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Termitomyces sp. associated with the termite Macrotermes natalensis has a heterothallic mating system and multinucleate cells

de Fine Licht, Henrik Hjarvard LU ; Andersen, A. and Aanen, D.K. (2005) In Mycological Research 109(3). p.314-318
Abstract
Fungi of the genus Termitomyces live in an obligate symbiosis with termites of the subfamily Macrotermitinae. Many species of Termitomyces frequently form fruit bodies, which develop from the fungus comb within the nest. In this study,

we determined the mating system of a species of Termitomyces associated with the South African termite Macrotermes natalensis. Termite nests were excavated and a Termitomyces sp. was isolated into pure culture from the asexual fruit

bodies (nodules) growing in the fungus gardens. For one strain, single basidiospore cultures were obtained from basidiomes growing from the fungus comb after incubation without termites. Using nuclear staining, we show that both

comb cultures and single... (More)
Fungi of the genus Termitomyces live in an obligate symbiosis with termites of the subfamily Macrotermitinae. Many species of Termitomyces frequently form fruit bodies, which develop from the fungus comb within the nest. In this study,

we determined the mating system of a species of Termitomyces associated with the South African termite Macrotermes natalensis. Termite nests were excavated and a Termitomyces sp. was isolated into pure culture from the asexual fruit

bodies (nodules) growing in the fungus gardens. For one strain, single basidiospore cultures were obtained from basidiomes growing from the fungus comb after incubation without termites. Using nuclear staining, we show that both

comb cultures and single spore cultures have multinucleate cells and that the majority of spores has a single nucleus.

However, DNA sequencing of the ITS region in the nuclear RNA gene revealed that the comb mycelium had two different ITS types that segregated in the single spore cultures, which consequently had only a single ITS type. These results unambiguously prove that the strain of Termitomyces studied here has a heterothallic mating system, with the fungus garden of the termite mound being in the heterokaryotic phase. This is the first time the mating system of a

Termitomyces species has been studied. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Mycological Research
volume
109
issue
3
pages
314 - 318
publisher
Cambridge University Press
external identifiers
  • scopus:18244384090
ISSN
1469-8102
DOI
10.1017/S0953756204001844
language
English
LU publication?
no
id
3606e667-57ef-432a-ad7b-603173f39f19 (old id 1970601)
date added to LUP
2011-06-14 15:47:55
date last changed
2017-10-01 03:41:45
@article{3606e667-57ef-432a-ad7b-603173f39f19,
  abstract     = {Fungi of the genus Termitomyces live in an obligate symbiosis with termites of the subfamily Macrotermitinae. Many species of Termitomyces frequently form fruit bodies, which develop from the fungus comb within the nest. In this study,<br/><br>
we determined the mating system of a species of Termitomyces associated with the South African termite Macrotermes natalensis. Termite nests were excavated and a Termitomyces sp. was isolated into pure culture from the asexual fruit<br/><br>
bodies (nodules) growing in the fungus gardens. For one strain, single basidiospore cultures were obtained from basidiomes growing from the fungus comb after incubation without termites. Using nuclear staining, we show that both<br/><br>
comb cultures and single spore cultures have multinucleate cells and that the majority of spores has a single nucleus.<br/><br>
However, DNA sequencing of the ITS region in the nuclear RNA gene revealed that the comb mycelium had two different ITS types that segregated in the single spore cultures, which consequently had only a single ITS type. These results unambiguously prove that the strain of Termitomyces studied here has a heterothallic mating system, with the fungus garden of the termite mound being in the heterokaryotic phase. This is the first time the mating system of a<br/><br>
Termitomyces species has been studied.},
  author       = {de Fine Licht, Henrik Hjarvard and Andersen, A. and Aanen, D.K.},
  issn         = {1469-8102},
  language     = {eng},
  number       = {3},
  pages        = {314--318},
  publisher    = {Cambridge University Press},
  series       = {Mycological Research},
  title        = {Termitomyces sp. associated with the termite Macrotermes natalensis has a heterothallic mating system and multinucleate cells},
  url          = {http://dx.doi.org/10.1017/S0953756204001844},
  volume       = {109},
  year         = {2005},
}