PCR-mediated deletion of plasmid DNA
(2008) In Analytical Biochemistry 375(2). p.373-375- Abstract
- The PCR-mediated plasmid DNA deletion method is a simple approach to delete DNA sequences from plasmids using only one round of PCR, with two primers, and without ligation or purification prior to in vivo recombination. By using only PCR, the method is sequence independent and, as shown in this study, is applicable to various sizes of plasmids and deletions using minimal primer design.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/939116
- author
- Hansson, Mattias LU ; Rzeznicka, Kamila LU ; Rosenbäck, Matilda ; Hansson, Mats LU and Sirijovski, Nick LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Biochemistry
- volume
- 375
- issue
- 2
- pages
- 373 - 375
- publisher
- Elsevier
- external identifiers
-
- pmid:18157935
- wos:000254410800029
- scopus:40649102417
- ISSN
- 1096-0309
- DOI
- 10.1016/j.ab.2007.12.005
- language
- English
- LU publication?
- yes
- id
- 19741943-5a8d-4bd5-91b4-94f37a229269 (old id 939116)
- date added to LUP
- 2016-04-01 11:40:35
- date last changed
- 2022-03-28 01:25:59
@article{19741943-5a8d-4bd5-91b4-94f37a229269, abstract = {{The PCR-mediated plasmid DNA deletion method is a simple approach to delete DNA sequences from plasmids using only one round of PCR, with two primers, and without ligation or purification prior to in vivo recombination. By using only PCR, the method is sequence independent and, as shown in this study, is applicable to various sizes of plasmids and deletions using minimal primer design.}}, author = {{Hansson, Mattias and Rzeznicka, Kamila and Rosenbäck, Matilda and Hansson, Mats and Sirijovski, Nick}}, issn = {{1096-0309}}, language = {{eng}}, number = {{2}}, pages = {{373--375}}, publisher = {{Elsevier}}, series = {{Analytical Biochemistry}}, title = {{PCR-mediated deletion of plasmid DNA}}, url = {{http://dx.doi.org/10.1016/j.ab.2007.12.005}}, doi = {{10.1016/j.ab.2007.12.005}}, volume = {{375}}, year = {{2008}}, }