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Bioprospecting Microbial Diversity for Lignin Valorization : Dry and Wet Screening Methods

Gonçalves, Carolyne Caetano ; Bruce, Thiago ; Silva, Caio de Oliveira Gorgulho ; Fillho, Edivaldo Ximenes Ferreira ; Noronha, Eliane Ferreira ; Carlquist, Magnus LU and Parachin, Nádia Skorupa LU (2020) In Frontiers in Microbiology 11.
Abstract

Lignin is an abundant cell wall component, and it has been used mainly for generating steam and electricity. Nevertheless, lignin valorization, i.e. the conversion of lignin into high value-added fuels, chemicals, or materials, is crucial for the full implementation of cost-effective lignocellulosic biorefineries. From this perspective, rapid screening methods are crucial for time- and resource-efficient development of novel microbial strains and enzymes with applications in the lignin biorefinery. The present review gives an overview of recent developments and applications of a vast arsenal of activity and sequence-based methodologies for uncovering novel microbial strains with ligninolytic potential, novel enzymes for lignin... (More)

Lignin is an abundant cell wall component, and it has been used mainly for generating steam and electricity. Nevertheless, lignin valorization, i.e. the conversion of lignin into high value-added fuels, chemicals, or materials, is crucial for the full implementation of cost-effective lignocellulosic biorefineries. From this perspective, rapid screening methods are crucial for time- and resource-efficient development of novel microbial strains and enzymes with applications in the lignin biorefinery. The present review gives an overview of recent developments and applications of a vast arsenal of activity and sequence-based methodologies for uncovering novel microbial strains with ligninolytic potential, novel enzymes for lignin depolymerization and for unraveling the main metabolic routes during growth on lignin. Finally, perspectives on the use of each of the presented methods and their respective advantages and disadvantages are discussed.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
activity-based screening, bioprocess, biosensors, high-throughput screening, lignin-degrading enzymes
in
Frontiers in Microbiology
volume
11
article number
1081
publisher
Frontiers Media S. A.
external identifiers
  • pmid:32582068
  • scopus:85087041763
ISSN
1664-302X
DOI
10.3389/fmicb.2020.01081
language
English
LU publication?
yes
id
19395441-59f5-464e-8311-d9265cfb9093
date added to LUP
2020-07-08 10:26:59
date last changed
2024-06-12 17:04:04
@article{19395441-59f5-464e-8311-d9265cfb9093,
  abstract     = {{<p>Lignin is an abundant cell wall component, and it has been used mainly for generating steam and electricity. Nevertheless, lignin valorization, i.e. the conversion of lignin into high value-added fuels, chemicals, or materials, is crucial for the full implementation of cost-effective lignocellulosic biorefineries. From this perspective, rapid screening methods are crucial for time- and resource-efficient development of novel microbial strains and enzymes with applications in the lignin biorefinery. The present review gives an overview of recent developments and applications of a vast arsenal of activity and sequence-based methodologies for uncovering novel microbial strains with ligninolytic potential, novel enzymes for lignin depolymerization and for unraveling the main metabolic routes during growth on lignin. Finally, perspectives on the use of each of the presented methods and their respective advantages and disadvantages are discussed.</p>}},
  author       = {{Gonçalves, Carolyne Caetano and Bruce, Thiago and Silva, Caio de Oliveira Gorgulho and Fillho, Edivaldo Ximenes Ferreira and Noronha, Eliane Ferreira and Carlquist, Magnus and Parachin, Nádia Skorupa}},
  issn         = {{1664-302X}},
  keywords     = {{activity-based screening; bioprocess; biosensors; high-throughput screening; lignin-degrading enzymes}},
  language     = {{eng}},
  month        = {{06}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Microbiology}},
  title        = {{Bioprospecting Microbial Diversity for Lignin Valorization : Dry and Wet Screening Methods}},
  url          = {{http://dx.doi.org/10.3389/fmicb.2020.01081}},
  doi          = {{10.3389/fmicb.2020.01081}},
  volume       = {{11}},
  year         = {{2020}},
}