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Material characterization and in vivo behavior of silicon substituted alpha-tricalcium phosphate cement.

Camire, Christopher LU ; Jegou Saint-Jean, Simon LU ; Mochales, Carolina; Nevsten, Pernilla LU ; Wang, Jian-Sheng LU ; Lidgren, Lars LU ; McCarthy, Ian LU and Ginebra, Maria-Pau (2006) In Journal of Biomedical Materials Research. Part B - Applied Biomaterials 76B(2). p.424-431
Abstract
The possibility and biological effects of substituting silicon in a-tricalcium phosphate (alpha-TCP) by way of solid-state reaction have been evaluated. a-TCP powders with varying substitution amounts (1 and 5 mol % Ca2SiO4) were synthesized by reacting mixtures of CaCO3, Ca(2)P(2)O7, and SiO2, at a rate of 4 degrees C(min)(-1) to 1100 degrees C, left to dwell for 2 h and then heated to 1325 degrees C at 4 degrees C(min)(-1) and left to dwell for a period of 4 h. The powders were then rapidly quenched in air. Si incorporation could be verified by X-ray diffraction analysis, indicating an increase of the lattice volume with increasing Si content from 4284.1(8) to 4334(1) angstrom(3) for pure alpha-TCP and alpha-Si5%TCP, respectively. The... (More)
The possibility and biological effects of substituting silicon in a-tricalcium phosphate (alpha-TCP) by way of solid-state reaction have been evaluated. a-TCP powders with varying substitution amounts (1 and 5 mol % Ca2SiO4) were synthesized by reacting mixtures of CaCO3, Ca(2)P(2)O7, and SiO2, at a rate of 4 degrees C(min)(-1) to 1100 degrees C, left to dwell for 2 h and then heated to 1325 degrees C at 4 degrees C(min)(-1) and left to dwell for a period of 4 h. The powders were then rapidly quenched in air. Si incorporation could be verified by X-ray diffraction analysis, indicating an increase of the lattice volume with increasing Si content from 4284.1(8) to 4334(1) angstrom(3) for pure alpha-TCP and alpha-Si5%TCP, respectively. The hydrolysis of milled alpha-SiTCP powders was monitored by isothermal calorimetry, and the compressive strength of set cements was tested. The results showed changes in speed and amount of heat released during reactivity tests and a decrease in mechanical strength (60, 50, and 5 MPa) with increasing Si content. In vitro bioactivity of the set cements after soaking in simulated body fluid for 4 weeks was also tested. The formation of a bonelike apatite layer on the surface of the set cements could be observed and was thickest for 1% Si (20 mu m). These results were in good agreement with the in vivo studies performed, which showed strong evidence that the cement containing 1% silicon doped alpha-TCP enhanced mesenchymal cell differentiation and increased osteoblast activity compared with alpha-TCP. (c) 2005 Wiley Periodicals, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bioactivity, bone formation, deficient hydroxyapatite (CDHA), silicon-substituted alpha-tricalcium phosphate (alpha-TCP), calcium, reactivity
in
Journal of Biomedical Materials Research. Part B - Applied Biomaterials
volume
76B
issue
2
pages
424 - 431
publisher
John Wiley & Sons
external identifiers
  • pmid:16184531
  • wos:000234834700024
  • scopus:31644450909
ISSN
1552-4981
DOI
10.1002/jbm.b.30385
language
English
LU publication?
yes
id
195c245f-acba-432a-bd22-8167900b0962 (old id 143427)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16184531&dopt=Abstract
date added to LUP
2007-07-12 09:33:13
date last changed
2019-03-19 01:46:36
@article{195c245f-acba-432a-bd22-8167900b0962,
  abstract     = {The possibility and biological effects of substituting silicon in a-tricalcium phosphate (alpha-TCP) by way of solid-state reaction have been evaluated. a-TCP powders with varying substitution amounts (1 and 5 mol % Ca2SiO4) were synthesized by reacting mixtures of CaCO3, Ca(2)P(2)O7, and SiO2, at a rate of 4 degrees C(min)(-1) to 1100 degrees C, left to dwell for 2 h and then heated to 1325 degrees C at 4 degrees C(min)(-1) and left to dwell for a period of 4 h. The powders were then rapidly quenched in air. Si incorporation could be verified by X-ray diffraction analysis, indicating an increase of the lattice volume with increasing Si content from 4284.1(8) to 4334(1) angstrom(3) for pure alpha-TCP and alpha-Si5%TCP, respectively. The hydrolysis of milled alpha-SiTCP powders was monitored by isothermal calorimetry, and the compressive strength of set cements was tested. The results showed changes in speed and amount of heat released during reactivity tests and a decrease in mechanical strength (60, 50, and 5 MPa) with increasing Si content. In vitro bioactivity of the set cements after soaking in simulated body fluid for 4 weeks was also tested. The formation of a bonelike apatite layer on the surface of the set cements could be observed and was thickest for 1% Si (20 mu m). These results were in good agreement with the in vivo studies performed, which showed strong evidence that the cement containing 1% silicon doped alpha-TCP enhanced mesenchymal cell differentiation and increased osteoblast activity compared with alpha-TCP. (c) 2005 Wiley Periodicals, Inc.},
  author       = {Camire, Christopher and Jegou Saint-Jean, Simon and Mochales, Carolina and Nevsten, Pernilla and Wang, Jian-Sheng and Lidgren, Lars and McCarthy, Ian and Ginebra, Maria-Pau},
  issn         = {1552-4981},
  keyword      = {bioactivity,bone formation,deficient hydroxyapatite (CDHA),silicon-substituted alpha-tricalcium phosphate (alpha-TCP),calcium,reactivity},
  language     = {eng},
  number       = {2},
  pages        = {424--431},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Biomedical Materials Research. Part B - Applied Biomaterials},
  title        = {Material characterization and in vivo behavior of silicon substituted alpha-tricalcium phosphate cement.},
  url          = {http://dx.doi.org/10.1002/jbm.b.30385},
  volume       = {76B},
  year         = {2006},
}