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Excitation-Emission Polarization Spectroscopy of Single Light Harvesting Complexes.

Tubasum, Sumera LU ; Cogdell, Richard J; Scheblykin, Ivan LU and Pullerits, Tönu LU (2011) In The Journal of Physical Chemistry Part B 115(17). p.4963-4970
Abstract
Excitation and emission polarization dependence of fluorescence intensity of single LH2 complexes from Rhodopseudomonas acidophila 10050 and Rhodobacter sphaeroides is reported. The results are presented as two-dimensional polarization plots and interpreted in terms of tilted light harvesting complexes indicating that sample preparation leads to partially oriented LH2 cylinders. An alternative explanation of the observation can be structural deformation. Fluorescence intensity of the complexes has four qualitatively distinct excitation-emission polarization dependencies. The differences in excitation polarization dependence are interpreted as due to the tilt of the complexes, whereas the emission polarization behavior is mainly determined... (More)
Excitation and emission polarization dependence of fluorescence intensity of single LH2 complexes from Rhodopseudomonas acidophila 10050 and Rhodobacter sphaeroides is reported. The results are presented as two-dimensional polarization plots and interpreted in terms of tilted light harvesting complexes indicating that sample preparation leads to partially oriented LH2 cylinders. An alternative explanation of the observation can be structural deformation. Fluorescence intensity of the complexes has four qualitatively distinct excitation-emission polarization dependencies. The differences in excitation polarization dependence are interpreted as due to the tilt of the complexes, whereas the emission polarization behavior is mainly determined by spectral inhomogeneity of the emitting B850 ring. Some complexes show abrupt reversible variations of the total emission intensity together with changes of the polarization properties which cannot be described by the simplest model of tilted LH2s with spectral disorder. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The Journal of Physical Chemistry Part B
volume
115
issue
17
pages
4963 - 4970
publisher
The American Chemical Society
external identifiers
  • wos:000289824300008
  • pmid:21486039
  • scopus:79955488490
ISSN
1520-5207
DOI
10.1021/jp107480x
language
English
LU publication?
yes
id
716aae84-1320-4417-b1fe-2436cf1c9069 (old id 1937230)
date added to LUP
2011-05-24 17:47:30
date last changed
2017-01-15 03:52:43
@article{716aae84-1320-4417-b1fe-2436cf1c9069,
  abstract     = {Excitation and emission polarization dependence of fluorescence intensity of single LH2 complexes from Rhodopseudomonas acidophila 10050 and Rhodobacter sphaeroides is reported. The results are presented as two-dimensional polarization plots and interpreted in terms of tilted light harvesting complexes indicating that sample preparation leads to partially oriented LH2 cylinders. An alternative explanation of the observation can be structural deformation. Fluorescence intensity of the complexes has four qualitatively distinct excitation-emission polarization dependencies. The differences in excitation polarization dependence are interpreted as due to the tilt of the complexes, whereas the emission polarization behavior is mainly determined by spectral inhomogeneity of the emitting B850 ring. Some complexes show abrupt reversible variations of the total emission intensity together with changes of the polarization properties which cannot be described by the simplest model of tilted LH2s with spectral disorder.},
  author       = {Tubasum, Sumera and Cogdell, Richard J and Scheblykin, Ivan and Pullerits, Tönu},
  issn         = {1520-5207},
  language     = {eng},
  number       = {17},
  pages        = {4963--4970},
  publisher    = {The American Chemical Society},
  series       = {The Journal of Physical Chemistry Part B},
  title        = {Excitation-Emission Polarization Spectroscopy of Single Light Harvesting Complexes.},
  url          = {http://dx.doi.org/10.1021/jp107480x},
  volume       = {115},
  year         = {2011},
}