Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Binding studies on substrate- and enantio-selective molecularly imprinted polymers

Kempe, Maria LU and Mosbach, Klaus LU (1991) In Analytical Letters 24(7). p.1137-1145
Abstract
A molecularly imprinted polymer was prepared using tert-butyloxycarbonyl-L-phenylalanine as the print molecule and methacrylic acid as the functional monomer. The bulk polymer obtained was ground, sieved, packed into a column and investigated in the HPLC-mode by frontal chromatography to determine the number of binding sites and dissociation constants for the enantiomers interacting with the polymer. The dissociation constant for the L-enantiomer of the print molecule was lower than for the D-enantiomer (6.3 mM and 8.1 mM, respectively). This means that the affinity for the L-enantiomer was higher than for the D-enantiomer. The number of binding sites in the polymer giving rise to these dissociation constants were determined to be 28 μmol... (More)
A molecularly imprinted polymer was prepared using tert-butyloxycarbonyl-L-phenylalanine as the print molecule and methacrylic acid as the functional monomer. The bulk polymer obtained was ground, sieved, packed into a column and investigated in the HPLC-mode by frontal chromatography to determine the number of binding sites and dissociation constants for the enantiomers interacting with the polymer. The dissociation constant for the L-enantiomer of the print molecule was lower than for the D-enantiomer (6.3 mM and 8.1 mM, respectively). This means that the affinity for the L-enantiomer was higher than for the D-enantiomer. The number of binding sites in the polymer giving rise to these dissociation constants were determined to be 28 μmol per g dry polymer. (Less)
Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Letters
volume
24
issue
7
pages
1137 - 1145
publisher
Taylor & Francis
external identifiers
  • scopus:0000665502
ISSN
0003-2719
DOI
10.1080/00032719108052959
language
English
LU publication?
yes
id
197029d0-7438-4e4f-a4a3-1f197ff20c31 (old id 1516052)
date added to LUP
2016-04-01 15:53:30
date last changed
2021-08-29 05:18:41
@article{197029d0-7438-4e4f-a4a3-1f197ff20c31,
  abstract     = {{A molecularly imprinted polymer was prepared using tert-butyloxycarbonyl-L-phenylalanine as the print molecule and methacrylic acid as the functional monomer. The bulk polymer obtained was ground, sieved, packed into a column and investigated in the HPLC-mode by frontal chromatography to determine the number of binding sites and dissociation constants for the enantiomers interacting with the polymer. The dissociation constant for the L-enantiomer of the print molecule was lower than for the D-enantiomer (6.3 mM and 8.1 mM, respectively). This means that the affinity for the L-enantiomer was higher than for the D-enantiomer. The number of binding sites in the polymer giving rise to these dissociation constants were determined to be 28 μmol per g dry polymer.}},
  author       = {{Kempe, Maria and Mosbach, Klaus}},
  issn         = {{0003-2719}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{1137--1145}},
  publisher    = {{Taylor & Francis}},
  series       = {{Analytical Letters}},
  title        = {{Binding studies on substrate- and enantio-selective molecularly imprinted polymers}},
  url          = {{http://dx.doi.org/10.1080/00032719108052959}},
  doi          = {{10.1080/00032719108052959}},
  volume       = {{24}},
  year         = {{1991}},
}