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Inhibition of splicing by serine-arginine rich protein 55 (SRp55) causes the appearance of partially spliced HIV-1 mRNAs in the cytoplasm

Tranell, Anna; Tingsborg, Susanne; Fenyö, Eva Maria LU and Schwartz, Stefan LU (2011) In Virus Research 157(1). p.82-91
Abstract
We have previously shown that SRp55 inhibits splicing from HIV-1 exon 3, thereby generating partially spliced mRNAs encoding HIV-1 vpr. Here we show that SRp55 also inhibits splicing from HIV-1 exon 5 to generate HIV-1 vpu/env mRNA, albeit with lower efficiency. We also show that inhibition of HIV-1 splicing by SRp55 causes the appearance of partially spliced vpu, env and vpr mRNAs in the cytoplasm. SRp55 could also induce production of extracellular p24gag from a rev-defective HIV-1 provirus. These results indicate that SRp55 aids in the generation of partially spliced and unspliced HIV-1 mRNAs. (C) 2011 Elsevier B.V. All rights reserved.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
HIV-1, vpr, SRp55, Splicing
in
Virus Research
volume
157
issue
1
pages
82 - 91
publisher
Elsevier
external identifiers
  • wos:000289864600011
  • scopus:79953024872
ISSN
1872-7492
DOI
10.1016/j.virusres.2011.02.010
language
English
LU publication?
yes
id
8655d89e-8339-4122-8bae-c2fe770ee404 (old id 1962857)
date added to LUP
2011-05-16 15:40:39
date last changed
2017-04-09 03:04:45
@article{8655d89e-8339-4122-8bae-c2fe770ee404,
  abstract     = {We have previously shown that SRp55 inhibits splicing from HIV-1 exon 3, thereby generating partially spliced mRNAs encoding HIV-1 vpr. Here we show that SRp55 also inhibits splicing from HIV-1 exon 5 to generate HIV-1 vpu/env mRNA, albeit with lower efficiency. We also show that inhibition of HIV-1 splicing by SRp55 causes the appearance of partially spliced vpu, env and vpr mRNAs in the cytoplasm. SRp55 could also induce production of extracellular p24gag from a rev-defective HIV-1 provirus. These results indicate that SRp55 aids in the generation of partially spliced and unspliced HIV-1 mRNAs. (C) 2011 Elsevier B.V. All rights reserved.},
  author       = {Tranell, Anna and Tingsborg, Susanne and Fenyö, Eva Maria and Schwartz, Stefan},
  issn         = {1872-7492},
  keyword      = {HIV-1,vpr,SRp55,Splicing},
  language     = {eng},
  number       = {1},
  pages        = {82--91},
  publisher    = {Elsevier},
  series       = {Virus Research},
  title        = {Inhibition of splicing by serine-arginine rich protein 55 (SRp55) causes the appearance of partially spliced HIV-1 mRNAs in the cytoplasm},
  url          = {http://dx.doi.org/10.1016/j.virusres.2011.02.010},
  volume       = {157},
  year         = {2011},
}