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Nanoparticle-based pseudostationary phases in CEC: A breakthrough in protein analysis?

Nilsson, Christian LU ; Birnbaum, Staffan and Nilsson, Staffan LU (2011) In Electrophoresis 32(10). p.1141-1147
Abstract
This review focuses on major trends in nanoparticle-based pseudostationary phase (PSP) CEC since the publication of our previous reviews within nanoparticle-based CEC [Nilsson, C., et al., Electrophoresis 2006, 27, 76-83; Nilsson, C., et al., J. Chromatogr. A 2007, 1168, 212-224.]. Special attention is given to the development toward protein analysis, which is driven by the strong emergence of protein drug development in the pharmaceutical industry. Furthermore, we discuss the development in coupling different detection techniques with nanoparticle-based PSP CEC, which were originally predicted to be particularly cumbersome. However, at present, direct UV, LIF and ESI-MS have been used without any severe complications. Different types of... (More)
This review focuses on major trends in nanoparticle-based pseudostationary phase (PSP) CEC since the publication of our previous reviews within nanoparticle-based CEC [Nilsson, C., et al., Electrophoresis 2006, 27, 76-83; Nilsson, C., et al., J. Chromatogr. A 2007, 1168, 212-224.]. Special attention is given to the development toward protein analysis, which is driven by the strong emergence of protein drug development in the pharmaceutical industry. Furthermore, we discuss the development in coupling different detection techniques with nanoparticle-based PSP CEC, which were originally predicted to be particularly cumbersome. However, at present, direct UV, LIF and ESI-MS have been used without any severe complications. Different types of nanoparticles used as PSP during the period include gold nanoparticles, carbon nanostructures and lipid-based nanoparticles. New materials (for example, different types of carbon nanostructures and self assembled lipid-based nanostructures) are a strong driving force for development in separation science. Finally, future trends in nanoparticles-based CEC are envisioned. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Capillary electrochromatography, CE, Nanoparticles, Proteins, Pseudostationary phase
in
Electrophoresis
volume
32
issue
10
pages
1141 - 1147
publisher
John Wiley & Sons
external identifiers
  • wos:000290586200006
  • pmid:21500215
  • scopus:79955609250
ISSN
0173-0835
DOI
10.1002/elps.201000645
language
English
LU publication?
yes
id
e303076f-9823-4c7c-9926-4d35c361261b (old id 1936976)
date added to LUP
2011-05-24 17:26:38
date last changed
2017-10-22 03:26:23
@article{e303076f-9823-4c7c-9926-4d35c361261b,
  abstract     = {This review focuses on major trends in nanoparticle-based pseudostationary phase (PSP) CEC since the publication of our previous reviews within nanoparticle-based CEC [Nilsson, C., et al., Electrophoresis 2006, 27, 76-83; Nilsson, C., et al., J. Chromatogr. A 2007, 1168, 212-224.]. Special attention is given to the development toward protein analysis, which is driven by the strong emergence of protein drug development in the pharmaceutical industry. Furthermore, we discuss the development in coupling different detection techniques with nanoparticle-based PSP CEC, which were originally predicted to be particularly cumbersome. However, at present, direct UV, LIF and ESI-MS have been used without any severe complications. Different types of nanoparticles used as PSP during the period include gold nanoparticles, carbon nanostructures and lipid-based nanoparticles. New materials (for example, different types of carbon nanostructures and self assembled lipid-based nanostructures) are a strong driving force for development in separation science. Finally, future trends in nanoparticles-based CEC are envisioned.},
  author       = {Nilsson, Christian and Birnbaum, Staffan and Nilsson, Staffan},
  issn         = {0173-0835},
  keyword      = {Capillary electrochromatography,CE,Nanoparticles,Proteins,Pseudostationary phase},
  language     = {eng},
  number       = {10},
  pages        = {1141--1147},
  publisher    = {John Wiley & Sons},
  series       = {Electrophoresis},
  title        = {Nanoparticle-based pseudostationary phases in CEC: A breakthrough in protein analysis?},
  url          = {http://dx.doi.org/10.1002/elps.201000645},
  volume       = {32},
  year         = {2011},
}