Cannula Implantation into the Cisterna Magna of Rodents
(2018) In Journal of visualized experiments : JoVE- Abstract
Cisterna magna cannulation (CMc) is a straightforward procedure that enables direct access to the cerebrospinal fluid (CSF) without operative damage to the skull or the brain parenchyma. In anesthetized rodents, the exposure of the dura mater by blunt dissection of the neck muscles allows the insertion of a cannula into the cisterna magna (CM). The cannula, composed either by a fine beveled needle or borosilicate capillary, is attached via a polyethylene (PE) tube to a syringe. Using a syringe pump, molecules can then be injected at controlled rates directly into the CM, which is continuous with the subarachnoid space. From the subarachnoid space, we can trace CSF fluxes by convective flow into the perivascular space around penetrating... (More)
Cisterna magna cannulation (CMc) is a straightforward procedure that enables direct access to the cerebrospinal fluid (CSF) without operative damage to the skull or the brain parenchyma. In anesthetized rodents, the exposure of the dura mater by blunt dissection of the neck muscles allows the insertion of a cannula into the cisterna magna (CM). The cannula, composed either by a fine beveled needle or borosilicate capillary, is attached via a polyethylene (PE) tube to a syringe. Using a syringe pump, molecules can then be injected at controlled rates directly into the CM, which is continuous with the subarachnoid space. From the subarachnoid space, we can trace CSF fluxes by convective flow into the perivascular space around penetrating arterioles, where solute exchange with the interstitial fluid (ISF) occurs. CMc can be performed for acute injections immediately following the surgery, or for chronic implantation, with later injection in anesthetized or awake, freely moving rodents. Quantitation of tracer distribution in the brain parenchyma can be performed by epifluorescence, 2-photon microscopy, and magnetic resonance imaging (MRI), depending on the physico-chemical properties of the injected molecules. Thus, CMc in conjunction with various imaging techniques offers a powerful tool for assessment of the glymphatic system and CSF dynamics and function. Furthermore, CMc can be utilized as a conduit for fast, brain-wide delivery of signaling molecules and metabolic substrates that could not otherwise cross the blood brain barrier (BBB).
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- author
- Xavier, Anna L.R. ; Hauglund, Natalie Linea ; von Holstein-Rathlou, Stephanie ; Li, Qianliang ; Sanggaard, Simon ; Lou, Nanhong ; Lundgaard, Iben LU and Nedergaard, Maiken
- organization
- publishing date
- 2018-05-23
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of visualized experiments : JoVE
- issue
- 135
- article number
- e57378
- publisher
- JoVE
- external identifiers
-
- scopus:85051988680
- pmid:29889209
- ISSN
- 1940-087X
- DOI
- 10.3791/57378
- language
- English
- LU publication?
- yes
- id
- 190bb79c-9b87-458c-bfb5-f81a09813428
- date added to LUP
- 2018-09-27 12:30:25
- date last changed
- 2024-04-15 12:05:35
@article{190bb79c-9b87-458c-bfb5-f81a09813428,
abstract = {{<p>Cisterna magna cannulation (CMc) is a straightforward procedure that enables direct access to the cerebrospinal fluid (CSF) without operative damage to the skull or the brain parenchyma. In anesthetized rodents, the exposure of the dura mater by blunt dissection of the neck muscles allows the insertion of a cannula into the cisterna magna (CM). The cannula, composed either by a fine beveled needle or borosilicate capillary, is attached via a polyethylene (PE) tube to a syringe. Using a syringe pump, molecules can then be injected at controlled rates directly into the CM, which is continuous with the subarachnoid space. From the subarachnoid space, we can trace CSF fluxes by convective flow into the perivascular space around penetrating arterioles, where solute exchange with the interstitial fluid (ISF) occurs. CMc can be performed for acute injections immediately following the surgery, or for chronic implantation, with later injection in anesthetized or awake, freely moving rodents. Quantitation of tracer distribution in the brain parenchyma can be performed by epifluorescence, 2-photon microscopy, and magnetic resonance imaging (MRI), depending on the physico-chemical properties of the injected molecules. Thus, CMc in conjunction with various imaging techniques offers a powerful tool for assessment of the glymphatic system and CSF dynamics and function. Furthermore, CMc can be utilized as a conduit for fast, brain-wide delivery of signaling molecules and metabolic substrates that could not otherwise cross the blood brain barrier (BBB).</p>}},
author = {{Xavier, Anna L.R. and Hauglund, Natalie Linea and von Holstein-Rathlou, Stephanie and Li, Qianliang and Sanggaard, Simon and Lou, Nanhong and Lundgaard, Iben and Nedergaard, Maiken}},
issn = {{1940-087X}},
language = {{eng}},
month = {{05}},
number = {{135}},
publisher = {{JoVE}},
series = {{Journal of visualized experiments : JoVE}},
title = {{Cannula Implantation into the Cisterna Magna of Rodents}},
url = {{http://dx.doi.org/10.3791/57378}},
doi = {{10.3791/57378}},
year = {{2018}},
}