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Patterns of interaction specificity of fungus-growing termites and Termitomyces symbionts in South Africa

Aanen, D. K.; Ros, V. I. D.; de Fine Licht, Henrik Hjarvard LU ; Mitchell, J.; de Beer, Z. W.; Slippers, B.; Rouland-LeFevre, C. and Boomsma, J. J. (2007) In BMC Evolutionary Biology 7(115). p.1-11
Abstract
Background: Termites of the subfamily Macrotermitinae live in a mutualistic symbiosis with basidiomycete fungi of the genus Termitomyces. Here, we explored interaction specificity in fungus-growing termites using samples from 101 colonies in South-Africa and Senegal, belonging to eight species divided over three genera. Knowledge of interaction specificity is important to test the hypothesis that inhabitants (symbionts) are taxonomically less diverse than 'exhabitants' ( hosts) and to test the hypothesis that transmission mode is an important determinant for interaction specificity. Results: Analysis of Molecular Variance among symbiont ITS sequences across termite hosts at three hierarchical levels showed that 47% of the variation... (More)
Background: Termites of the subfamily Macrotermitinae live in a mutualistic symbiosis with basidiomycete fungi of the genus Termitomyces. Here, we explored interaction specificity in fungus-growing termites using samples from 101 colonies in South-Africa and Senegal, belonging to eight species divided over three genera. Knowledge of interaction specificity is important to test the hypothesis that inhabitants (symbionts) are taxonomically less diverse than 'exhabitants' ( hosts) and to test the hypothesis that transmission mode is an important determinant for interaction specificity. Results: Analysis of Molecular Variance among symbiont ITS sequences across termite hosts at three hierarchical levels showed that 47% of the variation occurred between genera, 18% between species, and the remaining 35% between colonies within species. Different patterns of specificity were evident. High mutual specificity was found for the single Macrotermes species studied, as M. natalensis was associated with a single unique fungal haplotype. The three species of the genus Odontotermes showed low symbiont specificity: they were all associated with a genetically diverse set of fungal symbionts, but their fungal symbionts showed some host specificity, as none of the fungal haplotypes were shared between the studied Odontotermes species. Finally, bilaterally low specificity was found for the four tentatively recognized species of the genus Microtermes, which shared and apparently freely exchanged a common pool of divergent fungal symbionts. Conclusion: Interaction specificity was high at the genus level and generally much lower at the species level. A comparison of the observed diversity among fungal symbionts with the diversity among termite hosts, indicated that the fungal symbiont does not follow the general pattern of an endosymbiont, as we found either similar diversity at both sides or higher diversity in the symbiont. Our results further challenge the hypothesis that transmission-mode is a general key-determinant of interaction specificity in fungus-growing termites. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
multiple sequence alignment, phylogenetic-relationships, evolutionary history, ants, macrotermitinae, isoptera, colonies, models, trees, comb
in
BMC Evolutionary Biology
volume
7
issue
115
pages
1 - 11
publisher
BioMed Central
external identifiers
  • scopus:34548387616
ISSN
1471-2148
DOI
10.1186/1471-2148-7-115
language
English
LU publication?
no
id
e81c8108-05b8-4a61-95a6-0e3755f54d5a (old id 1970645)
date added to LUP
2011-06-14 13:49:00
date last changed
2017-08-20 04:25:59
@article{e81c8108-05b8-4a61-95a6-0e3755f54d5a,
  abstract     = {Background: Termites of the subfamily Macrotermitinae live in a mutualistic symbiosis with basidiomycete fungi of the genus Termitomyces. Here, we explored interaction specificity in fungus-growing termites using samples from 101 colonies in South-Africa and Senegal, belonging to eight species divided over three genera. Knowledge of interaction specificity is important to test the hypothesis that inhabitants (symbionts) are taxonomically less diverse than 'exhabitants' ( hosts) and to test the hypothesis that transmission mode is an important determinant for interaction specificity. Results: Analysis of Molecular Variance among symbiont ITS sequences across termite hosts at three hierarchical levels showed that 47% of the variation occurred between genera, 18% between species, and the remaining 35% between colonies within species. Different patterns of specificity were evident. High mutual specificity was found for the single Macrotermes species studied, as M. natalensis was associated with a single unique fungal haplotype. The three species of the genus Odontotermes showed low symbiont specificity: they were all associated with a genetically diverse set of fungal symbionts, but their fungal symbionts showed some host specificity, as none of the fungal haplotypes were shared between the studied Odontotermes species. Finally, bilaterally low specificity was found for the four tentatively recognized species of the genus Microtermes, which shared and apparently freely exchanged a common pool of divergent fungal symbionts. Conclusion: Interaction specificity was high at the genus level and generally much lower at the species level. A comparison of the observed diversity among fungal symbionts with the diversity among termite hosts, indicated that the fungal symbiont does not follow the general pattern of an endosymbiont, as we found either similar diversity at both sides or higher diversity in the symbiont. Our results further challenge the hypothesis that transmission-mode is a general key-determinant of interaction specificity in fungus-growing termites.},
  author       = {Aanen, D. K. and Ros, V. I. D. and de Fine Licht, Henrik Hjarvard and Mitchell, J. and de Beer, Z. W. and Slippers, B. and Rouland-LeFevre, C. and Boomsma, J. J.},
  issn         = {1471-2148},
  keyword      = {multiple sequence alignment,phylogenetic-relationships,evolutionary history,ants,macrotermitinae,isoptera,colonies,models,trees,comb},
  language     = {eng},
  number       = {115},
  pages        = {1--11},
  publisher    = {BioMed Central},
  series       = {BMC Evolutionary Biology},
  title        = {Patterns of interaction specificity of fungus-growing termites and Termitomyces symbionts in South Africa},
  url          = {http://dx.doi.org/10.1186/1471-2148-7-115},
  volume       = {7},
  year         = {2007},
}