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Altered mRNA Expression due to Acute Mesenteric Ischaemia in a Porcine Model

Block, T.; Isaksson, H. S.; Acosta, Stefan LU ; Bjorck, M.; Brodin, D. and Nilsson, T. K. (2011) In European Journal of Vascular and Endovascular Surgery 41(2). p.281-287
Abstract
Introduction: Messenger RNA (mRNA) changes in the small intestine in response to acute mesenteric ischaemia (AMI) could offer novel diagnostic possibilities, but have not been described. The aim was to characterize the mRNA response to experimental AMI. Materials and methods: Twelve pigs underwent catheterisation of the superior mesenteric artery with injection of polivinylalcohol embolisation particles or sodium chloride. Laparotomy and intestinal tissue sampling were performed. Microarray analysis was performed using the GeneChip (R) whole porcine genome array. Results: Seven down-regulated cellular pathways were associated with protein, lipid and carbohydrate metabolism. Seventeen up-regulated pathways were associated with inflammatory... (More)
Introduction: Messenger RNA (mRNA) changes in the small intestine in response to acute mesenteric ischaemia (AMI) could offer novel diagnostic possibilities, but have not been described. The aim was to characterize the mRNA response to experimental AMI. Materials and methods: Twelve pigs underwent catheterisation of the superior mesenteric artery with injection of polivinylalcohol embolisation particles or sodium chloride. Laparotomy and intestinal tissue sampling were performed. Microarray analysis was performed using the GeneChip (R) whole porcine genome array. Results: Seven down-regulated cellular pathways were associated with protein, lipid and carbohydrate metabolism. Seventeen up-regulated pathways were associated with inflammatory and immunological activity, regulation of extracellular matrix and decreased cellular proliferation. Thrombospondin (THS), monocyte chemoattractant protein 1(MCP-1) and gap junction alpha 1(GJA-1) were consistently up-regulated in all embolised pigs. Genes encoding earlier proposed biomarkers for AMI were up-regulated, such as lactate dehydrogenase and creatine kinase, or down-regulated, such as intestinal fatty acid binding protein and glutathione S-transferase. Conclusion: This study describes the intestinal tissue response on a gene expression level to AMI. THS, MCP-1 and GJA-1 were consistently up-regulated by ischaemia, whereas earlier proposed biomarkers for AMI were not. Gene expression may not be directly linked to the use of the corresponding proteins as potential clinical biomarkers. (C) 2010 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Superior mesenteric artery, Acute mesenteric ischaemia, Gene expression, analysis, Porcine, Microarray
in
European Journal of Vascular and Endovascular Surgery
volume
41
issue
2
pages
281 - 287
publisher
Elsevier
external identifiers
  • wos:000288469000022
  • scopus:79651475389
ISSN
1532-2165
DOI
10.1016/j.ejvs.2010.09.012
language
English
LU publication?
yes
id
a2922415-7421-4277-b83a-fae9125432bb (old id 1936224)
date added to LUP
2011-05-02 08:00:55
date last changed
2017-01-01 06:35:52
@article{a2922415-7421-4277-b83a-fae9125432bb,
  abstract     = {Introduction: Messenger RNA (mRNA) changes in the small intestine in response to acute mesenteric ischaemia (AMI) could offer novel diagnostic possibilities, but have not been described. The aim was to characterize the mRNA response to experimental AMI. Materials and methods: Twelve pigs underwent catheterisation of the superior mesenteric artery with injection of polivinylalcohol embolisation particles or sodium chloride. Laparotomy and intestinal tissue sampling were performed. Microarray analysis was performed using the GeneChip (R) whole porcine genome array. Results: Seven down-regulated cellular pathways were associated with protein, lipid and carbohydrate metabolism. Seventeen up-regulated pathways were associated with inflammatory and immunological activity, regulation of extracellular matrix and decreased cellular proliferation. Thrombospondin (THS), monocyte chemoattractant protein 1(MCP-1) and gap junction alpha 1(GJA-1) were consistently up-regulated in all embolised pigs. Genes encoding earlier proposed biomarkers for AMI were up-regulated, such as lactate dehydrogenase and creatine kinase, or down-regulated, such as intestinal fatty acid binding protein and glutathione S-transferase. Conclusion: This study describes the intestinal tissue response on a gene expression level to AMI. THS, MCP-1 and GJA-1 were consistently up-regulated by ischaemia, whereas earlier proposed biomarkers for AMI were not. Gene expression may not be directly linked to the use of the corresponding proteins as potential clinical biomarkers. (C) 2010 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.},
  author       = {Block, T. and Isaksson, H. S. and Acosta, Stefan and Bjorck, M. and Brodin, D. and Nilsson, T. K.},
  issn         = {1532-2165},
  keyword      = {Superior mesenteric artery,Acute mesenteric ischaemia,Gene expression,analysis,Porcine,Microarray},
  language     = {eng},
  number       = {2},
  pages        = {281--287},
  publisher    = {Elsevier},
  series       = {European Journal of Vascular and Endovascular Surgery},
  title        = {Altered mRNA Expression due to Acute Mesenteric Ischaemia in a Porcine Model},
  url          = {http://dx.doi.org/10.1016/j.ejvs.2010.09.012},
  volume       = {41},
  year         = {2011},
}