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Diffusion studies in gels using holographic laser interferometry

Mattisson, Charlotte LU (1999) In Department of Chemical Engineering 1, Lund, Sweden 1999:1003.
Abstract
This thesis is an investigation of the potential of holographic laser interferometry for the study of diffusion of both small solutes and macromolecules. An investigation on the importance of errors due to light deflection in refarctive index gradients showed that the magnitude of the refractive index gradients were negligible for the system investigated. The holographic interferometry technique requires a high stability to study diffusion of macromolecules. This was achieved by a proper design and hence the diffusion coefficients for BSA, lysozyme and pullulan in agarose gels were succesfully obtained. The effect of solvent as well as the BSA and agarose concentration on the BSA diffusion coefficient and partition coefficient was... (More)
This thesis is an investigation of the potential of holographic laser interferometry for the study of diffusion of both small solutes and macromolecules. An investigation on the importance of errors due to light deflection in refarctive index gradients showed that the magnitude of the refractive index gradients were negligible for the system investigated. The holographic interferometry technique requires a high stability to study diffusion of macromolecules. This was achieved by a proper design and hence the diffusion coefficients for BSA, lysozyme and pullulan in agarose gels were succesfully obtained. The effect of solvent as well as the BSA and agarose concentration on the BSA diffusion coefficient and partition coefficient was investigated. In addition, the effect of the pullulan molar weight on the pullulan diffusion and partition coefficient in agarose gel was studied. The effect of pH and ionic strength on the lysozyme diffusion in agarose gel was studied. The experimental data was evaluated using a general transport model based on chemical potential gradients instead of concentration gradients. The chemical potential was estimated by using the Poisson-Boltzmann equation. (Less)
Please use this url to cite or link to this publication:
author
supervisor
opponent
  • Professor Cussler, E.L., University of Minnesota, Minneapolis, USA
organization
publishing date
type
Thesis
publication status
published
subject
keywords
chemical potential, ionic strenght, pH, cubic phase, partition coefficient, holographic laser interferometry, holography, pullulan, lysozyme, BSA, macromolecules, diffusion, gel, Poisson-Boltzmann, Chemical technology and engineering, Kemiteknik och kemisk teknologi
in
Department of Chemical Engineering 1, Lund, Sweden
volume
1999:1003
pages
176 pages
publisher
Chemical Engineering 1, Lund University
defense location
Lund University, Chemical Centre, lecture hall K:C
defense date
1999-06-11 10:15
external identifiers
  • other:ISRN: LUTHKDH/TKKA-1003/1-169/(1999)
ISSN
1100-2778
language
English
LU publication?
yes
id
1d1d5e0d-d3ca-461d-afb3-f47bc6236ead (old id 19224)
date added to LUP
2007-05-24 14:09:16
date last changed
2016-09-19 08:44:57
@phdthesis{1d1d5e0d-d3ca-461d-afb3-f47bc6236ead,
  abstract     = {This thesis is an investigation of the potential of holographic laser interferometry for the study of diffusion of both small solutes and macromolecules. An investigation on the importance of errors due to light deflection in refarctive index gradients showed that the magnitude of the refractive index gradients were negligible for the system investigated. The holographic interferometry technique requires a high stability to study diffusion of macromolecules. This was achieved by a proper design and hence the diffusion coefficients for BSA, lysozyme and pullulan in agarose gels were succesfully obtained. The effect of solvent as well as the BSA and agarose concentration on the BSA diffusion coefficient and partition coefficient was investigated. In addition, the effect of the pullulan molar weight on the pullulan diffusion and partition coefficient in agarose gel was studied. The effect of pH and ionic strength on the lysozyme diffusion in agarose gel was studied. The experimental data was evaluated using a general transport model based on chemical potential gradients instead of concentration gradients. The chemical potential was estimated by using the Poisson-Boltzmann equation.},
  author       = {Mattisson, Charlotte},
  issn         = {1100-2778},
  keyword      = {chemical potential,ionic strenght,pH,cubic phase,partition coefficient,holographic laser interferometry,holography,pullulan,lysozyme,BSA,macromolecules,diffusion,gel,Poisson-Boltzmann,Chemical technology and engineering,Kemiteknik och kemisk teknologi},
  language     = {eng},
  pages        = {176},
  publisher    = {Chemical Engineering 1, Lund University},
  school       = {Lund University},
  series       = {Department of Chemical Engineering 1, Lund, Sweden},
  title        = {Diffusion studies in gels using holographic laser interferometry},
  volume       = {1999:1003},
  year         = {1999},
}