Advanced

Growth of saprotrophic fungi and bacteria in soil.

Rousk, Johannes LU and Bååth, Erland LU (2011) In FEMS Microbiology Ecology 78. p.17-30
Abstract
Bacterial and fungal growth rate measurements are sensitive variables to detect changes in environmental conditions. However, while methods to assess the species composition and biomass of fungi and bacteria has made much progress, information about growth rates remains surprisingly rudimentary. We review the recent history of approaches to assess bacterial and fungal growth rates, leading up to current methods, especially focusing on leucine/thymidine incorporation to estimate bacterial growth and acetate incorporation into ergosterol to estimate fungal growth. We present underlying assumptions for these methods, compare estimates of turnover times for fungi and bacteria based on them, and discuss issues, including e.g. elusive conversion... (More)
Bacterial and fungal growth rate measurements are sensitive variables to detect changes in environmental conditions. However, while methods to assess the species composition and biomass of fungi and bacteria has made much progress, information about growth rates remains surprisingly rudimentary. We review the recent history of approaches to assess bacterial and fungal growth rates, leading up to current methods, especially focusing on leucine/thymidine incorporation to estimate bacterial growth and acetate incorporation into ergosterol to estimate fungal growth. We present underlying assumptions for these methods, compare estimates of turnover times for fungi and bacteria based on them, and discuss issues, including e.g. elusive conversion factors. We review what the application of fungal and bacterial growth rate methods has revealed regarding the influence of the environmental factors of temperature, moisture (including drying/rewetting), pH, as well as the influence of substrate additions, presence of plants and toxins. We highlight experiments exploring competitive and facilitative interaction between bacteria and fungi enabled using growth rate methods. Finally, we predict that growth methods will be an important complement to molecular approaches to elucidate fungal and bacterial ecology, and we identify methodological concerns and how they should be addressed. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
fungal growth, bacterial growth, leucine/thymidine incorporation, acetate in ergosterol incorporation, turnover time, biomass production.
in
FEMS Microbiology Ecology
volume
78
pages
17 - 30
publisher
Elsevier
external identifiers
  • wos:000294921800003
  • scopus:80052730357
ISSN
1574-6941
DOI
10.1111/j.1574-6941.2011.01106.x
project
Microbial carbon-use efficiency
Effect of environmental factors on fungal and bacterial growth in soil
Interaction between fungi and bacteria in soil
Responses of soil microbes to drought and rewetting
BECC
language
English
LU publication?
yes
id
d21450a6-12c1-4322-934c-44413a5cf562 (old id 1937443)
date added to LUP
2011-05-17 14:53:17
date last changed
2017-10-01 04:02:46
@article{d21450a6-12c1-4322-934c-44413a5cf562,
  abstract     = {Bacterial and fungal growth rate measurements are sensitive variables to detect changes in environmental conditions. However, while methods to assess the species composition and biomass of fungi and bacteria has made much progress, information about growth rates remains surprisingly rudimentary. We review the recent history of approaches to assess bacterial and fungal growth rates, leading up to current methods, especially focusing on leucine/thymidine incorporation to estimate bacterial growth and acetate incorporation into ergosterol to estimate fungal growth. We present underlying assumptions for these methods, compare estimates of turnover times for fungi and bacteria based on them, and discuss issues, including e.g. elusive conversion factors. We review what the application of fungal and bacterial growth rate methods has revealed regarding the influence of the environmental factors of temperature, moisture (including drying/rewetting), pH, as well as the influence of substrate additions, presence of plants and toxins. We highlight experiments exploring competitive and facilitative interaction between bacteria and fungi enabled using growth rate methods. Finally, we predict that growth methods will be an important complement to molecular approaches to elucidate fungal and bacterial ecology, and we identify methodological concerns and how they should be addressed.},
  author       = {Rousk, Johannes and Bååth, Erland},
  issn         = {1574-6941},
  keyword      = {fungal growth,bacterial growth,leucine/thymidine incorporation,acetate in ergosterol incorporation,turnover time,biomass production.},
  language     = {eng},
  pages        = {17--30},
  publisher    = {Elsevier},
  series       = {FEMS Microbiology Ecology},
  title        = {Growth of saprotrophic fungi and bacteria in soil.},
  url          = {http://dx.doi.org/10.1111/j.1574-6941.2011.01106.x},
  volume       = {78},
  year         = {2011},
}