extent as C4BP beta+ (mean value of 124%), whereas free protein S was not decreased. In controls, total and bound protein S correlated with total C4BP and C4BP beta+. However, in the acute-phase... (More)

Regulation of C4b-binding protein (C4BP) isoforms during acute phase and its relationship to the plasma concentration of free protein S was elucidated. An assay for beta chain containing C4BP (C4BP beta+) was developed and the concentrations of total C4BP, C4BP beta+, total, free, and bound protein S were measured in patients with acute-phase response. Even though total C4BP was increased to 162% (mean value) of controls, the corresponding value of C4BP beta+ was only 122%. In the acute-phase group, total protein S was increased to the same extent as C4BP beta+ (mean value of 124%), whereas free protein S was not decreased. In controls, total and bound protein S correlated with total C4BP and C4BP beta+. However, in the acute-phase group, the correlation between bound protein S and total C4BP was lost, although the correlation between C4BP beta+ and protein S remained. The present results suggest stable levels of free protein S during acute phase to be the result of differential regulation of C4BP alpha- and beta-chain expression, and the concentration of free protein S to be the resulting molar excess of protein S over C4BP beta+. This mechanism ensures functional levels of free anticoagulant protein S despite high levels of C4BP.

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organization
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Contribution to journal
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published
subject
keywords
Acute-Phase Proteins, Acute-Phase Reaction, Antibodies, Monoclonal, Calcium, Carrier Proteins, Complement C4b, Complement Inactivator Proteins, Glycoproteins, Humans, Macromolecular Substances, Protein S, Journal Article, Research Support, Non-U.S. Gov't
in
Blood
volume
84
issue
3
pages
8 pages
publisher
American Society of Hematology
external identifiers
  • scopus:0028103281
ISSN
1528-0020
language
English
LU publication?
yes
id
8bad4052-e453-4a54-870c-4dccca3199f4 (old id 1894793)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/7519078
http://bloodjournal.hematologylibrary.org/content/84/3/815.long
date added to LUP
2013-05-14 15:57:24
date last changed
2018-11-21 21:15:03
@article{8bad4052-e453-4a54-870c-4dccca3199f4,
  abstract     = {<p>Regulation of C4b-binding protein (C4BP) isoforms during acute phase and its relationship to the plasma concentration of free protein S was elucidated. An assay for beta chain containing C4BP (C4BP beta+) was developed and the concentrations of total C4BP, C4BP beta+, total, free, and bound protein S were measured in patients with acute-phase response. Even though total C4BP was increased to 162% (mean value) of controls, the corresponding value of C4BP beta+ was only 122%. In the acute-phase group, total protein S was increased to the same extent as C4BP beta+ (mean value of 124%), whereas free protein S was not decreased. In controls, total and bound protein S correlated with total C4BP and C4BP beta+. However, in the acute-phase group, the correlation between bound protein S and total C4BP was lost, although the correlation between C4BP beta+ and protein S remained. The present results suggest stable levels of free protein S during acute phase to be the result of differential regulation of C4BP alpha- and beta-chain expression, and the concentration of free protein S to be the resulting molar excess of protein S over C4BP beta+. This mechanism ensures functional levels of free anticoagulant protein S despite high levels of C4BP.</p>},
  author       = {Garcia de Frutos, Pablo and Alim, R and Härdig, Ylva and Zöller, Bengt and Dahlbäck, Björn},
  issn         = {1528-0020},
  keyword      = {Acute-Phase Proteins,Acute-Phase Reaction,Antibodies, Monoclonal,Calcium,Carrier Proteins,Complement C4b,Complement Inactivator Proteins,Glycoproteins,Humans,Macromolecular Substances,Protein S,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {3},
  pages        = {815--822},
  publisher    = {American Society of Hematology},
  series       = {Blood},
  title        = {Differential regulation of alpha and beta chains of C4b-binding protein during acute-phase response resulting in stable plasma levels of free anticoagulant protein S},
  volume       = {84},
  year         = {1994},
}