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Lectin typing of Campylobacter jejuni using a novel quartz crystal microbalance technique.

Yakovleva, Maria; Moran, Anthony P; Safina, Gulnara; Wadström, Torkel LU and Danielsson, Bengt (2011) In Analytica Chimica Acta 694(1-2). p.1-5
Abstract
Seven Campylobacter jejuni strains were characterised by a lectin typing assay. The typing system was based on a quartz crystal microbalance technique (QCM) with four commercially available lectins (wheat germ agglutinin, Maackia amurensis lectin, Lens culinaris agglutinin, and Concanavalin A), which were chosen for their differing carbohydrate specificities. Initially, the gold surfaces of the quartz crystals were modified with 11-mercaptoundecanoic acid followed by lectin immobilisation using a conventional amine-coupling technique. Bacterial cells were applied for lectin typing without preliminary treatment, and resonant frequency and dissipation responses were recorded. The adhesion of microorganisms on lectin surfaces was confirmed by... (More)
Seven Campylobacter jejuni strains were characterised by a lectin typing assay. The typing system was based on a quartz crystal microbalance technique (QCM) with four commercially available lectins (wheat germ agglutinin, Maackia amurensis lectin, Lens culinaris agglutinin, and Concanavalin A), which were chosen for their differing carbohydrate specificities. Initially, the gold surfaces of the quartz crystals were modified with 11-mercaptoundecanoic acid followed by lectin immobilisation using a conventional amine-coupling technique. Bacterial cells were applied for lectin typing without preliminary treatment, and resonant frequency and dissipation responses were recorded. The adhesion of microorganisms on lectin surfaces was confirmed by atomic force microscopy. Scanning was performed in the tapping mode and the presence of bacteria on lectin-coated surfaces was successfully demonstrated. A significant difference in the dissipation response was observed for different C. jejuni strains which made it possible to use this parameter for discriminating between bacterial strains. In summary, the QCM technique proved a powerful tool for the recognition and discrimination of C. jejuni strains. The approach may also prove applicable to strain discrimination of other bacterial species, particularly pathogens. (Less)
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Contribution to journal
publication status
published
subject
in
Analytica Chimica Acta
volume
694
issue
1-2
pages
1 - 5
publisher
Elsevier
external identifiers
  • wos:000291239300001
  • pmid:21565296
  • scopus:79955706900
ISSN
1873-4324
DOI
10.1016/j.aca.2011.03.014
language
English
LU publication?
yes
id
2897520b-c00c-4a12-bf43-8b164c411a7f (old id 1972695)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21565296?dopt=Abstract
date added to LUP
2011-06-07 19:01:07
date last changed
2017-03-19 04:22:38
@article{2897520b-c00c-4a12-bf43-8b164c411a7f,
  abstract     = {Seven Campylobacter jejuni strains were characterised by a lectin typing assay. The typing system was based on a quartz crystal microbalance technique (QCM) with four commercially available lectins (wheat germ agglutinin, Maackia amurensis lectin, Lens culinaris agglutinin, and Concanavalin A), which were chosen for their differing carbohydrate specificities. Initially, the gold surfaces of the quartz crystals were modified with 11-mercaptoundecanoic acid followed by lectin immobilisation using a conventional amine-coupling technique. Bacterial cells were applied for lectin typing without preliminary treatment, and resonant frequency and dissipation responses were recorded. The adhesion of microorganisms on lectin surfaces was confirmed by atomic force microscopy. Scanning was performed in the tapping mode and the presence of bacteria on lectin-coated surfaces was successfully demonstrated. A significant difference in the dissipation response was observed for different C. jejuni strains which made it possible to use this parameter for discriminating between bacterial strains. In summary, the QCM technique proved a powerful tool for the recognition and discrimination of C. jejuni strains. The approach may also prove applicable to strain discrimination of other bacterial species, particularly pathogens.},
  author       = {Yakovleva, Maria and Moran, Anthony P and Safina, Gulnara and Wadström, Torkel and Danielsson, Bengt},
  issn         = {1873-4324},
  language     = {eng},
  number       = {1-2},
  pages        = {1--5},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {Lectin typing of Campylobacter jejuni using a novel quartz crystal microbalance technique.},
  url          = {http://dx.doi.org/10.1016/j.aca.2011.03.014},
  volume       = {694},
  year         = {2011},
}