Bioprospecting Microbial Diversity for Lignin Valorization : Dry and Wet Screening Methods
(2020) In Frontiers in Microbiology 11.- Abstract
Lignin is an abundant cell wall component, and it has been used mainly for generating steam and electricity. Nevertheless, lignin valorization, i.e. the conversion of lignin into high value-added fuels, chemicals, or materials, is crucial for the full implementation of cost-effective lignocellulosic biorefineries. From this perspective, rapid screening methods are crucial for time- and resource-efficient development of novel microbial strains and enzymes with applications in the lignin biorefinery. The present review gives an overview of recent developments and applications of a vast arsenal of activity and sequence-based methodologies for uncovering novel microbial strains with ligninolytic potential, novel enzymes for lignin... (More)
Lignin is an abundant cell wall component, and it has been used mainly for generating steam and electricity. Nevertheless, lignin valorization, i.e. the conversion of lignin into high value-added fuels, chemicals, or materials, is crucial for the full implementation of cost-effective lignocellulosic biorefineries. From this perspective, rapid screening methods are crucial for time- and resource-efficient development of novel microbial strains and enzymes with applications in the lignin biorefinery. The present review gives an overview of recent developments and applications of a vast arsenal of activity and sequence-based methodologies for uncovering novel microbial strains with ligninolytic potential, novel enzymes for lignin depolymerization and for unraveling the main metabolic routes during growth on lignin. Finally, perspectives on the use of each of the presented methods and their respective advantages and disadvantages are discussed.
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- author
- Gonçalves, Carolyne Caetano ; Bruce, Thiago ; Silva, Caio de Oliveira Gorgulho ; Fillho, Edivaldo Ximenes Ferreira ; Noronha, Eliane Ferreira ; Carlquist, Magnus LU and Parachin, Nádia Skorupa LU
- organization
- publishing date
- 2020-06-09
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- activity-based screening, bioprocess, biosensors, high-throughput screening, lignin-degrading enzymes
- in
- Frontiers in Microbiology
- volume
- 11
- article number
- 1081
- publisher
- Frontiers Media S. A.
- external identifiers
-
- pmid:32582068
- scopus:85087041763
- ISSN
- 1664-302X
- DOI
- 10.3389/fmicb.2020.01081
- language
- English
- LU publication?
- yes
- id
- 19395441-59f5-464e-8311-d9265cfb9093
- date added to LUP
- 2020-07-08 10:26:59
- date last changed
- 2024-06-12 17:04:04
@article{19395441-59f5-464e-8311-d9265cfb9093, abstract = {{<p>Lignin is an abundant cell wall component, and it has been used mainly for generating steam and electricity. Nevertheless, lignin valorization, i.e. the conversion of lignin into high value-added fuels, chemicals, or materials, is crucial for the full implementation of cost-effective lignocellulosic biorefineries. From this perspective, rapid screening methods are crucial for time- and resource-efficient development of novel microbial strains and enzymes with applications in the lignin biorefinery. The present review gives an overview of recent developments and applications of a vast arsenal of activity and sequence-based methodologies for uncovering novel microbial strains with ligninolytic potential, novel enzymes for lignin depolymerization and for unraveling the main metabolic routes during growth on lignin. Finally, perspectives on the use of each of the presented methods and their respective advantages and disadvantages are discussed.</p>}}, author = {{Gonçalves, Carolyne Caetano and Bruce, Thiago and Silva, Caio de Oliveira Gorgulho and Fillho, Edivaldo Ximenes Ferreira and Noronha, Eliane Ferreira and Carlquist, Magnus and Parachin, Nádia Skorupa}}, issn = {{1664-302X}}, keywords = {{activity-based screening; bioprocess; biosensors; high-throughput screening; lignin-degrading enzymes}}, language = {{eng}}, month = {{06}}, publisher = {{Frontiers Media S. A.}}, series = {{Frontiers in Microbiology}}, title = {{Bioprospecting Microbial Diversity for Lignin Valorization : Dry and Wet Screening Methods}}, url = {{http://dx.doi.org/10.3389/fmicb.2020.01081}}, doi = {{10.3389/fmicb.2020.01081}}, volume = {{11}}, year = {{2020}}, }